Utility of alkaloids as chemical and biomarkers for quality, efficacy, and safety assessment of botanical ingredients

Osman, Ahmed Galal; Haider, Saqlain; Chittiboyina, Amar G.; Khan, Ikhlas A.

doi:10.1016/j.phymed.2018.03.064

Cited by 16 publications

(11 citation statements)

References 61 publications

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“…To develop chromatographic analytical methods, first, it is necessary to perform a chemical characterization of the samples through isolation and identification of their main compounds. Then, the isolated compounds can be used in pharmacological and toxicological investigation studies to select the chemical markers and active metabolites [13,27,28], and to develop analytical methods for the quality control of natural sources, extracts, and products [22][23][24][25].…”

Section: Resultsmentioning

confidence: 99%

Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high‐performance liquid chromatography method for its analysis

Santos

Oliveira

Ribeiro

et al. 2021

J of Separation Science

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Propolis comprises a complex resinous product composed of plant's parts or exudates, pollen, bee wax, and enzymes. Brazilian brown propolis from Araucaria sp displays several biological activities. Considering the lack of validated analytical methods for its analysis, we are reporting the development of a validated high‐performance liquid chromatography with photodiode array detector method to analyze Araucaria brown propolis. The crude propolis were extracted and chromatographed, furnishing six main diterpenes. The isolated standards were used to draw the analytical curves, allowing the studies of selectivity, precision, accuracy, recovery, robustness, the determination of limits of detection and limits of quantification. The mobile phase consisted of 0.1% acetic acid in water and acetonitrile, using an octadecylsilane column, 1 mL/min flow rate and detection at 200 or 241 nm. Relative standard deviation values obtained for intra‐day and inter‐day precision were lower than 4% for all diterpenes. From the five parameters for robustness, wavelength detection and flow rate were the critical ones. Limits of detection and quantification ranged from 0.808 to 10.359 μg/mL and from 2.448 to 31.392 μg/mL, respectively. The recoveries were between 105.03 and 108.13%, with relative standard deviation values around 5.0%. The developed method is precise, sensitive, and reliable for analyzing Araucaria brown propolis.

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Section: Resultsmentioning

confidence: 99%

Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high‐performance liquid chromatography method for its analysis

Santos

Oliveira

Ribeiro

et al. 2021

J of Separation Science

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“…The precipitate generated because the test used KI produced complex compounds between metal ions and reagents [16]. In agriculture, this alkaloid was a chemical that is used as a parameter in assessed the quality of botanical ingredients [18]. Another function of these alkaloids is to inhibited the grew of bacteria or can be called antibacterial [11].…”

Section: Fig 2 Eco-enzyme Ph Measurementmentioning

confidence: 99%

Production and Characterization of Eco-Enzyme from Fruit Peel Waste

Nurlatifah¹,

Agustine²,

Puspasari³

2022

Proceedings of the 1st International Conference on Social, Science, and Technology, ICSST 2021, 25 November 2021, Tangerang, In

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The amount of household waste such as fruit peel waste which is organic waste will have an impact on the environment. The organic waste can be processed into eco-enzyme. Eco-enzyme is obtained from the fermentation process. The fruit peel used are watermelon, pineapple, banana, and orange. The fermentation process used fruit peel waste, water, and sugar in a ratio of 3 : 10 : 1. After 90 days of the fermentation process, filtering is carried out to obtain the filtrate which is an eco-enzyme. The obtained of eco-enzyme has brown and a sour aroma. Eco-enzyme positive has contains acetic acid and lactic acid, also flavonoids, alkaloids, and saponins. This eco-enzyme can be used to cleaned the floors, as a fertilizer, and others. It has changed what was a waste at the first, into something that has many benefits, by processed the waste in the right way.

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“…Besides their use to verify the authenticity of plant material, the presence of compounds associated with the biological activities are an indicator of the efficacy of a product. In T. sericea root bark, sericoside and sericic acid were previously identified as biomarkers [28,29]. Sericoside is known to possess anti-inflammatory and antiaging activities [30][31][32] while sericic acid is known as an antibacterial agent [20].…”

Section: Table 1 Sample Concentration With Band Intensity (Key: Faint (+) Intense (++) Very Intense (+++))mentioning

confidence: 99%

Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics

et al. 2021

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Terminalia sericea is used throughout Africa for the treatment of a variety of conditions and has been identified as a potential commercial plant. The study was aimed at establishing a high-performance thin layer chromatography (HPTLC) chemical fingerprint for T. sericea root bark as a reference for quality control and exploring chemical variation within the species using HPTLC metabo3lomics. Forty-two root bark samples were collected from ten populations in South Africa and extracted with dichloromethane: methanol (1:1). An HPTLC method was optimized to resolve the major compounds from other sample components. Dichloromethane: ethyl acetate: methanol: formic acid (90:10:30:1) was used as the developing solvent and the plates were visualized using 10% sulfuric acid in methanol as derivatizing agent. The concentrations of three major bioactive compounds, sericic acid, sericoside and resveratrol-3-O-β-rutinoside, in the extracts were determined using a validated ultra-performance liquid chromatography-photodiode array (UPLC-PDA) detection method. The rTLC software (written in the R-programming language) was used to select the most informative retardation factor (Rf) ranges from the images of the analysed sample extracts. Further chemometric models, including principal component analysis (PCA) and hierarchical cluster analysis (HCA), were constructed using the web-based high throughput metabolomic software. The rTLC chemometric models were compared with the models previously obtained from ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS). A characteristic fingerprint containing clear bands for the three bioactive compounds was established. All three bioactive compounds were present in all the samples, although their corresponding band intensities varied. The intensities correlated with the UPLC-PDA results, in that samples containing a high concentration of a particular compound, displayed a more intense band. Chemometric analysis using HCA revealed two chemotypes, and the subsequent construction of a loadings plot indicated that sericic acid and sericoside were responsible for the chemotypic variation; with sericoside concentrated in Chemotype 1, while sericic acid was more abundant in Chemotype 2. A characteristic chemical fingerprint with clearly distinguishable features was established for T. sericea root bark that can be used for species authentication, and to select samples with high concentrations of a particular marker compound(s). Different chemotypes, potentially differing in their therapeutic potency towards a particular target, could be distinguished. The models revealed the three analytes as biomarkers, corresponding to results reported for UPLC-MS profiling and thereby indicating that HPTLC is a suitable technique for the quality control of T. sericea root bark.

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Utility of alkaloids as chemical and biomarkers for quality, efficacy, and safety assessment of botanical ingredients

Cited by 16 publications

References 61 publications

Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high‐performance liquid chromatography method for its analysis

Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high‐performance liquid chromatography method for its analysis

Production and Characterization of Eco-Enzyme from Fruit Peel Waste

Phytochemical Profiling and Quality Control of Terminalia sericea Burch. ex DC. Using HPTLC Metabolomics

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