Uterine preparation for implantation in the mouse is associated with coordinate expression of estrogen-responsive finger protein and estrogen receptor

Das, Niranjan; Wang, Jue; Dey, Sudhansu K.

doi:10.1002/(sici)1098-2795(199704)46:4<499::aid-mrd8>3.0.co;2-j

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…The opening of the WOI on cycle days 19 to 20 is associated with loss of epithelial estrogen and P receptors in normal women. This has now been shown to be a consistent finding across many mammalian species studied, including mouse, pig, horse, and nonhuman primate (23)(24)(25)(26). Spatial and temporal changes in integrins in endometrium also exhibited alterations in expression during the menstrual cycle that frames the WOI, as it is now defined (27) (Fig.…”

supporting

confidence: 83%

Assessment of endometrial receptivity

Lessey

2011

Fertility and Sterility

214

122

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supporting

confidence: 83%

Assessment of endometrial receptivity

Lessey

2011

Fertility and Sterility

214

122

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“…However, the nature of the interactions between these two steroids which trigger the implantation and decidualization processes remain undefined. The dramatic downregulation of the nuclear estrogen receptor-(ER-) (Das et al 1996) and very low levels of ER-(our unpublished observation) in the deciduum suggest that the participation of nuclear ERs in the process of decidualization is limited (Das et al 1996). This is consistent with the observation that P 4 , not estrogen, is an absolute requirement for sustained decidualization in the rodent.…”

Section: Discussionmentioning

confidence: 82%

Cyclin D3 in the mouse uterus is associated with the decidualization process during early pregnancy

Das

Lim²,

Paria³

et al. 1999

Journal of Molecular Endocrinology

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In the mouse, the attachment reaction between the blastocyst trophectoderm and the receptive uterine luminal epithelium occurs at 2200-2300 h on day 4 of pregnancy and is rapidly followed by transformation of stromal cells into decidual cells (decidual cell reaction). This process can also be induced experimentally (deciduoma) by intraluminal oil infusion in the uterus on day 4 of pseudopregnancy. The decidual cell reaction is associated with up-and down-regulation of many genes in a cell-specific manner. Using mRNA differential display, we identified cyclin D3 as one of the genes that is upregulated in the uterus at the sites of blastocyst apposition during the attachment reaction. The levels of expression were low in the morning of days 1-4 as determined by Northern hybridization. In situ hybridization analysis showed that on days 1 and 2, signals were primarily localized in uterine epithelial cells, while signals were detected in both the stromal and epithelial cells on days 3 and 4. In contrast, with the initiation and progression of decidualization on days 5, 6 and 7, the levels of cyclin D3 mRNA were remarkably upregulated in stromal cells both at the mesometrial and the antimesometrial poles. However, on day 8, signals were primarily localized in stromal cells at the mesometrial decidual bed. Implanting blastocysts on these days also expressed cyclin D3 mRNA. In the progesterone-treated delayed implanting mice, the uterine levels of cyclin D3 mRNA were modest at the sites of blastocyst apposition, but were upregulated with the onset of implantation by estradiol-17 . However, the decidual expression of cyclin D3 mRNA was not dependent on the presence of blastocysts, since increased expression also occurred in experimentally induced deciduoma in the absence of blastocysts. The importance of cyclin D3 in decidualization was further examined in Hoxa-10-deficient mice which show defective decidualization. The expression of cyclin D3 mRNA in Hoxa-10( / ) uteri on day 5 was severely compromised after application of a deciduogenic stimulus on day 4 of pseudopregnancy. Collectively, the results suggest that cyclin D3 could be important for the process of decidualization.

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“…In a previous report, the co-localization of efp and ERa mRNA in mouse ovaries was shown by in situ hybridization [29]. The expression of efp in the periimplantation mouse uterus was also showed by in situ hybridization, which suggested the role of efp in amplifying the estrogen effect in preparing of the uterus for implantation [31]. In the present study efp was detected in mouse ovaries and cumulus-oocyte complexes, a finding compatible with the previous study by Orimo et a1.…”

Section: Discussionmentioning

confidence: 99%

Stage-Specific Expression of Estrogen Receptor Subtypes and Estrogen Responsive Finger Protein in Preimplantational Mouse Embryos.

et al. 1999

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Abstract. In hope of understanding possible roles of estrogen during early embryogenesis, we examined the expression of both estrogen receptor a (ERa) and ER/3, a recently cloned novel subtype, in mouse oocytes and preimplantation embryos by means of reverse transcription polymerase chain reaction (RT-PCR). To investigate whether estrogen actually exerts its action, we further determined the expression of efp (estrogen-responsive finger protein), a newly characterized estrogen responsive gene belonging to the RING finger family. ERa mRNA was detected in whole ovaries, cumulus-oocyte complexes, denuded oocytes, 2-cell and 4-cell embryos, whereas it was undetected in 8-cell embryos. Interestingly it reappeared in morulae and blastocysts.ER/3 mRNA was detected similarly to ERa except for the absence of ERfl mRNA in morulae. The efp mRNA was detected in whole ovaries, cumulus-oocyte complexes, 4-cell embryos, morulae and blastocysts. The stage specific expression of ERa and ERI3 along with detection of the product of the estrogen responsive gene in early preimplantation embryos may indicate the possible physiological roles of estrogen in early embryogenesis.

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Uterine preparation for implantation in the mouse is associated with coordinate expression of estrogen-responsive finger protein and estrogen receptor

Cited by 12 publications

References 18 publications

Assessment of endometrial receptivity

Assessment of endometrial receptivity

Cyclin D3 in the mouse uterus is associated with the decidualization process during early pregnancy

Stage-Specific Expression of Estrogen Receptor Subtypes and Estrogen Responsive Finger Protein in Preimplantational Mouse Embryos.

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