Use of the adenylate energy charge ratio to measure growth state of natural microbial communities.

Wiebe, W. J.; Bancroft, K. C. C.

doi:10.1073/pnas.72.6.2112

Cited by 76 publications

(35 citation statements)

References 9 publications

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“…First, it is nonspecific and is generally applicable: and second, its response to environmental changes is rapid, from minutes for microorganisms (Wiebe & Bancroft, 1975) to 24 h for a mollusc (Wijsman, 1976). Changes of the A EC have been observed in several studies with perturbations in environmental conditions (salinity, temperature, exposure to hydrocarbons, and lack of oxygen) or with growth state.…”

Section: Energy Statusmentioning

confidence: 99%

Tilapia are able to withstand long‐term exposure to low environmental pH, judged by their energy status, ionic balance and plasma cortisol

Ginneken

Eersel

Balm

et al. 1997

Journal of Fish Biology

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Tilapia Oraochromis mossambicus were exposed to water at pH 4-0 for 37 days. The water was acidified slowly over 6 h enabling the animals to acclimate and preventing damage of the gill epithelium. Additional stressors, e.g. aluminium ions and handling stress, were avoided. No mortality or decreased food consumption was observed during the exposure period. No significant changes were observed between the control and acid exposed groups for the energy rich compounds and related parameters, i.e. Lhe adenylate energy charge, the pool of tota! adenine nucleotides, and the IMP load of white muscle and liver, indicating maintenance of homeostasis. Moreover, there were no significant differences between control groups and acidified groups at 3, 17 and 37 days for plasma sodium, chloride, cortisol and glucose, implying that ionic balance was maintained and that there was no activation of the pituitaryinterrenal axis. It is concluded that tilapia can acclimate to water at pH 4*0 when the acidification rate is slow and additional stressors are avoided. (Ç) 1997 The Fisheries Society of the British Isles

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Section: Energy Statusmentioning

confidence: 99%

Tilapia are able to withstand long‐term exposure to low environmental pH, judged by their energy status, ionic balance and plasma cortisol

Ginneken

Eersel

Balm

et al. 1997

Journal of Fish Biology

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“…In these microcosms, microbial biomass remained sufficiently high to support C. capitata activities. In addition, changes in DNA synthesis in microcosms with aerobic and anaerobic aged detritus followed the AEC ratio which is a relative measure of microbial growth rate (Wiebe and Bancroft, 1975). Therefore, if microbial biomass is high, C. capitata production is not tightly coupled to microbial production.…”

Section: Discussionmentioning

confidence: 99%

Microbial Metabolism and Incorporation by the Polychaete Capitella capitata of Aerobically and Anaerobically Decomposed Detritus

Hanson¹,

Tenore²

1981

Mar. Ecol. Prog. Ser.

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Detritus derived from Spartind rlllerniflord (cordgrass), Grdcilaric, foliilera (red seaweed), and periphyton (mixed algae) was decomposed aerobically and anaerobically for various lengths of time and then fed to the polychaete Cdpilelld capilald in flow-through microcosms. Rates of detrital mineralization [CO, production), microbial respiration (0, consumption) and biomass (adenosine triphosphate and total adenylates, A,), and net incorporation by C. cdpitata varied with detrital source and length of pre-aging. Oxygen consumpt~on per unit microbial biomass (ymoles 1cgA;' d-') increased linearly with age of the detritus: periphytic algal detritus had the highest daily increase of 4 %, followed by G. foliiferd and S. dlterniflora detritus at 1 % and 0.3 % respectively. Metabolic respiratory quotient ( C 4 / O 2 ) , which varied from < 1 to about 5 0 . 1, was a function of detrital source and age; ~t indicated that anaerobic bacteria were important decomposers of detritus. Net incorpordtion rates by C. capitata, microbial biomass, and 0, consumption rates did not dlffer among G. foliifera detritus aged under oxic and anoxic conditions. Rates of CO, production, however, were up to 6 times higher for G. foliifera detritus aged anaerobically. The results suggest that a n a e r o b~c metabolism, which causes a high CO, production, could represent a sign~ficant loss of carbon from benthic food webs.

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“…After grinding the frozen tissue with a mortar and pestle, the powder (about I mg of wet weight) was boiled immediately for 3 min in 1.5 ml of 50 mA/ Tris-HCI buffer, pH 7.75, containing 4 mM EDTA, to extract ATP [ 12], After adding 0.5 ml of ATP-releasing agent (An alytical Luminescence Laboratory, San Diego, Calif., USA), the extract was centrifuged at 2,500 rpm for 10 min, and cooled 4°C. The supernatant was used for the ATP assay, based on a linear bioluminescence response of luciferine-luciferase [ 13], using the Lumiphometer (TD4000, Labo Science, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Restoration of ATP Contents in the Transplanted Liver Closely Relates to Graft Viability in Dogs

Higashi

Takenaka²,

Fukuzawa³

et al. 1989

Eur Surg Res

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Searching for a marker of graft viability, adenosine triphosphate (ATP) content in the graft tissue was measured sequentially during orthotopic liver transplantation (OLT) in 24 adult mongrel dogs. According to the survival period, 12 recipients were devided into two groups: group I – early-death group (n = 6), death within 3 days after OLT and group II – survival group (n = 6), survived more than 7 days after OLT (average 12 days). Serum biochemical findings showed no differences between the two groups except that the prothrombin time on the first postoperative day in group I was significantly longer (p < 0.05). The ATP content ratio (% of initial value) was significantly higher in group II, compared to that of group I (p < 0.05), 94 versus 53% at 10 min after perfusion, 31 versus 17% at just before recirculation, respectively. The ATP content ratio recovered significantly to more than 80% at 10 min after recirculation in group II but only to less than 50% in group I. These results suggest that restoration of ATP content in the liver after graft recirculation reflects the viability of the graft and can serve as an appropriate prognostic marker in cases of OLT.

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Use of the adenylate energy charge ratio to measure growth state of natural microbial communities.

Cited by 76 publications

References 9 publications

Tilapia are able to withstand long‐term exposure to low environmental pH, judged by their energy status, ionic balance and plasma cortisol

Tilapia are able to withstand long‐term exposure to low environmental pH, judged by their energy status, ionic balance and plasma cortisol

Microbial Metabolism and Incorporation by the Polychaete Capitella capitata of Aerobically and Anaerobically Decomposed Detritus

Restoration of ATP Contents in the Transplanted Liver Closely Relates to Graft Viability in Dogs

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