Use of mass spectrometry techniques for the characterization of metal bound to proteins (metallomics) in biological systems

Gómez-Ariza, José Luis; García‐Barrera, Tamara; Lorenzo, F.; Bernal, Vicente; Villegas, M.J.; Oliveira, V.

doi:10.1016/j.aca.2004.01.061

Cited by 77 publications

(52 citation statements)

References 71 publications

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“…Such data may help to define mathematical models that can eliminate the potential overestimation of Mg by means of LA-ICP-MS. Similar influences of organic proteins on chemical estimates are also expected for other metals that are often bound to organic molecules such as Co, Fe, Mn, Mo, Ni, Se and Zn (e.g., Lochmüller et al, 1974;Gómez-Ariza et al, 2004). Further studies should therefore isolate different organic components of the shell and determine their amount and chemical composition by wet chemical analyses.…”

Section: Discussionmentioning

confidence: 99%

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Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Schöne¹,

Zhang²,

Jacob³

et al. 2010

Geochem. J.

107

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The element chemistry of biogenic carbonates can provide important data on past environments. However, the Sr/Ca and Mg/Ca ratios as well as the Mg and Sr concentrations of biological carbonates, especially aragonitic bivalves often depart from apparent thermodynamic equilibrium. When measured in situ by means of LA-ICP-MS, the Mg concentration is often substantially enriched (two-to threefold) near the organic-rich, annual growth lines. To test the hypothesis that some organic components exert a major influence on the skeletal metal content, the element chemistry of different shell components (insoluble organic matrix, IOM; dissolved CaCO 3 and soluble organics, SOM) of Arctica islandica was measured by means of ICP-OES and LA-ICP-MS. The ICP-OES data indicate that the IOM is strongly enriched in Mg (130 ppm) and depleted in Sr and Ca (10 ppm and 0.22 wt%, respectively) when compared to the whole biomineral (Mg: 68 to 99 ppm, Sr: 860 to 1,060 ppm, Ca: ~35.72 wt%). Although the average relative abundance of the IOM barely exceeds 0.46 wt%, its chemical composition in combination with its heterogeneous distribution across the shell can significantly increase estimates of the Mg concentration if measured in situ by LA-ICP-MS. Depending on the distribution of the IOM, the Ca concentration may be significantly lower locally than the average Ca concentration of the whole shell (35.72 wt%). If this remains undetected, the Mg concentration of shell portions with higher than average IOM content is overestimated by LA-ICP-MS and, conversely, the Mg concentration is underestimated in shell portions with lower than average IOM content. Removal of the IOM prior to the chemical analysis by LA-ICP-MS or mathematical correction for the IOMderived magnesium concentrations is therefore strongly advised. The different chemistry of the IOM may also exert a major control on the trace element to calcium ratios. Shell portions enriched in IOM will show up to 200 times higher Mg/ Ca and up to two times higher Sr/Ca ratios than the average shell of A. islandica. Without removal of the IOM prior to the analysis, Mg/Ca and Sr/Ca ratios of shell portions with higher IOM content cannot be used as paleothermometers. Because it is currently impossible to remove the IOM prior to chemical analyses by LA-ICP-MS, we recommend the use of wet chemical techniques (= possibility to separate and measure individual shell components) such as ICP-OES at the expense of lower sampling resolution. The results of this study will significantly improve our understanding of shellbased climate and environmental proxies.

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Section: Discussionmentioning

confidence: 99%

“…with magnesium-binding capacities (Gómez-Ariza et al, 2004;Gotliv et al, 2005). Actually, Mg is one of the most abundant cofactors of metalloproteins (Dudev and Lim, 2000) and occurs at negatively charged sites of aspartic and glutamic-rich polypeptides (Dudev et al, 1999).…”

Section: Chemical Composition Of the Insoluble Organic Matrixmentioning

confidence: 99%

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Schöne¹,

Zhang²,

Jacob³

et al. 2010

Geochem. J.

107

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“…O estudo da metalômica das diferentes espécies de peixes poderá fornecer informações valiosas sobre como um íon metálico está distribuído e coordenado às proteínas, como também da concentração individual da espécie metálica, contribuindo assim na elucidação dos aspectos fisiológicos e funcionais dessas biomoléculas. [10][11][12][13] Recentemente foram publicados por pesquisadores brasileiros os primeiros resultados obtidos no estudo metaloproteômico da espécie de peixe tilápia do Nilo. 14,15 Nessas publicações, os autores, utilizando 2D-PAGE no fracionamento das proteínas de plasma e tecido hepático, identificaram, por SR XRF, manganês e zinco em 10 proteínas do plasma e cálcio, ferro e zinco em 26 proteínas do fígado.…”

Section: Introductionunclassified

Fracionamento de cobre em proteínas do plasma, músculo e fígado de tilápia do Nilo

Neves¹,

Lima²,

Baldassini³

et al. 2012

Quím. Nova

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Recebido em 18/4/11; aceito em 7/9/11; publicado na web em 3/11/11 COPPER FRACTIONATION IN PROTEINS FROM PLASMA, MUSCLE AND LIVER OF NILE TILAPIA. Copper fractionation in plasma, muscle and liver of Nile tilapia was performed after protein separation by 2D-PAGE. SR XRF analysis indicated the presence of copper in three protein spots of plasma, and in two protein spots of muscle and liver, respectively. Copper ions were found to be distributed mostly in proteins that had a molar mass of less than 54 kDa and greater than 13 kDa and a pI in the 5.3-9.3 range. The copper concentration bound to these proteins was determined by GFAAS which showed concentrations in the 1.20-4.82 mg g -1 range.Keywords: Nile tilapia; metalloproteins; metallomics. INTRODUÇÃONa nutrição de peixes, como de outros animais, o cobre é elemento dietético essencial. Tanto a deficiência quanto o excesso desse mineral podem causar alterações nas funções fisiológicas e na resposta imunológica, aumentando a susceptibilidade do animal a infecções.1,2 Esse mineral possui importantes funções no organismo dos peixes, sendo necessário no metabolismo de vários nutrientes e como constituinte de diversas enzimas. Destaca-se como referência de sua importância a participação como cofator, dentre outras, da enzima ALA-desidrogenase, a qual participa na síntese do heme em sua incorporação no pigmento hemoglobina. Além disso, é indiretamente responsável também pela formação do colágeno e da elastina, e pela conversão da tirosina em melanina em destruição de ânions superóxido, que são espécies reativas do oxigênio, potencialmente destrutivos para as células. [1][2][3][4] A importância dos minerais como componentes estruturais e funcionais dos organismos vivos e o fato de grande parte das proteínas e enzimas requererem a presença de um íon metálico para realizar alguma atividade biológica levaram os pesquisadores que trabalham com aquicultura a desenvolverem trabalhos relacionados à influência das metaloproteínas na fisiologia, genética e nutrição de peixes. [4][5][6][7][8][9] Nesse contexto, atualmente, o desenvolvimento de metodologias analíticas que permitam uma avaliação segura das metaloproteínas e/ou proteínas ligadas a metais presentes no sangue e em tecidos de animais se tornou fundamental. Principalmente, em relação aos chamados microminerais, como o cobre, os quais apresentam faixas de essencialidade e toxicidade muito próximas e que são transportados em nível celular por diferentes proteínas. Considerando a importância que essas biomoléculas exercem sobre as atividades biológicas dos seres vivos, uma nova área científica denominada metalômica foi proposta recentemente e permitiu a integração de estudos tradicionalmente analíticos com estudos inorgânicos e bioquímicos. O estudo da metalômica das diferentes espécies de peixes poderá fornecer informações valiosas sobre como um íon metálico está distribuído e coordenado às proteínas, como também da concentração individual da espécie metálica, contribuindo assim na elucidação dos aspectos fisiológicos e fun...

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“…[306][307][308] This is especially true for the characterization of metal containing or metal-binding proteins in biological systems (see the new Royal Society of Chemistry journal-Metallomics) and for protein modifications involving 'heteroelements' such as protein phosphorylation. 309,310 Concerning metals, the contribution from spectral interferences is strongly dependent on the matrix (for instance in case of V, Cr, Cu and Zn). Elements such Fe, Mn and Se are always spectrally interfered.…”

Section: Speciation Analysismentioning

confidence: 99%

Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry : Part II. Applications

Jakubowski

Prohaska

Vanhaecke

et al. 2011

J. Anal. At. Spectrom.

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Use of mass spectrometry techniques for the characterization of metal bound to proteins (metallomics) in biological systems

Cited by 77 publications

References 71 publications

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Fracionamento de cobre em proteínas do plasma, músculo e fígado de tilápia do Nilo

Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry : Part II. Applications

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Use of mass spectrometry techniques for the characterization of metal bound to proteins (metallomics) in biological systems

Cited by 77 publications

References 71 publications

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)&mdash;Comparison of ICP-OES and LA-ICP-MS data

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)&mdash;Comparison of ICP-OES and LA-ICP-MS data

Fracionamento de cobre em proteínas do plasma, músculo e fígado de tilápia do Nilo

Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry : Part II. Applications

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Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data

Effect of organic matrices on the determination of the trace element chemistry (Mg, Sr, Mg/Ca, Sr/Ca) of aragonitic bivalve shells (<i>Arctica islandica</i>)—Comparison of ICP-OES and LA-ICP-MS data