Using type III recombinant human collagen to construct a series of highly porous scaffolds for tissue regeneration

Yang, Yang; Ritchie, Alastair Campbell; Everitt, Nicola M.

doi:10.1016/j.colsurfb.2021.112139

Cited by 17 publications

(23 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative blot quantification detects on average higher collagen III and fibronectin presence in iDFU ECM (nonsignificant); equivalent amounts of collagen I to normal cells; and higher collagen I than DFU. Collagen III is elevated in scarless fetal regeneration, making it desirable for inclusion in skin regeneration scaffolds (Figure S1, Supporting Information) [9a,10c−e,13a,c,15b] . These results are in line with the expectation of having more GAGs and collagen III for reprogrammed iDFU fibroblasts, while DFUs have an impaired ECM production and less collagen III content; as per previous data generated on cell lines, fibroblasts cycled through iPS reprogramming produce a rejuvenated matrix signature [13c,d] …”

Section: Resultssupporting

confidence: 83%

“…Briefly, we have chosen for this feasibility study, one clone (named i2DFU8 previously). ECM elements enriched in these cells include those also found in fetal skin ECM (i.e., collagen III and GAG, giving benefit to the wound healing [ 15 ] ), and these cells had enhanced healing in a human skin equivalent model. We hypothesized that its potential is linked to its extracellular matrix.…”

Section: Introductionmentioning

confidence: 99%

“…We hypothesized that its potential is linked to its extracellular matrix. Following the previous proteomics analysis of the matrix of these rejuvenated fibroblasts, we have chosen seven ECM markers relevant for regeneration to analyze and found increased: [13a,d,16] GAGs, collagen I (main component of ECM [ 17 ] ), collagen III (collagen type found in increased levels during scarless wound healing [ 18 ] ), collagen IV (basal lamina [ 19 ] ), fibronectin 1 (primordial ECM deposited in wound healing, [13c,15c] LAMA1 (present in mature skin [ 20 ] ), and LAMA5 (staminal niche [ 21 ] ). We have analyzed these components and fabricated a porous freeze‐dried scaffold using their ECM and analyzed the potential of this scaffold for diabetic wound repair.…”

Section: Introductionmentioning

confidence: 99%

“…We have analyzed these components and fabricated a porous freeze‐dried scaffold using their ECM and analyzed the potential of this scaffold for diabetic wound repair. To assess our scaffolds in vitro, we chose to monitor key processes impaired in the DFU healing process (e.g., cell proliferation, ECM production, vascularization potential) [13b,15a] . To our knowledge, this is the first‐time patient‐specific iPS cell reprogramming to generate a source ECM for scaffold fabrication has been used for any application for tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem‐Reprogrammed Patient Cells

Santarella

Amaral

Lemoine

et al. 2022

Advanced NanoBiomed Research

View full text Add to dashboard Cite

Diabetic foot ulcers (DFUs) are chronic wounds sustained by pathological fibroblasts and aberrant extracellular matrix (ECM). Porous collagen‐based scaffolds (CS) have shown clinical promise for treating DFUs but may benefit from functional enhancements. Our previous work showed fibroblasts differentiated from induced pluripotent stem cells are an effective source of new ECM mimicking fetal matrix, which notably promotes scar‐free healing. Likewise, functionalizing CS with this rejuvenated ECM shows potential for DFU healing. Herein, an approach to DFU healing is demonstrated for the first time using biopsied cells from DFU patients, reprogramming those cells, and functionalizing CS with patient‐specific ECM as a personalized acellular tissue‐engineered scaffold. A two‐pronged approach is taken: 1) direct ECM blending into scaffold fabrication, and 2) seeding scaffolds with reprogrammed fibroblasts for ECM deposition followed by decellularization. The decellularization approach reduces cell number requirements and maintains naturally deposited ECM proteins. Both approaches show enhanced ECM deposition from DFU fibroblasts. Decellularized scaffolds additionally enhance glycosaminoglycan deposition and subsequent vascularization. Finally, reprogrammed ECM scaffolds from patient‐matched DFU fibroblasts outperform those from healthy fibroblasts in several metrics, suggesting ECM is in fact able to redirect resident pathological fibroblasts in DFUs toward healing, and a patient‐specific ECM signature may be beneficial.

show abstract

Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem‐Reprogrammed Patient Cells

Santarella

Amaral

Lemoine

et al. 2022

Advanced NanoBiomed Research

View full text Add to dashboard Cite

show abstract

“…Subsequently, a high-level expression strain was selected from the transformants for high-cell-density fermentation [30]. The RHC-based biomaterials have been developed into porous scaffolds and hydrogels in our previous studies [31,32]. Therefore, RHC was chosen as the biomaterial to prepare nanofibers.…”

Section: Introductionmentioning

confidence: 99%

Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend

Yang

Deng

2021

Polymers

Self Cite

View full text Add to dashboard Cite

Cardiovascular diseases, including coronary artery and peripheral vascular pathologies, are leading causes of mortality. As an alternative to autografts, prosthetic grafts have been developed to reduce the death rate. This study presents the development and characterization of bilayer vascular grafts with appropriate structural and biocompatibility properties. A polymer blend of recombinant human collagen (RHC) peptides and polycaprolactone (PCL) was used to build the inner layer of the graft by electrospinning and co-electrospinning the water-soluble polyethylene oxide (PEO) as sacrificial material together with PCL to generate the porous outer layer. The mechanical test demonstrated the bilayer scaffold’s appropriate mechanical properties as compared with the native vascular structure. Human umbilical vein endothelial cells (HUVEC) showed enhanced adhesion to the lumen after seeding on nanoscale fibers. Meanwhile, by enhancing the porosity of the microfibrous outer layer through the removal of PEO fibers, rat smooth muscle cells (A7r5) could proliferate and infiltrate the porous layer easily.

show abstract

Ultrafast Self‐Healing, Superstretchable, and Ultra‐Strong Polymer Cluster‐Based Adhesive Based on Aromatic Acid Cross‐Linkers for Excellent Hydrogel Strain Sensors

Chen,

Pang,

et al. 2023

Small

View full text Add to dashboard Cite

Synthetic hydrogel strain sensors rarely exhibit a comprehensive combination of mechanical properties such as ultra‐stretchability, ultrafast self‐healing, and high sensitivity. Herein, seven small molecule enhanced mechanical behaviors of polymer‐cluster based hydrogels are demonstrated. The oxidized polyethyleneimine/polymeric acrylic acid (ohPEI/PAA) hydrogels with aromatic formic acids as supramolecular cross‐linkers are prepared by simultaneous formation of ohPEI polymer clusters and PAA upon the addition of ammonium persulfate. The optimized hydrogel adhesive exhibits comprehensive excellent properties, such as high extensibility (up to 12 298%), real‐time mechanical self‐healing capability (<1 s, 93% efficiency), high uniformity, underwater adhesivity, and water‐sealing ability. The proper binding strength of hydrogel and skin (47 kPa) allows the hydrogel to be utilized as highly sensitive (gauge factor:16.08), highly conductive (2.58 mS cm−1), and underwater strain sensors. Specially, the adhesive strength of the adhesive to wood after dehydration is extremely high, reaching up to 29.59 MPa. Additionally, when glycerol is introduced, the obtained gel maintains the physical properties even at harsh‐temperature conditions (−40 to 80 °C). It presents that multiple and hierarchical non‐covalent interactions including multiple hydrogen bonding interactions, π–π stacking, electrostatic interactions, and dipole–dipole interactions of polymer clusters, allow for the energy dissipation and contribute to the excellent performance of the hydrogel.

show abstract

Using type III recombinant human collagen to construct a series of highly porous scaffolds for tissue regeneration

Cited by 17 publications

References 51 publications

Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem‐Reprogrammed Patient Cells

Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem‐Reprogrammed Patient Cells

Porous Bilayer Vascular Grafts Fabricated from Electrospinning of the Recombinant Human Collagen (RHC) Peptide-Based Blend

Ultrafast Self‐Healing, Superstretchable, and Ultra‐Strong Polymer Cluster‐Based Adhesive Based on Aromatic Acid Cross‐Linkers for Excellent Hydrogel Strain Sensors

Contact Info

Product

Resources

About