Utilization of fine‐needle aspiration cytology and flow cytometry in the diagnosis and subclassification of primary and recurrent lymphoma

Cytologic findings of marginal zone lymphoma

“…4,6 -9,11,12,15 Large transformed cells also are described frequently, as are the presence of small mature lymphocytes. 6,7,11,12,15 The cytologic characteristics described above are similar to our findings in the specimens from this series and are observed best in the DQ-stained slides, in which subtle differences in cell size and quantity of cytoplasm are enhanced compared with alcohol-fixed H&E or Papstained material.…”

“…[5][6][7][8][9][10][11]15 The consistent contributing factor to an accurate diagnosis of MZL in FNAB reported by some authors is the demonstration of a clonal B-cell population by FC. [5][6][7][8][9][10][11]15 It has been shown repeatedly that FC is a useful tool for evaluating lymphoid FNAB specimens.…”

“…[5][6][7][8][9][10][11]15 The consistent contributing factor to an accurate diagnosis of MZL in FNAB reported by some authors is the demonstration of a clonal B-cell population by FC. [5][6][7][8][9][10][11]15 It has been shown repeatedly that FC is a useful tool for evaluating lymphoid FNAB specimens. [15][16][17][18][19][20][21] All eight MZL specimens diagnosed by Young et al had concurrent FC, although specific details, such as the percentage of B-cells versus T-cells and K:L ratios, were not provided.…”

Fine-Needle Aspiration Biopsy in the Diagnosis and Classification of Primary and Recurrent Lymphoma: A Retrospective Analysis of the Utility of Cytomorphology and Flow Cytometry

“…Cases suspicious or equivocal for lymphoma, which would often comprise a significant portion of cases in practice, were not mentioned, and subclassification was not attempted in this series. Young et al (9) recently reported their experience with 100 FNAB, most of which were combined with results of FCM. Of the 81 cases of confirmed lymphoma, 58 (72%) cases were diagnosed cytologically as positive, 18 (22%) as suspicious, and 5 (6%) as equivocal.…”

Adult T-cell leukemia/lymphoma

“…This has been evidenced by studies showing that nonHodgkin lymphomas can be accurately diagnosed in cytologic specimens utilizing ancillary techniques such as immunocytochemistry and flow cytometry. 4,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] In the REAL classification, ATLL belongs to the category of peripheral T-cell and natural killer cell neoplasms. Although several reports of peripheral Tcell lymphoma have been published in the cytology literature, [33][34][35][36][37] we are aware of only a single cytologic study of T-cell lymphoma in HTLV-1 positive patients.…”