Using metabolic charge production in the tricarboxylic acid cycle (QTCA) to evaluate the extracellular-electron-transfer performances of Shewanella spp.

Li, Shiue-Lin; Yen, Jui-Hung; Kano, Kenji; Liu, Shiu‐Mei; Liu, Chien‐Liang; Cheng, Sheng-Shung; Chen, Hsun-Yi

doi:10.1016/j.bioelechem.2018.07.001

Cited by 6 publications

(10 citation statements)

References 47 publications

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“…45 mM organic acids (i.e., lactate and acetate) were added as electron donors. Other details, including the reactor configuration, anolyte and catholyte composition, and temperature- and redox-condition maintenances are given in our previous works (Li et al, 2018). All the experiments in the present study were conducted at 35°C and pH 7.…”

Section: Methodsmentioning

confidence: 99%

“…When carrying out EET on an anode, Shewanella cells catalyze the substrate oxidation and electrode reduction, which also play a role as products (i.e., cell synthesis) of the entire reaction. In our recent study (Li et al, 2018), we developed a mathematic calculation describing cell growth, lactate consumption, as well as pyruvate and acetate production, which is based on the known central metabolic pathways involved in S. oneidensis MR-1 (Scott and Nealson, 1994). According to our previous findings, it is clarified that the intermediate (i.e., pyruvate and acetate) productions can indicate whether the lactate has been partially or completely oxidized, which are the major sources generating nicotinamide-adenine-dinucleotide equivalents to contribute current generation.…”

Section: Methodsmentioning

confidence: 99%

“…This result indicates that Shewanella spp. cannot obtain the necessary adenosine triphosphate (ATPs) via the oxidative phosphorylation at high mediator concentrations (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…While the Shewanella spp. have been intensively applied in many BES studies so far (Li et al, 2010; Zhang et al, 2017; Li et al, 2018), to our best knowledge, no reported study has yet to utilize acetate as an electron donor to drive EET on the Shewanella anodes, although it was reported that Shewanella spp. are able to use acetate to reduce some electron acceptors, such as nitrate, which can be uptaken without the need of EET (Yoon et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Chemical Characteristics of Electron Shuttles Affect Extracellular Electron Transfer: Shewanella decolorationis NTOU1 Simultaneously Exploiting Acetate and Mediators

Wang

Chen

et al. 2019

Front. Microbiol.

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In the present study, we found that our isolate Shewanella decolorationis NTOU1 is able to degrade acetate under anaerobic condition with concomitant implementation of extracellular electron transfer (EET). With +0.63 V (vs. SHE) poised on the anode, in a 72-h experiment digesting acetate, only 2 mM acetate was consumed, which provides 6% of the electron equivalents derived from the initial substrate mass to support biomass (5%) and current generation (1%). To clarify the effects on EET of the addition of electron-shuttles, riboflavin, anthraquinone-2,6-disulfonate (AQDS), hexaammineruthenium, and hexacyanoferrate were selected to be spiked into the electrochemical cell in four individual experiments. It was found that the mediators with proton-associated characteristics (i.e., riboflavin and AQDS) would not enhance current generation, but the metal-complex mediators (i.e., hexaammineruthenium, and hexacyanoferrate) significantly enhanced current generation as the concentration increased. According to the results of electrochemical analyses, the i-V graphs represent that the catalytic current induced by the primitive electron shuttles started at the onset potential of −0.27 V and continued increasing until +0.73 V. In the riboflavin-addition experiment, the catalytic current initiated at the same potential but rapid saturated beyond −0.07 V; this indicated that the addition of riboflavin affects mediator secretion by S. decolorationis NTOU1. It was also found that the current was eliminated after adding 48 mM N-acetyl-L-methionine (i.e., the cytochrome inhibitor) when using acetate as a substrate, indicating the importance of outer-membrane cytochrome.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…This result indicates that Shewanella spp. cannot obtain the necessary adenosine triphosphate (ATPs) via the oxidative phosphorylation at high mediator concentrations (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical Characteristics of Electron Shuttles Affect Extracellular Electron Transfer: Shewanella decolorationis NTOU1 Simultaneously Exploiting Acetate and Mediators

Wang

Chen

et al. 2019

Front. Microbiol.

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“…Asam sianida adalah senyawa kimia yang mengandung gugus siano C ≡ N yang terikat dengan atom hidrogen (7; 10; 26; 27; 32; [41][42][43][44][45] , atom karbon terikat tiga ke atom nitrogen. Pada gugus CN sebagai ion sianida poliatomik yang bermuatan negatif (CN -).…”

Section: Jari-jari Atom Penyusun Hcnunclassified

Analisis Molekular dan Karakteristik Hidrogen Sianida (HCN)

Husna¹,

Zainul²

2019

Preprint

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Hidrogen sianida dalam air membentuk asam lemah bersifat toksik. Tujuan review ini untuk menganalisis molekular dan transpor elektron hidrogen sianida. Metode yang digunakan adalah penggambaran molekul dengan aplikasi Chem Office versi 15.0 , untuk mengetahui transpor ion asam sianida yaitu dengan parameter konduktivitas, viskositas, mobilitas ion, dan gerakan hanyut.Hasil yang didapat yaitu struktur molekul asam sianida pada analisis molekuler 2D, dan bentuk molekul asam sianida dalam 3D. Berdasarkan metode ini diperoleh energi kinetik rotasi senyawa 2,0466 kcal/mol,. Untuk konduktivitas, kecepatan hanyut, gerakan ion,dilakukan dengan menggunakan metode perhitungan dengan rumusan hasilnyadan beberapa soal yang dibuatkan grafiknya Analisis jari-jari atom hidrogen dengan carbon pada Chemdraw 3D didapatkan sebesar 1,1 A, dan jari jari atom carbon dengan nitrogen masing-masingnya sebesar 1,1 A. Data yang didapat pada Analisa MM2 dynamics HCN adalah interaction time 0.024 , 0.026, 0.028, 0.030, total energy 0.048, 0.082, potensial energy 0.38, 0.045, 0.016, 0.091.sifat dari asam sianida diantaranya massa molar 27,03 g/mol, densitas 0,687g/mL, viskositas : 201 μPa s. Sifat termodinamika hidrogen sianida adalah ∆G (enegi gibs) 143,1 kj/mol entropi (∆S) 113,01 J/kmol , entalpi pembentukan standar (∆fH) -141,2 kJ/mol , entalpi pembakaran standar (∆cH) 426,5 kJ/mol dan kecepatan hanyut yang didapat berdasarkan perhitungan sebesar 2,33 V/cm.

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Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide

Zheng

Xiao

et al. 2019

Biosensors and Bioelectronics

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The extracellular electron transfer of Shewanella oneidensis MR-1 (MR-1) has been extensively studied due to the importance of the biosensors and energy applications of bioelectrochemical systems. However, the oxidation of metal compounds by MR-1, which represents the inward extracellular electron transfer from extracellular electron donors into the microbe, is barely understood. In this study, MR-1 immobilized on an electrode electrocatalyzes the oxidation of [Fe(CN) 6] 4to [Fe(CN)6] 3efficiently and selectively. The selectivity depends on midpoint potential and overall charge(s) of redox molecules. Among 12 investigated redox molecules, the negatively charged molecules with high midpoint potentials, i.e., [Ru(CN)6] 4and [Fe(CN)6] 4-, show strong electrocatalysis. Neither reference bacteria (Escherichia coli K-12 nor Streptococcus mutans) electrocatalyze the oxidation of [Fe(CN)6] 4-. The electrocatalysis decays when MR-1 is covered with palladium nanoparticles presumptively involved with cytochromes c. However, cytochromes c MtrC and OmcA on MR-1 do not play an essential role in this process. The results support a model that [Fe(CN)6] 4donor electrons to MR-1 by interacting with undiscovered active sites and the electrons are subsequently transferred to the electrode through the mediating effect of [Fe(CN)6] 4-/3-. The selective electron uptake by MR-1 provides valuable insights into the fundamental insights of the applications of bioelectrochemical systems and the detection of specific redox molecules.

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Using metabolic charge production in the tricarboxylic acid cycle (QTCA) to evaluate the extracellular-electron-transfer performances of Shewanella spp.

Cited by 6 publications

References 47 publications

Chemical Characteristics of Electron Shuttles Affect Extracellular Electron Transfer: Shewanella decolorationis NTOU1 Simultaneously Exploiting Acetate and Mediators

Chemical Characteristics of Electron Shuttles Affect Extracellular Electron Transfer: Shewanella decolorationis NTOU1 Simultaneously Exploiting Acetate and Mediators

Analisis Molekular dan Karakteristik Hidrogen Sianida (HCN)

Electrons selective uptake of a metal-reducing bacterium Shewanella oneidensis MR-1 from ferrocyanide

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