Using Microcontact Printing to Pattern the Attachment of Mammalian Cells to Self-Assembled Monolayers of Alkanethiolates on Transparent Films of Gold and Silver

Mrksich, Milan; Dike, Laura E.; Tien, Joe; Ingber, Donald E.; Whitesides, George M.

doi:10.1006/excr.1997.3668

Cited by 462 publications

(374 citation statements)

References 13 publications

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“…235 A range of techniques have been used to micropattern endothelial cells. [236][237][238][239][240][241][242][243] Chen et al 244 found that cell spreading acted as a regulator of proliferation or death in human and bovine capillary endothelial (CE) cells by tightly controlling cell spreading with adhesive microislands of fibronectin; fibronectin was adsorbed on a methyl-terminated alkanethiol SAM, whereas the nonadhesive background consisted of a hexaethylene-glycolterminated alkanethiol SAM (HEG-SAM) that resisted protein adsorption and cell attachment. Cells that were free to spread over large microislands displayed growth, as expected, but the cells that were constrained to limit their spreading to a small island displayed apoptosis.…”

Section: Ve Endothelial Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Ve Endothelial Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…1 Self-assembled monolayers (SAMs) can be engineered to exhibit a variety of chemical properties and reactivities making them hydrophobic, [2][3][4] repulsive, 5,6 or electrochemically active. [7][8][9][10] In practice, SAMs employing biotin have been studied in association with streptavidin conjugated to DNA, proteins, and nanoparticles. [11][12][13][14][15][16] Such SAMs are vital for bioassay technologies such as DNA chips, protein chips, and small molecule biosensors.…”

Section: Introductionmentioning

confidence: 99%

Formation and removal of alkylthiolate self-assembled monolayers on gold in aqueous solutions

Canaria

Maloney

et al. 2006

Lab Chip

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We report the development of novel reagents and approaches for generating recyclable biosensors. The use of aqueous media for the formation of protein binding alkylthiolate monolayers on Au surfaces results in accelerated alkylthiolate monolayer formation and improvement in monolayer integrity as visualized by fluorescence microscopy and CV techniques. We have also developed an electrocleaning protocol that is compatible with microfluidics devices, and this technique serves as an on-chip method for cleaning Au substrates both before and after monolayer formation. The techniques for the formation and dissociation of biotinylated SAMs from aqueous solvents reported here may be applied towards the development of Au-based sensor devices and microfluidics chips in the future. A potential use of these devices includes the specific capture and triggered release of target cells, proteins, or small molecules from liquid samples.

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“…They lifted the cell sheets off the surfaces by lowering the temperature to room temperature, and then successfully applied the cell sheets to repair of corneal and cardiac tissues. Nano-scale fibers are also being applied to the design of tissue engineering scaffolds, an exciting and rapidly expanding field [20][21][22][23][24][25][26][27]. …”

Section: Diagnostic Devicesmentioning

confidence: 99%