Use of the organotypic EpiVaginal™ tissue model to screen irritation potential of feminine hygiene ingredients and formulations

Kanďárová, Helena; Ayehunie, Seyoum; Cannon, Chris; LaRosa, Karen; Hayden, Patrick; Klausner, Mitchell

doi:10.1016/j.toxlet.2010.03.526

Cited by 1 publication

(4 citation statements)

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“…Existing commercially available in vitro TE vaginal models such as EpiVaginal™ or the HVE models are used in the research community for toxicity and irritability studies of vaginal formulations and understanding the transmission of sexually transmitted infections 9 . These tools are extremely valuable, however, they have several limitations which we aimed to overcome in this study.…”

Section: Discussionmentioning

confidence: 99%

“…SVT, sheep vaginal tissue; TE, tissue engineered infections. 9 These tools are extremely valuable, however, they have several limitations which we aimed to overcome in this study. These models can be prohibitively expensive, have limited culture times, do not include physiologically relevant lamina propria components, and either rely on immortalized cell lines or regular access to human tissue samples.…”

Section: Discussionmentioning

confidence: 99%

“…Later, their model was further developed to produce EpiVaginal™ tissue (VEC-100) and EpiVaginal™ tissue (VEC-100-FT). 8,9 While these models showed morphological similarities with the native human vaginal tissue, but the collagen gel substrate fails to accurately model the complexity of the native lamina propria. Other commercially available models of the human vaginal mucosa include human vaginal epithelium (SkinEthic™ HVE) and reconstructed HVE that uses cancer-derived cell lines to construct the epithelium.…”

Section: Introductionmentioning

confidence: 99%

“…Ayehunie et al 7 first reported the development of a 3D organotypic vaginal‐ectocervical model using human vaginal cells cultured on collagen gel matrix. Later, their model was further developed to produce EpiVaginal™ tissue (VEC‐100) and EpiVaginal™ tissue (VEC‐100‐FT) 8,9 . While these models showed morphological similarities with the native human vaginal tissue, but the collagen gel substrate fails to accurately model the complexity of the native lamina propria.…”

Section: Introductionmentioning

confidence: 99%

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A physiologically relevant, estradiol‐17β [E2]‐responsive in vitro tissue‐engineered model of the vaginal epithelium for vaginal tissue research

Shafaat

Mangır

Chapple

et al. 2022

Neurourology and Urodynamics

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Aims: There are many situations where preclinical models of the human vagina would be valuable for in vitro studies into the pathophysiology of vaginally transmitted diseases, microbicide efficacy, irritability testing, and particularly, for assessing materials to be inserted in the vagina for support of the pelvic floor. The aim of this study is to develop a physiologically relevant, low cost, and ethically suitable model of the vagina using sheep vaginal tissue (SVT) to reduce the need for animal testing in gynecological research.Methods: Tissue-engineered (TE) vaginal models were developed by culturing primary vaginal epithelial cells and vaginal fibroblasts, isolated from the native SVTs on decellularized sheep vaginal matrices at an air-liquid interface.Morphological analyses of the models were conducted by performing hematoxylin and eosin staining and further characterization was done by immunohistofluorescence (IHF) of structural proteins and cytokeratins.Results: Histological analysis of the models revealed a gradual formation of a stratified epithelium on our decellularized matrices and cell metabolic activity remained high for 21 days as measured by the resazurin assay. Our models showed a dose-dependent response to estradiol-17β [E 2 ] with an increase in the vaginal epithelium thickness and cellular proliferation under higher E 2 concentrations (100-400 pg/ml). The physiological relevance of these results was confirmed by the IHF analysis of Ki67, and cytokeratins 10 and 19 expression. Conclusion:In this study, we have developed an estradiol-responsive TE vaginal model that closely mimics the structural and physiological properties of the native SVT.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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