Vineyards in Nemea, the most important viticultural zone in Greece, were surveyed for indigenous non-Saccharomyces (NS) yeasts of enological potential. NS populations were isolated from the final stage of alcoholic fermentation and identified by a range of molecular methods. The enological profiles of Hanseniaspora guilliermondii, H. osmophila, Lachancea thermotolerans, Starmerella bacillaris and Torulaspora delbrueckii strains were evaluated. Significant interspecies variation was observed in fermentation kinetics. H. osmophila and T. delbrueckii showed the highest capacity for prompt initiation of fermentation, while S. bacillaris achieved a higher fermentation rate in the second half of the process. Significant differences were also observed in the chemical parameters of NS strains. S. bacillaris SbS42 and T. delbrueckii TdS45 were further evaluated in mixed-culture fermentations with Saccharomyces cerevisiae. NS strains achieved lower population densities than S. cerevisiae. SbS42 exhibited a higher death rate than TdS45. The chemical profiles of different ferments were separated by principal component analysis (PCA). Both NS strains were associated with lower levels of ethanol, when compared to single S. cerevisiae inoculation. TdS45 increased the ethyl acetate levels, while SbS42 caused a different production pattern of higher alcohols. This is the first report to explore the enological potential of NS wine yeast populations from Nemea. Based on prominent enological traits identified, the selected S. bacillaris and T. delbrueckii strains may be further exploited as co-culture starters for improving the quality and enhancing the regional character of local wines.Although NS yeasts initiate fermentation, most of them are not detectable at the end of the course, either because they are ethanol-intolerant or incapable of withstanding microbial antagonism [8,9]. However, in various later studies it has been shown that several NS species, such as I. occidentalis, L. thermotolerans, M. pulcherrima, Starmerella bacillaris (synonym C. zemplinina) and Z. bailii, were able to maintain high viable populations (up to 6-7 log CFU/mL) for longer periods than previously thought and were even isolated from the final stages of the alcoholic fermentation [4,7,10,11]. These species may play a crucial role in winemaking, due to the production of important metabolites that shape the flavor and contribute to the style of wines [1,12]. These metabolites include both "volatile" compounds, like aldehydes and esters, and "non-volatile" compounds, like glycerol, acetic acid and lactic acid.In this context, there is a growing interest in the use of well-selected NS yeasts such as Torulaspora, Candida, Metschnikowia and Lachancea/Kluyveromyces species, which when combined with S. cerevisiae in mixed-culture starters can improve wine fermentation and final product quality [13]. Several NS strains have been produced commercially as active dry yeasts and novel starter cultures are continuously developing to address specific challenges in m...