Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae

Francis, Febe; Sabu, Abdulhameed; Nampoothiri, K. Madhavan; Ramachandran, Subramaniam; Ghosh, Sanjoy; Szakács, George; Pandey, Ashok

doi:10.1016/s1369-703x(02)00192-4

Cited by 314 publications

(152 citation statements)

References 21 publications

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“…Many studies have been carried out to optimize the incubation temperature for T. reesei and A. niger. The combined impact of temperature and moisture content has also been reported in similarly studies using spent brewing grains (SBG), rice bran and soybean hulls supplemented with wheat bran [12,49,50].…”

Section: Page 5 Of 10mentioning

confidence: 69%

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Abdullah

Greetham

Pensupa

et al. 2016

J Fundam Renewable Energy

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This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the MSW substrate and production of cellulase enzymes was optimized for temperature, moisture content, inoculation and period of incubation. The effect of additional minerals, and alternative carbon and nitrogen sources were also examined.Following optimization a cellulase activity of 26.10 ± 3.09 FPU/g could be produced using T. reesei at 30°C with a moisture content of 60% with an inoculums of 0.5 million spores/g and incubation for 168 hours. Addition of extra nitrogen and/or carbon did not improve cellulase accumulation. Acid or alkali pretreatment of MSW led to reduced cellulase production. Crude enzymes produced from MSW by T. reesei were evaluated for their ability to release glucose from MSW. A cellulose hydrolysis yield of 24.7% was achieved, which was close to that obtained using a commercial enzyme. Results demonstrated that MSW can be used as an inexpensive lignocellulosic material for the production of cellulase enzymes. Research has revealed that T. reesei can produce 13.4 FPU/g cellulase activity using water hyacinth [8]; 154.58 FPU/g using sugar cane bagasse [10]; 8.2 FPU/g using oil palm empty fruit bunches [11]; and 1.16 FPU/g using rice bran [12] as substrates. Similarly it has been reported that A. Niger could produce 24 FPU/g cellulase activity using wheat straw as a substrate [13]. Other substrates such as banana peel, rice straw, corn cob residue, rice husk, banana fruit stalk, and coconut coir pith have all being used for cellulase production [14,15]. Optimizing Cellulase Production fromAlthough MSW can contain a high lignocellulosic content, there has been limited research into the use of MSW for cellulase production. Other research demonstrated the possibility of using MSW to produce cellulase via SSF. But by using raw not autoclave MSW [16,17]. In this paper, we report an example of using an industrially processed MSW for cellulase production and then the use of the resulting "cellulase cocktail" for the enzymatic hydrolysis of MSW. Materials and Methods Municipal solid wasteMunicipal solid waste (MSW) was kindly supplied by Wilson Steam Storage Ltd (UK). The MSW had been subjected to commercial steam 15 min, respectively. After autoclaving, samples were neutralized to pH 7.0 using either 1 M NaOH or 1 M H 2 SO 4 as appropriate. The samples were then centrifuged at 5000 rpm (4472 g) for 10 min. The solid fraction (pellet) was rinsed three times by adding distilled water to the pellet and re-centrifuged. The solid fraction was then dried overnight at room temperature. Journal of Fundamentals of Renewable Energy and ApplicationsMicroorganisms: Two filamentous fungi, Trichoderma reesei QM6a and Aspergillus niger N402 were kindly donated by Professor David Archer (University of Nottingham, UK). Procedures for storing and cultivating T. reesei and A. n...

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Section: Page 5 Of 10mentioning

confidence: 69%

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Abdullah

Greetham

Pensupa

et al. 2016

J Fundam Renewable Energy

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“…This is through the collapse of the cellulose pores which may have ultimately impeded enzymatic access somewhat. The superior ethanol yields from the sake-based CBP system (relative to other permutations of fungal species tested) were somewhat unexpected, since A. oryzae is not usually considered a cellulolytic fungal species, being more noted for starch hydrolysis [10]. Semiquantitative analysis of secreted enzyme activities, using the congo red staining method, indicated significant cellulase and xylanase activity in the supernatants generated from 5-days incubation and onwards.…”

Section: Resultsmentioning

confidence: 98%

“…20 % ABV) from the starch component found within rice [9]. A. oryzae is responsible for the secretion of the enzymes (primarily α -amylases and endo-1,4-α -D-glucan glucohydrolase EC 3.2.1.1) that hydrolyse the starch into glucose, which S. cerevisiae then utilises for ethanol production [10]. However A. oryzae has also been shown to secrete lignocellulolytic enzymes (endocellulases and various xylanases) in significant quantities when cultured in media containing lignocellulosic biomass [11].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

et al. 2016

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Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system (A. oryzae and S. cerevisiae NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, 1 t of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by A. oryzae in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however, it requires low energy and water inputs, and hence is worthy of further investigation and optimisation.

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“…In the current study, the variance analysis was determined using Fisher's statistical test. The F-value obtained from the ratio of the mean square due to regression to the mean square due to error indicates the influence (significance) of each control factor on the tested model (FRANCIS et al, 2003).…”

Section: Experimental Design To Study the Extraction Conditions Of γ mentioning

confidence: 99%

Extraction of γ-oryzanol from rice bran

2012

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ABSTRACTγ -oryzanol, a phytochemical, has antioxidant activities and potential health benefits. In this study, we aimed to develop a method for extracting high amounts of γ -oryzanol from rice bran and for verifying its stability in different solvents. Of the 5 methods under investigation, the one that yielded the highest γ -oryzanol content was further improved by using a central composite rotational design (DCCR -2 4 , including 8 tests in axial conditions and 4 central points, totaling 28 trials). The parameters under study were temperature, time, volume, and the ratio of hexane:isopropanol. High γ -oryzanol content (13.98 mg . g -1 ) was obtained by using the following extraction conditions: 40° C, 40 min, and 75 mL hexane:isopropanol (1:3). The limits of detection and quantification of the method were 0.9 µg g -1 and 31 µg g -1 , respectively. The instrumental precision was 0.004%, the repeatability (CV r ) was 9.4%, and the recovery was 111.7 ± 17.7%. γ -oryzanol is more stable in isopropanol than in hexane, in which 100% concentration could be maintained after refrigeration for 72 days.Index terms: Antioxidant, spectrophotometer, stability, phytochemistry. RESUMOγ -orizanol, um fitoquímico, tem atividade antioxidante e benefícios potenciais para saúde. Esse estudo objetivou desenvolver um método para extração de alta quantidade de γ -orizanol de farelo de arroz e verificar sua estabilidade em diferentes solventes. Dentre os cinco métodos estudados, o que teve maior rendimento em γ -orizanol foi melhorado, usando um delineamento composto central rotacional (DCCR -2 4 , incluindo 8 experimentos no ponto axial e 4 no ponto central, totalizando 28 experimentos). As variáveis testadas foram temperatura, tempo, volume e proporção de hexano: isopropanol usados na extração. Elevado conteúdo de γ -orizanol (13,98 mg g -1 ) foi obtido quando as condições de extração foram 40° C, 40 min e 75 mL de hexano:isopropanol (1:3). Os limites de detecção e quantificação do método foram 0,9 µg g -1 e 31,0 µg g -1 , respectivamente. A precisão instrumental foi 0,004%, a repetibilidade (CV r ) 9,4% e a recuperação 111,7 ± 17,7%. γ -orizanol é mais estável em isopropanol do que em hexano, onde 100% da concentração foi mantida após refrigeração por 72 dias.Termos para indexação: Antioxidante, espectrofotômetro, estabilidade, fitoquímica.

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Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae

Cited by 314 publications

References 21 publications

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

Extraction of γ-oryzanol from rice bran

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