Use of the Drosophila wing spot test in the genotoxicity testing of different herbicides

HERBICIDE GENOTOXICITY REVEALED WITH THE SOMATIC WING SPOT ASSAY OF Drosophila melanogaster

“…Linuron clearly increased the frequency of small and total spots at all concentrations tested in the ST cross while a statistical significant result was obtained only at the highest (1 %) concentration tested in the HB cross. These results could be related to investigations with other types of herbicides performed to test their genotoxicity in the wing spot test: alachlor, atrazine and paraquat were only positive in the ST cross (Torres et al 1992), as well as 2,4,5-trichlorophenoxyacetic acid, maleic hydrazide and glyphosate (Kaya et al 2000b). Recently the phenyl-urea herbicide diuron, chemically related to linuron, has been shown to induce similar results in the wing spot test of Drosophila: a positive dose-response effect in the ST cross while only positive at the highest concentration in the HB cross (Peraza-Vega et al 2016).…”

HERBICIDE GENOTOXICITY REVEALED WITH THE SOMATIC WING SPOT ASSAY OF Drosophila melanogaster

“…Linuron clearly increased the frequency of small and total spots at all concentrations tested in the ST cross while a statistical significant result was obtained only at the highest (1 %) concentration tested in the HB cross. These results could be related to investigations with other types of herbicides performed to test their genotoxicity in the wing spot test: alachlor, atrazine and paraquat were only positive in the ST cross (Torres et al 1992), as well as 2,4,5-trichlorophenoxyacetic acid, maleic hydrazide and glyphosate (Kaya et al 2000b). Recently the phenyl-urea herbicide diuron, chemically related to linuron, has been shown to induce similar results in the wing spot test of Drosophila: a positive dose-response effect in the ST cross while only positive at the highest concentration in the HB cross (Peraza-Vega et al 2016).…”

Emerging Bacterial Resistance and Genotoxicity of Water-Soluble Fractions of Agricultural Soils from the Semiarid Region of Brazil Affected by the Continuous Use of Glyphosate

“…From this review, it could be inferred that glyphosate is less toxic to non-target arthropods, compared to other insecticides such as chlorpyrifos or cypermethrin. As previously demonstrated in the model organism D. melanogaster glyphosate exhibits genotoxic and mutagenic properties, increasing risk of genetic damage in this non-target organisms and inducing abnormalities in their life cycles (Galin et al 2019 ; Kaya et al 2000 ; Muller et al 2021 ; Strilbytska et al 2022 ). In P1 and P2 cases of our study, treated groups of D. melanogaster larvae exposed to WSFs revealed higher DNA damage when compared to control.…”

Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay

“…Kale et al (26) reported that RoundupÒ treatment increased the frequency of sexlinked, recessive lethal mutations in Drosophila. Genotoxic effects of glyphosate were also demonstrated by wing spot test on Drosophila by Kaya et al (22). Furthermore, Bolognesi et al (25) documented DNA-damaging activity by alkaline elution technique and 8-hydroxydeoxyguanosine quantification in the mouse liver and kidney, and also a significant increase in micronucleus frequency in the mouse bone marrow in vivo with both substances.…”

HERBICIDE GENOTOXICITY REVEALED WITH THE SOMATIC WING SPOT ASSAY OF Drosophila melanogaster

“…Linuron clearly increased the frequency of small and total spots at all concentrations tested in the ST cross while a statistical significant result was obtained only at the highest (1 %) concentration tested in the HB cross. These results could be related to investigations with other types of herbicides performed to test their genotoxicity in the wing spot test: alachlor, atrazine and paraquat were only positive in the ST cross (Torres et al 1992), as well as 2,4,5-trichlorophenoxyacetic acid, maleic hydrazide and glyphosate (Kaya et al 2000b). Recently the phenyl-urea herbicide diuron, chemically related to linuron, has been shown to induce similar results in the wing spot test of Drosophila: a positive dose-response effect in the ST cross while only positive at the highest concentration in the HB cross (Peraza-Vega et al 2016).…”

Emerging Bacterial Resistance and Genotoxicity of Water-Soluble Fractions of Agricultural Soils from the Semiarid Region of Brazil Affected by the Continuous Use of Glyphosate

“…From this review, it could be inferred that glyphosate is less toxic to non-target arthropods, compared to other insecticides such as chlorpyrifos or cypermethrin. As previously demonstrated in the model organism D. melanogaster glyphosate exhibits genotoxic and mutagenic properties, increasing risk of genetic damage in this non-target organisms and inducing abnormalities in their life cycles (Galin et al 2019 ; Kaya et al 2000 ; Muller et al 2021 ; Strilbytska et al 2022 ). In P1 and P2 cases of our study, treated groups of D. melanogaster larvae exposed to WSFs revealed higher DNA damage when compared to control.…”

Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay

“…Kale et al (26) reported that RoundupÒ treatment increased the frequency of sexlinked, recessive lethal mutations in Drosophila. Genotoxic effects of glyphosate were also demonstrated by wing spot test on Drosophila by Kaya et al (22). Furthermore, Bolognesi et al (25) documented DNA-damaging activity by alkaline elution technique and 8-hydroxydeoxyguanosine quantification in the mouse liver and kidney, and also a significant increase in micronucleus frequency in the mouse bone marrow in vivo with both substances.…”

HERBICIDE GENOTOXICITY REVEALED WITH THE SOMATIC WING SPOT ASSAY OF Drosophila melanogaster

“…Linuron clearly increased the frequency of small and total spots at all concentrations tested in the ST cross while a statistical significant result was obtained only at the highest (1 %) concentration tested in the HB cross. These results could be related to investigations with other types of herbicides performed to test their genotoxicity in the wing spot test: alachlor, atrazine and paraquat were only positive in the ST cross (Torres et al 1992), as well as 2,4,5-trichlorophenoxyacetic acid, maleic hydrazide and glyphosate (Kaya et al 2000b). Recently the phenyl-urea herbicide diuron, chemically related to linuron, has been shown to induce similar results in the wing spot test of Drosophila: a positive dose-response effect in the ST cross while only positive at the highest concentration in the HB cross (Peraza-Vega et al 2016).…”

Emerging Bacterial Resistance and Genotoxicity of Water-Soluble Fractions of Agricultural Soils from the Semiarid Region of Brazil Affected by the Continuous Use of Glyphosate

“…From this review, it could be inferred that glyphosate is less toxic to non-target arthropods, compared to other insecticides such as chlorpyrifos or cypermethrin. As previously demonstrated in the model organism D. melanogaster glyphosate exhibits genotoxic and mutagenic properties, increasing risk of genetic damage in this non-target organisms and inducing abnormalities in their life cycles (Galin et al 2019 ; Kaya et al 2000 ; Muller et al 2021 ; Strilbytska et al 2022 ). In P1 and P2 cases of our study, treated groups of D. melanogaster larvae exposed to WSFs revealed higher DNA damage when compared to control.…”

Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay

“…Kale et al (26) reported that RoundupÒ treatment increased the frequency of sexlinked, recessive lethal mutations in Drosophila. Genotoxic effects of glyphosate were also demonstrated by wing spot test on Drosophila by Kaya et al (22). Furthermore, Bolognesi et al (25) documented DNA-damaging activity by alkaline elution technique and 8-hydroxydeoxyguanosine quantification in the mouse liver and kidney, and also a significant increase in micronucleus frequency in the mouse bone marrow in vivo with both substances.…”