Utilization of microbial cocultures for converting mixed substrates to valuable bioproducts

Construction of Coculture System Containing Escherichia coli with Different Microbial Species for Biochemical Production

“…As described above, the rational modularization and combination of a coculture system containing E. coli with different microbes can complete the complex tasks and improve the chemical production efficiency. However, the nutritional requirements increase the competition relationship between coculture members, resulting in the unstable microbial community composition and even leading to lower production efficiency. , Alternatively, the nutritional divergence within the coculture can completely or partially eliminate the competition, making the coexistence feasible. Generally, glucose and xylose are the major carbohydrates in the lignocellulose.…”

Advances in Polysaccharide Production Based on the Co-Culture of Microbes

“…S. cerevisiae and Scheffersomyces stipitis are useful strains for ethanol production. Here, the former cannot break down xylose, whereas the latter can absorb both sugars and produce bioethanol efficiently in glucose−xylose mixtures [24]. Modularity reduces the metabolic burdens on each component strain, thus contributing to improved overall bioproduction/biotransformation performance [23].…”

Vibrational imaging of metabolites for improved microbial cell strains

“… 14 One of the more potent strategies to induce silenced pathways and enhance the chemodiversity of microorganisms is through microbial co-cultivation, which mimics the competitive environment in unmanaged, natural environments, e.g., the soil rhizosphere and aquatic phycosphere, thus further expanding the variability in possible microbe phenotype. 15 , 16 The severe mismatch between the relative ease of building and cultivating a diverse set of cell variants, versus the tedious process of measuring the metabolite content, as part of the design, build, test, and learn cycle, emphasizes the clear need for improved high-throughput screening technologies. Equally critical is the ability to preserve cell integrity for selective propagation or for the corresponding genetic information and BGCs to be identified through follow-on gene sequencing assays, enabling a more meaningful and comprehensive analysis of the sample.…”