Use of ribotyping and random amplified polymorphic DNA to differentiate isolates of Burkholderia andropogonis

Abstract

Aims: The aim of this study was to determine the genetic diversity among isolates of Burkholderia andropogonis from various host plant species and geographic locations. Methods and Results: Both random amplified polymorphic DNA (RAPD) and ribotyping analyses were used to assess the diversity of B. andropogonis isolates and compare these results with pathogenicity assays carried out on a number of common hosts of the organism. Conclusions: Both RAPD and ribotyping analyses revealed a high level of genetic diversity between isolates of B. andropogonis. Both methods demonstrated a similar clustering of isolates. However, there was no strict correlation between the genetic diversity revealed and the original host, geographic location or pathogenicity of the isolates. Significance and Impact of the Study: This is the first report on the genetic diversity of isolates of B. andropogonis. The great degree of diversity revealed in this study contrasts with the lack of phenotypic diversity within this species.

A new bacterial disease of jojoba (Simmondsia chinensis) caused by Burkholderia andropogonis

“…The results for the jojoba isolates were more‐or‐less consistent with those for isolates from other hosts. There is considerable genetic diversity in this species, and Bagsic‐Opulencia et al . (2001) reported two pathogenicity types in the 29 isolates they studied from a variety of hosts.…”

Bacterial black spot caused by Burkholderia andropogonis on Odontoglossum and intergeneric hybrid orchids

“…The spots enlarged and either the infected leaves dropped from bulbs or the inoculated plants ultimately died. Based on the reaction on corn and white clover, the isolates seemed to be similar to pathogenicity type 1 (Bagsic-Opulencia et al 2001). Although Phalaenopsis sp.…”

Mancha bacteriana em Ruscus sp. causada por Burkholderia andropogonis no Brasil

“…Essa espécie bacteriana apresenta relativa homogeneidade com relação à filogenia e características fenotípicas, mas grande diversidade genética entre isolados, sem que tenha sido observada correlação entre hospedeiro, localização geográfica e patogenicidade (Bagsic et al, 1995;Bagsic-Opulencia et al, 2001). No Brasil, esta bactéria já foi descrita causando danos em primavera (Bougainvillea sp.…”