Using microRNAs as molecular biomarkers for the evaluation of male infertility

Asadpour, Reza; Chelan, Ehsan Mofidi

doi:10.1111/and.14298

Cited by 8 publications

(5 citation statements)

References 67 publications

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“…Some miRNAs have specific expression patterns inside the testis, either in different somatic cell types or in germ cells [ 21 ]. The presence of miRNAs in mature spermatozoa and seminal plasma and the alterations in their expression profiles in patients with spermatogenic problems demonstrate their potential as biomarkers for diagnosis and classification of male fertility [ 65 , 66 , 67 ]. These molecules represent a tiny function within the total RNA from samples, which can vary significantly across samples, so its normalization in RT-PCR analyses represents a massive challenge.…”

Section: Discussionmentioning

confidence: 99%

Further Insights on RNA Expression and Sperm Motility

Silva

Viana

Barros

et al. 2022

Genes

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Asthenozoospermia is one of the main causes of male infertility and it is characterized by reduced sperm motility. Several mutations in genes that code for structural or functional constituents of the sperm have already been identified as known causes of asthenozoospermia. In contrast, the role of sperm RNA in regulating sperm motility is still not fully understood. Consequently, here we aim to contribute to the knowledge regarding the expression of sperm RNA, and ultimately, to provide further insights into its relationship with sperm motility. We investigated the expression of a group of mRNAs by using real-time PCR (CATSPER3, CFAP44, CRHR1, HIP1, IQCG KRT34, LRRC6, QRICH2, RSPH6A, SPATA33 and TEKT2) and the highest score corresponding to the target miRNA for each mRNA in asthenozoospermic and normozoospermic individuals. We observed a reduced expression of all mRNAs and miRNAs in asthenozoospermic patients compared to controls, with a more accentuated reduction in patients with progressive sperm motility lower than 15%. Our work provides further insights regarding the role of RNA in regulating sperm motility. Further studies are required to determine how these genes and their corresponding miRNA act regarding sperm motility, particularly KRT34 and CRHR1, which have not previously been seen to play a significant role in regulating sperm motility.

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Section: Discussionmentioning

confidence: 99%

Further Insights on RNA Expression and Sperm Motility

Silva

Viana

Barros

et al. 2022

Genes

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“…This explains why each differentiating cell type has its own transcriptome profile [ 30 ]. Several studies have highlighted the role of small RNAs (siRNAs, piRNAs, miRNAs) in spermatogenesis and, therefore, in male fertility (reviewed in [ 6 , 17 , 29 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]). In particular, studies have shown that miRNAs can be expressed exclusively or preferentially in the testis and in specific testicular cell types, as shown by Gan et al, who found miRNAs to be more abundant in spermatogonia than in other testis cell types [ 34 , 39 , 40 , 41 ].…”

Section: The Role Of Mirnas In Spermatogenesismentioning

confidence: 99%

“…Fertility-related issues can lead to alterations in the expression profile of miRNAs, and this dysregulation may be a contributing factor to infertility [ 37 ]. Several studies have reported altered miRNA expression profiles with an infertility phenotype, and, therefore, miRNAs could have the potential to serve as diagnostic biomarkers (reviewed in [ 36 , 37 , 38 , 89 ]). Table 2 summarizes a number of studies that demonstrate the link between miRNA profile and impaired fertility.…”

Section: Mirnas In Male Infertility and Their Use As Biomarkersmentioning

confidence: 99%

The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation?

Klees,

Alexandri,

Demeestere

et al. 2023

IJMS

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Oncological treatments have dramatically improved over the last decade, and as a result, survival rates for cancer patients have also improved. Quality of life, including concerns about fertility, has become a major focus for both oncologists and patients. While oncologic treatments are often highly effective at suppressing neoplastic growth, they are frequently associated with severe gonadotoxicity, leading to infertility. For male patients, the therapeutic option to preserve fertility is semen cryopreservation. In prepubertal patients, immature testicular tissue can be sampled and stored to allow post-cure transplantation of the tissue, immature germ cells, or in vitro spermatogenesis. However, experimental techniques have not yet been proven effective for restoring sperm production for these patients. MicroRNAs (miRNAs) have emerged as promising molecular markers and therapeutic tools in various diseases. These small regulatory RNAs possess the unique characteristic of having multiple gene targets. MiRNA-based therapeutics can, therefore, be used to modulate the expression of different genes involved in signaling pathways dysregulated by changes in the physiological environment (disease, temperature, ex vivo culture, pharmacological agents). This review discusses the possible role of miRNA as an innovative treatment option in male fertility preservation–restoration strategies and describes the diverse applications where these new therapeutic tools could serve as fertility protection agents.

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“…Spermatogenesis is a complex process that can be divided into 3 stages: 1) mitosis, 2) meiosis and 3) spermiogenesis ( 19 ). Defects in this process may lead to the occurrence of NOA, which is one of the causes of male infertility.…”

Section: Sncrnamentioning

confidence: 99%

The gene regulatory role of non-coding RNAs in non-obstructive azoospermia

Zhou

Zhang

et al. 2022

Front. Endocrinol.

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Non-coding RNAs are classified as small non-coding RNAs, long non-coding RNAs and circular RNAs, which are involved in a variety of biological processes, including cell differentiation, proliferation, apoptosis and pathological conditions of various diseases. Many studies have shown that non-coding RNAs are related to spermatogenesis, maturation, apoptosis, function, etc. In addition, the expression of non-coding RNAs in testicular tissue and semen of patients with non-obstructive azoospermia was different. However, the role of non-coding RNAs in the pathogenesis of non-obstructive azoospermia has not been fully elucidated, and the role of non-coding RNAs in non-obstructive azoospermia is rarely reviewed. Here we summarize the research progress of non-coding RNAs in the pathogenesis of non-obstructive azoospermia.

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Using microRNAs as molecular biomarkers for the evaluation of male infertility

Cited by 8 publications

References 67 publications

Further Insights on RNA Expression and Sperm Motility

Further Insights on RNA Expression and Sperm Motility

The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation?

The gene regulatory role of non-coding RNAs in non-obstructive azoospermia

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