Use of Mice as Experimental Animals in Laboratories That Refer to the Principles of Animal Welfare:  a Literature Review

Mutiarahmi, Citra Nur; Hartady, Tyagita; Lesmana, Ronny

doi:10.19087/imv.2020.10.1.134

Cited by 18 publications

(19 citation statements)

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“…Salah satu bentuk penelitian eksperimen indoor (penelitian laboratorium) adalah penelitian menggunakan hewan coba. Dua hewan coba yang sering digunakan sebagai sampel penelitian laboratorium adalah mencit dan tikus putih (Mutiarahmi et al, 2021).…”

Section: Pendahuluanunclassified

Pengembangan Pelet Berbasis Nabati sebagai Pakan Alternatif untuk Intervensi Diet Vegetarian pada Tikus Putih (Rattus norvegicus)

Laeto¹,

Diba²,

Inggarsih³

et al. 2023

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Penelitian eksperimen yang menggunakan intervensi diet pada tikus putih (Rattus norvegicus) terus mengalami peningkatan. Bentuk penelitian intervensi diet yang saat ini sering dilakukan adalah diet vegetarian. Penggunaan pelet standar dalam penelitian yang menerapkan diet telah banyak dilakukan, namun pengembangan pelet berbasis nabati masih belum dilakukan di Indonesia. Tujuan penelitian ini adalah untuk menganalisis efektivitas pelet nabati yang dikembangkan melalui pengukuran bobot tubuh tikus putih. Metode yang diterapkan dalam penelitian ini adalah deskriptif dengan desain eksperimen laboratorium. Jumlah pelet nabati yang dikembangkan adalah tiga, yaitu pelet untuk diet vegetarian tipe quasi, lacto-ovo dan vegan. Hasil penelitian menunjukkan bahwa pada kandungan proksimat pelet yang dianalisis, persentase karbohidrat tertinggi ditemukan pada pelet diet vegetarian tipe quasi. Kandungan persentase protein dan lemak tertinggi masing-masing ditemukan pada pelet diet vegan dan quasi. Sedangkan pelet diet lacto-ovo menunjukkan persentase kandungan air yang paling tinggi. Hasil intervensi ketiga bentuk pelet yang diberikan pada tikus putih menunjukkan penambahan bobot tubuh setiap minggu penelitian. Hasil juga menunjukkan bahwa pelet untuk tipe diet lacto-ovo memberi pengaruh yang paling tinggi terhadap peningkatan bobot tubuh tikus putih dibandingkan pelet diet lainnya. Pelet berbasis nabati efektif digunakan sebagai pakan alternatif dalam penelitian intervensi diet vegetarian pada tikus putih.

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Section: Pendahuluanunclassified

Pengembangan Pelet Berbasis Nabati sebagai Pakan Alternatif untuk Intervensi Diet Vegetarian pada Tikus Putih (Rattus norvegicus)

Laeto¹,

Diba²,

Inggarsih³

et al. 2023

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“…Hewan uji yang digunakan pada penelitian ini adalah mencit jantan (Mus musculus) galur Swiss Webster. Penggunaan hewan ini sebagai hewan uji karena selain mudah ditangani, struktur anatomi, fisiologi serta genetiknya mirip dengan manusia khususnya saluran pencernaan seperti lambung, usus halus, sekum dan kolon (Hugenholtz & de Vos, 2018;Mutiarahmi et al, 2021).…”

Section: Hasil Dan Pembahasanunclassified

Uji Efektivitas Antidiare Obat Herbal X pada Mencit (Mus musculus) galur Swiss Webster yang diinduksi Minyak Jarak (Oleum ricini)

Nuraprilia,

Erliana Zahara,

Riadi

et al. 2023

jharma

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Drug X is one of the herbal medicines used as anti-diarrhea that is known to have been circulating in the community. This medicine contains extracts of pomegranate peel, areca nut seeds, gambir leaves, and guava leaves. Its use is only based on empirical data, so further studies are still needed to prove its effectiveness as an antidiarrheal. The purpose of this study was to determine the effectiveness of herbal medicine X as an anti-diarrhea agent in male mice (Mus musculus) induced by castor oil (Oleum ricini). Testing the anti-diarrhea effect was carried out using the method of protection against diarrhea induced by castor oil. Mice were randomly divided into five groups (n = 15). Mice in the normal group were only given the preparation carrier, namely Na-CMC suspension, and were not induced by castor oil. Mice in the positive control group were given Diapet® suspension at a dose of 156 mg/kg BW, while the negative control mice were given Na-CMC suspension, and then both groups were induced with castor oil. Mice in both treatment groups were given X herbal medicine with different doses (128.7 mg/kg BW and 386.1 mg/kg BW) and then induced by castor oil. Diarrhea parameters, including the frequency of defecation and stool consistency, were observed and then statistically analyzed using the ANOVA test, Kruskal-Wallis, and Mann-Whitney. The results showed that the treatment dose of 128.7 mg/kg had comparable antidiarrheal effectiveness with the positive control (p≤0.05) and was significantly different from the negative control (p≤0.05). The dose of 386.1 mg/kg showed no significant difference with the negative control (p≤0.05) and showed a significant difference with the dose of 128.7 mg/kg and the positive control. From this study, it can be concluded that herbal medicine X has an anti-diarrheal effect and that a dose of 128.7 mg/kg BW shows the best anti-diarrheal effect.

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“…Bakteri ekoenzim mampu menguraikan senyawa organik kompleks baik yang mengandung unsur C, H, dan N menjadi senyawa yang lebih sederhana karena mampu menghasilkan enzim ekstraseluler (Basitoh et al, 2018). Hal tersebut sejalan dengan pernyataan Mutiarahmi et al, (2021) yang menyatakan bahwa bakteri menggunakan nutrisi yang terdapat pada lingkungan hidupnya melalui berbagai aktivitas biokimia. Pati atau amilum merupakan salah satu zat organik yang terdapat di dalam ekoenzim.…”

Section: Uji Aktivitas Enzim Amilase Secara Kualitatifunclassified

Bakteri Penghasil Amilase Yang Diisolasi Dari Ekoenzim Limbah Buah-Buahan

Wibowo¹,

Darwis²,

Sipriyadi³

et al. 2022

Biosilampari

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Many of potential enzymes can be found in the ecoenzyme, one of them is amylase. Amylase is an enzyme that is able to hydrolize the glycoside bonds of starch or starch into dextrin, glucose and maltose which is widely used in various industries such as beverages. This study aims to isolate the ecoenzyme bacteria and test their ability to produce amylase. Isolation of ecoenzyme bacteria was carried out using serial dilution methods 10-1, 10-3, 10-5. A total of 0.1 ml of each dilution series was pipetted and spread on the Nutrient agar (NA) medium. The bacterial isolates that grew were then purified and identified by morphological observation, Gram staining and their enzymatic activity was tested using Starch Agar (SA) media qualitatively. The results of this study showed there were 39 bacterial isolates from ecoenzyme with different morphological characteristics. The amylase activity test were found that 34 isolates had a positive activity to hydrolize the starch in the SA media which was indicated by the formation of a clear zone around the bacterial colonies. Each bacterial isolate had a different hydrolysis index value, which ranged from 9.45 to 23.65. The highest clear zone diameter index value from starch hydrolysis was EJM 15 isolate.

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Use of Mice as Experimental Animals in Laboratories That Refer to the Principles of Animal Welfare: a Literature Review

Cited by 18 publications

References 0 publications

Pengembangan Pelet Berbasis Nabati sebagai Pakan Alternatif untuk Intervensi Diet Vegetarian pada Tikus Putih (Rattus norvegicus)

Pengembangan Pelet Berbasis Nabati sebagai Pakan Alternatif untuk Intervensi Diet Vegetarian pada Tikus Putih (Rattus norvegicus)

Uji Efektivitas Antidiare Obat Herbal X pada Mencit (Mus musculus) galur Swiss Webster yang diinduksi Minyak Jarak (Oleum ricini)

Bakteri Penghasil Amilase Yang Diisolasi Dari Ekoenzim Limbah Buah-Buahan

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