Among all alternate fuel sources available, the biodiesel of Jatropha curcas appears to be a potential fuel as it widely exists in Asia. The Jatropha methyl ester (JME) is produced by transesterification process. In the transesterification process, alkyl group of ester exchanges with that of an alcohol. This work focuses on optimizing various parameters like quantities of Methanol, KOH and stirrer speed, which influence high JME yield.Based on L 9 orthogonal array, experiments are conducted by using Taguchi technique.All the nine combinations of L 9 orthogonal array are experimentally done for extraction of JME and then it is used for analysis in Taguchi technique. The analysis of maximum yield is done on the basis of "larger is better" Signal to Noise Ratio (S/N Ratio). The best combination in the L9 orthogonal array is found to be methanol (110 ml), KOH (1.306 gm) and stirrer speed (1200 rpm). The influence of methanol, KOH and stirrer speed on the yield is found by Analysis of Variance (ANOVA). Based on the results of ANOVA, it Downloaded by [University of Nebraska, Lincoln] at 08:45 05 October 2015 2 is found that the influence of Methanol, KOH and Stirrer speed on the yield are obtained as 70.10%, 20.35% and 5.78% respectively.