Utility of EST-SNP Markers for Improving Management and Use of Olive Genetic Resources: A Case Study at the Worldwide Olive Germplasm Bank of Córdoba

Belaj, Angjelina; Ninot, A.; Gómez-Gálvez, Francisco J.; Riachy, Milad El; Gurbuz-Veral, Melek; Torres, Mariela; Lazaj, Adhurim; Klepo, Tatjana; Cremades, Javier; Baldoni, Luciana; Lorite, Ignacio J.; Šatović, Zlatko; Rosa, Raúl de la

doi:10.3390/plants11070921

Cited by 23 publications

(50 citation statements)

References 97 publications

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“…The present work includes 50 olive genotypes (40 cultivated varieties and 10 wild accessions) that represent a wide range of fruit weight phenotypes and reflect all the geographic olive distribution areas in the Mediterranean Basin [ 32 , 38 ]. The same genotypes have been previously included in genomic studies [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Identification of Genetic Markers and Genes Putatively Involved in Determining Olive Fruit Weight

Moret

Ramírez-Tejero

Serrano

et al. 2022

Plants

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The fruit size of a cultivated olive tree is consistently larger than its corresponding wild relatives because fruit size is one of the main traits associated with olive tree domestication. Additionally, large fruit size is one of the main objectives of modern olive breeding programs. However, as the long juvenile period is one main hindrance in classic breeding approaches, obtaining genetic markers associated with this trait is a highly desirable tool. For this reason, GWAS analysis of both genetic markers and the genes associated with fruit size determination, measured as fruit weight, was herein carried out in 50 genotypes, of which 40 corresponded to cultivated and 10 to wild olive trees. As a result, 113 genetic markers were identified, which showed a very high statistically significant correlation with fruit weight variability, p < 10−10. These genetic markers corresponded to 39 clusters of genes in linkage disequilibrium. The analysis of a segregating progeny of the cross of “Frantoio” and “Picual” cultivars allowed us to confirm 10 of the 18 analyzed clusters. The annotation of the genes in each cluster and the expression pattern of the samples taken throughout fruit development by RNAseq enabled us to suggest that some studied genes are involved in olive fruit weight determination.

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Section: Methodsmentioning

confidence: 99%

Identification of Genetic Markers and Genes Putatively Involved in Determining Olive Fruit Weight

Moret

Ramírez-Tejero

Serrano

et al. 2022

Plants

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“…In addition, fruit samples from the cultivars “Arbequina”, “Frantoio”, “Picual” and “Koroneki” were also included in the experiment for comparison. The genotype acting as the female parent belongs to the wild olive Germplasm Bank preserved at the Institute of Agricultural and Fishery Research and Training, Córdoba, Spain [ 49 ]. Fruit samples (around 1 kg) were randomly collected for each plant on a common date (mid-October).…”

Section: Methodsmentioning

confidence: 99%

Fruit Phenolic and Triterpenic Composition of Progenies of Olea europaea subsp. cuspidata, an Interesting Phytochemical Source to Be Included in Olive Breeding Programs

Serrano-García

Olmo-García

Polo-Megías

et al. 2022

Plants

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Olea europaea subsp. cuspidata has a relatively low commercial value due to the low size and pulp to stone ratio of its drupes compared to commercial olive cultivars. Nevertheless, this subspecies could represent a valid source of useful traits for olive breeding. In the current work, the drupe metabolic composition (secoiridoids, flavonoids, simple phenols, triterpenic acids, etc.) of a progeny of 27 cuspidata genotypes coming from free pollination and their female parent was evaluated by applying a powerful LC-MS method. A total of 62 compounds were detected within the profiles; 60 of them were annotated and 27 quantified. From a quantitative point of view, the genotypes from the progeny of cuspidata showed quite different metabolic profiles to olive common cultivars (“Arbequina”, “Frantoio”, “Koroneiki” and “Picual”) used as controls. Cuspidata drupes were richer in terms of several bioactive compounds such as rutin, hydroxytyrosol glucoside, a few interesting secoiridoids and the compounds of m/z 421 and 363. The relationships among several secondary metabolites determined in the progeny inferred from the results of both PCA and cross-correlation analysis were explained according to metabolic biosynthesis pathways in olive drupes. These outcomes underlined the potential of cuspidata genetic resources as a source of potentially interesting variability in olive breeding programs.

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“…To address this need, the World Olive Germplasm Bank of Coŕdoba (WOGBC) plays a crucial role by conserving as much olive genetic patrimony as possible. WOGBC is established at the experimental field "Alameda del Obispo" of the Andalusian Institute for Research and Training in Agriculture, Fishery, Food and Organic Production (IFAPA), and represents a reference olive germplasm bank both at national (INIA-ESP046) and international level (IOC) (Belaj et al, 2016;Dıéz et al, 2018;Belaj et al, 2022). The accurate identification of olive material is a crucial task in germplasm banks (Atienza et al, 2013;Trujillo et al, 2014;El Bakkali et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

New insights in the Spanish gene pool of olive (Olea europaea L.) preserved ex situ and in situ based on high-throughput molecular markers

Gómez-Gálvez,

Ninot,

Rodríguez

et al. 2024

Front. Plant Sci.

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In Spain, several local studies have highlighted the likely presence of unknown olive cultivars distinct from the approximately 260 ones previously described in the literature. Furthermore, recent advancements in identification techniques have significantly enhanced in terms of efficacy and precision. This scenario motivated a new nationwide prospecting effort aimed at recovering and characterizing new cultivated germplasm using high-throughput molecular markers. In the present study, the use of 96 EST-SNP markers allowed the identification of a considerable amount of new material (173 new genotypes) coming from areas with low intensification of production in different regions of Spain. As a result, the number of distinct national genotypes documented in the World Olive Germplasm Bank of IFAPA, Córdoba (WOGBC-ESP046) increased to 427. Likewise, 65 and 24 new synonymy and homonymy cases were identified, respectively. This rise in the number of different national cultivars allowed to deepen the knowledge about the underlying genetic structure. The great genetic variability of Spanish germplasm was confirmed, and a new hot spot of diversity was identified in the northern regions of La Rioja and Aragon. Analysis of the genetic structure showed a clear separation between the germplasm of southern and northern-northeastern Spain and indicated a significantly higher level of admixture in the latter. Given the expansion of modern olive cultivation with only a few cultivars, this cryptic germplasm is in great danger of disappearing. This underlines the fact that maintaining as many cultivars as possible will increase the genetic variability of the olive gene pool to meet the future challenges of olive cultivation.

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Utility of EST-SNP Markers for Improving Management and Use of Olive Genetic Resources: A Case Study at the Worldwide Olive Germplasm Bank of Córdoba

Cited by 23 publications

References 97 publications

Identification of Genetic Markers and Genes Putatively Involved in Determining Olive Fruit Weight

Identification of Genetic Markers and Genes Putatively Involved in Determining Olive Fruit Weight

Fruit Phenolic and Triterpenic Composition of Progenies of Olea europaea subsp. cuspidata, an Interesting Phytochemical Source to Be Included in Olive Breeding Programs

New insights in the Spanish gene pool of olive (Olea europaea L.) preserved ex situ and in situ based on high-throughput molecular markers

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