Utility of a Rapid Fecal Latex Agglutination Test Detecting the Neutrophil Protein, Lactoferrin, for Diagnosing Inflammatory Causes of Chronic Diarrhea

Fine, Kenneth D.; Ogunji, F. O.; George, Jason; Niehaus, Mark D.; Guerrant, Richard L.

doi:10.1111/j.1572-0241.1998.413_l.x

Cited by 59 publications

(17 citation statements)

References 25 publications

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“…Several studies on the differential diagnosis of intestinal inflammatory conditions vs. noninflammatory syndromes evidenced that the fecal lactoferrin is a reliable marker of bowel inflammation with sensitivity of 90 percent and specificity of 98 percent. 9,10,12,13,22 On the other hand, calprotectin is easily measured in feces by commercially available ELISA, and it has extensively been used in CD diagnosis and monitoring. 8,15,16,23,24 None of these studies was performed in a postoperative context.…”

Section: Discussionmentioning

confidence: 99%

Fecal Lactoferrin and Calprotectin After Ileocolonic Resection for Crohn's Disease

Scarpa¹,

D’Incà²,

Basso³

et al. 2007

Diseases of the Colon &Amp; Rectum

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Crohn's disease patients maintain high fecal levels of lactoferrin and calprotectin at long-term follow-up after resection of the diseased bowel even in case of clinical remission. The significant correlation between the two fecal markers may be the expression of the ongoing intestinal inflammation. Only lactoferrin significantly correlated with C-reactive protein and showed a reliable threshold value for systemic inflammation. Lactoferrin fecal levels may be a reliable indicator for intestinal inflammation influencing the systemic inflammatory status. The third predictor of lactoferrin fecal level was the presence of episodes of clinical recurrence during the postoperative follow-up.

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Section: Discussionmentioning

confidence: 99%

Fecal Lactoferrin and Calprotectin After Ileocolonic Resection for Crohn's Disease

Scarpa¹,

D’Incà²,

Basso³

et al. 2007

Diseases of the Colon &Amp; Rectum

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“…Calprotectin is a granulocyte-derived, calciumbinding protein that has been evaluated as a diagnostic assay to identify inflammatory diarrhea [121][122][123] and to quantify "inflammatory activity" [124][125][126]. Similarly, lactoferrin, a neutrophil granulocytic protein quantified by a latex agglutination assay [127] or by a polyclonal antibody-based enzyme linked immunoassay has been evaluated to discriminate between active IBD and irritable bowel syndrome [128].…”

Section: Fecal Markersmentioning

confidence: 99%

Inflammatory Bowel Disease

Stenson

Hanauer

Cohen

2008

Textbook of Gastroenterology

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In the US, H. pylori vacA shows allelic variation in the signal sequence (which may be type sla, slb or s2) and the mid-region (type ml or m2). Previous PCR-based vacA mid-region typing classified most, but not all, Asian and South American strains tested as ml or m2. We now sought to investigate vacA mid-region diversity further.MotlmodL We studied 13 Japanese, 6 Chinese, 9 Thai, and 8 Peruvian H. pylori isolates. cagA was identified by colony hybridisation (CH), vacA signal sequence was typed by PCR, and vacA mid-region was typed proximally by CH and distally by PCR. Sections of the vacA mid-region from 8 strains were PCR-amplified, sequenced, and compared with known sequences from 8 other strains.Rcuts Of the 36 Asian and South American strains studied, 35 were cagA+ (the cagA-was Peruvian) and 35 were vacA sla (1 cagA + Peruvian was slb). vacA mid-regions from the 13 Japanese strains were not PCR-amplified by ml or m2-specific primers, but hybridised weakly with an ml probe. Sequence analysis of vacA from 1 Japanese strain revealed 91% nucleotide identity with the ml probe but only 71% identity with the m2 probe. The previously equivocal Thai and Peruvian strains also had ml-like mid-region sequences. A Chinese strain was ml in the proximal mid-region and m2 distally, showing a clear crossover site. Final mid-region types were: Japanese, all 13 ml; Chinese, 1 ml, 1 ml/m2, 4 m2; Thai, 3 ml, 6 m2; Peruvian 4 ml, 4 m2. Distal mid-region sequences of 16 strains, compared over 294 bp, clustered into 2 groups, ml and m2. Nucleotide identity between ml and m2 strains ranged from 73-78%. Within groups, m2 strains were less diverse than ml strains (m2 range 94-99.7%, ml 88-99.3%, p<0.001). Sequence analysis of 7 ml and 3 m2 strains over 1.1kb proximally showed maintenance of clustering outside the 294bp region C duions These Asian and South American strains are similar in terms of cagA status and vacA sla genotype, but fall into 2 vacA midregion groups. ml sequences are more diverse than m2, and thus may be phylogenetically older. The vacA sequence of 1 Chinese strain suggests recombination in vivo between ml and m2 alleles. Introduction: H.pylori antimicrobial susceptibility is an important determinant ofthe efficacy of eradication therapies'. The prevalence of antimicrobial resistance varies within the UK and may increase given the increased use of eradication therapy. This multicentre study assesses the prevalence and possible associations ofH.pylon antimicrobial resistance. Methods: H.pylon was isolated from antral biopsies of patients undergoing routine endoscopy and cultured according to standard microbiological methods. Antimicrobial resistance was determined using "E-tests" or disc tests (tinidazole only) with breakpoints defined by previous studies. Results: H.pyloni was isolated from 32% (1222/3823) of patients and antimicrobial susceptibility determined in 90% (1077/1222) Previous case-control studies of IBD have used hospital or community derived controls. There are obvious inherent biases in both metho...

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“…1,2,17 Released by activated PMN, its presence is proportional to the neutrophil flux to the gastrointestinal tract. Playing an important role in the innate immunity as a bactericidal agent, 4,5 lactoferrin is a 76-kDa iron-binding glycoprotein that is the main component of the secondary granules of the neutrophils that degranulate during inflammatory processes.…”

Section: Discussionmentioning

confidence: 99%

“…1,2 According to a recent study, fecal lactoferrin is a more sensitive surrogate marker of endoscopic Crohn's disease (CD) activity than is Crohn's Disease activity index (CDAI) or C-reactive protein (CRP), and it is a useful tool in clinical practice to estimate disease activity and to monitor treatment response. 3 The main component of the secondary granules of activated neutrophils that degranulate during inflammatory processes, lactoferrin has an important role in the innate immune system.…”

Section: Introductionmentioning

confidence: 99%

Subclinical Intestinal Inflammation in Patients with Crohn’s Disease Following Bowel Resection: A Smoldering Fire

Ruffolo

Scarpa

Faggian

et al. 2010

Journal of Gastrointestinal Surgery

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Utility of a Rapid Fecal Latex Agglutination Test Detecting the Neutrophil Protein, Lactoferrin, for Diagnosing Inflammatory Causes of Chronic Diarrhea

Abstract: The major cause of fecal neutrophils in patients with chronic diarrhea is chronic inflammatory bowel disease of the colon. The latex agglutination test for fecal lactoferrin offers a highly sensitive, specific, and simple means for detection of fecal neutrophils in these patients.

Cited by 59 publications

References 25 publications

Fecal Lactoferrin and Calprotectin After Ileocolonic Resection for Crohn's Disease

Fecal Lactoferrin and Calprotectin After Ileocolonic Resection for Crohn's Disease

Inflammatory Bowel Disease

Subclinical Intestinal Inflammation in Patients with Crohn’s Disease Following Bowel Resection: A Smoldering Fire

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