Use of oral mucosal cell sheets for accelerated oral surgical wound healing

Roh, Jong–Lyel; Lee, Jaewang; Jang, Hye-Jin; Kim, Eun Hye; Shin, Dong-Ho

doi:10.1002/hed.24968

Cited by 15 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our newly developed 3D cell-sheet model for anticancer drug testing involved embedding a cancer spheroid into an oral mucosal cell sheet. We previously developed an in vitro engineered autologous mucosal cell sheet consisting of oral keratinocytes and plasma fibrin containing oral mucosal fibroblasts 12 - 14 . The mucosal cell sheet promoted wound healing in the oral cavity and skin, with minimal scar formation 13 , 14 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of an in vitro cell-sheet cancer model for chemotherapeutic screening

Lee¹,

Shin²,

Roh³

2018

Theranostics

Self Cite

View full text Add to dashboard Cite

Epithelial cancer grows in vivo in a microenvironment that comprises tumour, stroma, and immune cells. A three-dimensional (3D) culture model might be able to mimic the tumour microenvironment in vivo; therefore, we developed a new 3D epithelial cancer model using in vitro cell-sheet engineering and compared the results of treatment with several chemotherapeutic drugs among the 3D cell-sheet model, spheroid culture, and 2D cell culture.Methods: The cell sheet comprised keratinocytes and a plasma fibrin matrix containing fibroblasts. Cancer spheroids with or without cancer-associated fibroblasts (CAFs) were interposed between the keratinocytes and fibrin layer. Cell growth, viability, and hypoxia were measured using the cell counting kit-8, LIVE/DEAD assay, and propidium iodide and LOX-1 staining. The morphology, invasion, and mRNA and protein expression were compared among the different cell culture models.Results: Enhanced resistance to sorafenib and cisplatin by cancer spheroids and CAFs was more easily observed in the 3D than in the 2D model. Invasion by cancer-CAF spheroids into the fibrin matrix was more clearly observed in the 3D cell sheet. The expansion of viable cancer cells increased in the 3D cell sheet, particularly in those with CAFs, which were significantly inhibited by treatment with 10 μM sorafenib or 20 μM cisplatin (P < 0.05). TGF-β1, N-cadherin, and vimentin mRNA and protein levels were higher in the 3D cell-sheet model.Conclusions: The 3D cell sheet-based cancer model could be applied to in vitro observation of epithelial cancer growth and invasion and to anticancer drug testing.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The 3D cancer cell-sheet model was generated by incorporating a cancer spheroid into an oral mucosal cell sheet using the method described in our previous reports 12 - 14 . This study was approved by the institutional review board, and written informed consent was obtained from all participants.…”

Section: Methodsmentioning

confidence: 99%

Development of an in vitro cell-sheet cancer model for chemotherapeutic screening

Lee¹,

Shin²,

Roh³

2018

Theranostics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The methods related to the isolation and in vitro culture of oral mucosal cells were the same as mentioned in our previous reports . The in vitro engineering and in vivo testing of the oral mucosa equivalent is summarized in Figure .…”

Section: Methodsmentioning

confidence: 99%

“…The cultured mucosal graft has been successfully used against large intraoral defects . We have previously developed three‐dimensional oral mucosa equivalent comprising oral keratinocytes and plasma fibrin containing oral mucosal fibroblasts using animal‐product‐free and completely autologous materials . The in vivo efficacy of the in vitro‐ engineered mucosa equivalent was proven from its ability to promote oral surgical wound healing .…”

Section: Introductionmentioning

confidence: 99%

“…We have previously developed three‐dimensional oral mucosa equivalent comprising oral keratinocytes and plasma fibrin containing oral mucosal fibroblasts using animal‐product‐free and completely autologous materials . The in vivo efficacy of the in vitro‐ engineered mucosa equivalent was proven from its ability to promote oral surgical wound healing . We further showed that the oral mucosa equivalents may have the necessary tissue plasticity to promote rapid and scarless cutaneous wound healing and exhibit in vivo effects equivalent to those of skin‐derived equivalents …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Treatment of intractable oral ulceration with an oral mucosa equivalent

2018

Self Cite

View full text Add to dashboard Cite

The current use of steroids or pharmacological immunomodulators for the treatment of intractable oral ulceration is ineffective, necessitating newer cell-based therapeutic approaches. We examined the potential efficacy of an oral mucosa equivalent developed in this study in an in vivo model of repeat major oral ulceration mimicking the intractable oral ulceration observed clinically. Oral mucosal samples and plasma fibrin were obtained from Sprague-Dawley rats. The oral mucosa equivalents were prepared with cultured mucosal keratinocytes and plasma fibrin mixed with cultured fibroblasts. Ulcers were chemically induced on the rat buccal mucosa thrice in 3 weeks and covered with or without mucosa equivalents. Gross and microscopic findings and mRNA expression levels were compared between the ulcer control and mucosa equivalent groups. Oral mucosal keratinocytes and fibroblasts were cultured in vitro to achieve high viability and colony-forming efficiency. The equivalents showed epithelial and subepithelial structures similar to those of oral mucosa and exhibited high p63 positivity. In the in vivo study, ulceration was resolved earlier without significant granulation or scarring in the equivalent group than in control group (p < 0.05). Microscopic examinations revealed rapid re-epithelialization and less fibrosis in the equivalent group than in the control group (p < 0.05). Mucosa equivalentcovered ulcers showed histological characteristics similar to those of the normal buccal mucosa and exhibited lower expression of TGFB1, ACTA2, and FN1 mRNAs than the control group. The in vitro-engineered oral mucosa equivalent promotes ulcer healing without scarring and functional deficits.

show abstract

Engineering Heterogeneous Tumor Models for Biomedical Applications

Wu,

Huang,

Wang

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Tumor tissue engineering holds great promise for replicating the physiological and behavioral characteristics of tumors in vitro. Advances in this field have led to new opportunities for studying the tumor microenvironment and exploring potential anti‐cancer therapeutics. However, the main obstacle to the widespread adoption of tumor models is the poor understanding and insufficient reconstruction of tumor heterogeneity. In this review, the current progress of engineering heterogeneous tumor models is discussed. First, the major components of tumor heterogeneity are summarized, which encompasses various signaling pathways, cell proliferations, and spatial configurations. Then, contemporary approaches are elucidated in tumor engineering that are guided by fundamental principles of tumor biology, and the potential of a bottom‐up approach in tumor engineering is highlighted. Additionally, the characterization approaches and biomedical applications of tumor models are discussed, emphasizing the significant role of engineered tumor models in scientific research and clinical trials. Lastly, the challenges of heterogeneous tumor models in promoting oncology research and tumor therapy are described and key directions for future research are provided.

show abstract

Use of oral mucosal cell sheets for accelerated oral surgical wound healing

Abstract: Autologous cell sheets can be engineered in vitro from oral keratinocytes, fibroblasts, and fibrin, and can be used clinically to accelerate healing of oral soft tissue defects.

Cited by 15 publications

References 24 publications

Development of an in vitro cell-sheet cancer model for chemotherapeutic screening

Development of an in vitro cell-sheet cancer model for chemotherapeutic screening

Treatment of intractable oral ulceration with an oral mucosa equivalent

Engineering Heterogeneous Tumor Models for Biomedical Applications

Contact Info

Product

Resources

About