Use of ion trap gas chromatography–tandem mass spectrometry for detection and confirmation of anabolic substances at trace levels in doping analysis

Elimination kinetic of 17β-estradiol 3-benzoate and 17β-nandrolone laureate ester metabolites in calves’ urine

“…For screening and confirmation of anabolic steroids, gas chromatography coupled to mass spectrometry (GC-MS) is the technique commonly used for the analysis of urine samples by anti-doping or analytical laboratories [2][3][4][5]. Improved analytical sensitivity, leading to increased periods of detection post drug administration, can be achieved in urine using gas chromatography-tandem mass spectrometry (GC-MS/MS) [6][7][8][9] or liquid chromatography-tandem mass spectrometry (LC-MS/MS) [10][11][12][13][14][15]. Efficient control of the illegal use of anabolic steroids must both take into account metabolic patterns and associated kinetics of elimination [5,8,16].…”

Testing for nandrolone metabolites in urine samples of professional athletes and sedentary subjects by GC/MS/MS analysis

“…Because of the high sensitivity of the m/z = 405 signal, this ion was chosen for quantification and for MS-MS analysis. The collision-induced dissociation (CID) process for this analysis was by non-resonant excitation chosen because more structural information can be obtained (Muñoz-Guerra et al, 1997). Optimal CID conditions were evaluated using the automatic method development (AMD) feature that allowed different parameters to be applied during each successive scan.…”

Détection par GC-MS-MS dans les phanères de la prise illégale d'anabolisants stéroïdiens chez l'animal de production et chez les sportifs

“…L'utilisation d'une CPG-SM-SM à trappe d'ions a permis de détecter les traces de 23 stéroïdes (27). Cette technique très sensible permet de détecter et de confirmer des cas positifs qui n'auraient pu l'être par des méthodes de CPG-SM de routine.…”