Use of Mineral Oil in the Maintenance of Cultures of Fungi Pathogenic for Humans

Ajello, Libero; Grant, Virginia Q.; Gutzke, Mark A.

doi:10.1001/archderm.1951.01570060075006

Cited by 23 publications

(7 citation statements)

References 2 publications

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“…Cultures of Candida spp., including C. albicans, C. guilliermondii, C. parapsilosis, C. stellatoidea, and C. tropicalis, can be preserved for many months when covered with mineral oil and left at room temperature. Viability of some cultures, which do not survive cold storage, can be maintained by this method (4). A few investigators, including Fennell et al (49) and Wickerham and Andreason (207), recommend lyophilization as the method of choice for preservation of certain fungi and yeast, especially C. albicans.…”

Section: Preservation Of Culturesmentioning

confidence: 99%

A Review of the Genus Candida

Skinner¹,

Fletcher²

1960

Bacteriological Reviews

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Section: Preservation Of Culturesmentioning

confidence: 99%

A Review of the Genus Candida

Skinner¹,

Fletcher²

1960

Bacteriological Reviews

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“…This is in accordance with a study by Smith D et al 7 which preserved 58 strains of fungi and 47 strains that were still viable after being stored for 20 years in paraffin oil. Similarly, Ajello et al 6 also preserved 34 strains of fungi in paraffin oil and all strains that were still viable after being stored for 19-21 months.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, these twopreservation methods could be done in a simple laboratory. [5][6][7][8] With an increasing number of antifungal resistances of many unusual and rare fungi, studies examining the susceptibility profile of fungi are now widely conducted. Most of the source of the fungi is culture collection since strains should be collected over years to reach an amount sufficient to be studied.…”

Section: Introductionmentioning

confidence: 99%

Preservation in Paraffin Oil: Alternative for Fungi Preservation in Simple Laboratory Concept

Surja¹,

Sally²,

Hartoyo³

et al. 2020

amj

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Background: A good collection of fungi culture can be maintained with good preservation methods. With appropriate methods, preservation can be performed in low-budget laboratories, such as with periodic subculture and preservation in either sterile water or in paraffin oil. The aim of this study was to assess the viability, morphology, contamination, and antifungal susceptibility profile of fungi preserved in sterile water or paraffin oil compared to a periodic subculture. Methods: This study was an experimental study using Aspergillus sp., Trichophyton mentagrophytes and Candida albicans. The fungi were preserved by a periodic subculture or in sterile water or in paraffin oil for six months. A comparison of viability, morphology, contamination, and antifungal susceptibility profile between methods were made before and after six months of study. Results: The viability of all the fungi was maintained in both periodic subculture and preservation with paraffin oil. However, the T. mentagrophytes was not viable in preservation using sterile water. All methods could maintain the morphology without contamination. There were reductions in the inhibition zone against antifungal drug in preservation with sterile water and paraffin oil. Conclusion: Preservation in paraffin oil is superior to preservation in sterile water in terms of maintaining fungal viability. This method is suitable for the use in a simple laboratory, albeit considerations should be given to the reduction of inhibition zone.

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“…At present, it may be said that a simple, reliable, and more or less general method for preservation of fungi is not yet established. It has been reported that some long-term preservation of cul-tures did not cause pleomorphism in dermatophytes (2,3) and that maintenance under mineral oil caused fluffy degeneration and reduced sporulation activity (4,5).…”

Section: Discussionmentioning

confidence: 99%

Examination of Some Properties of Dermatophytes for Long‐term Preservation Using Periodic Transfer Method

Kinbara

Mashimo

1983

The Journal of Dermatology

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Seventy‐two strains from species of dermatophytes which had been transferred periodically on Sabouraud's dextrose agar for more than ten years were examined for their morphology, physiological activities and biological properties. Of 42 strains of Trichophyton rubrum, 17 (40.4%) were positive for pigment production on Sabouraud's dextrose agar. In mating experiments, 15 (79%) of 19 strains tested were compatible with Arthroderma simii and proved to be of the “—” mating type. Eight (67%) of 12 strains in the Microsporum gypseum complex were positive for macroconidium formation. In mating experiments, 4 of 12 strains proved to be Nannizzia gypsea of the “+” type, 3 to be of the “—” type, and one to be N. incurvata of the “—” type. Most of the 18 strains in the T. mentagrophytes complex and M. canis have been maintained with alterations.

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Use of Mineral Oil in the Maintenance of Cultures of Fungi Pathogenic for Humans

Cited by 23 publications

References 2 publications

A Review of the Genus Candida

A Review of the Genus Candida

Preservation in Paraffin Oil: Alternative for Fungi Preservation in Simple Laboratory Concept

Examination of Some Properties of Dermatophytes for Long‐term Preservation Using Periodic Transfer Method

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