Use of gum arabic to improve the fabrication of chitosan–gelatin-based nanofibers for tissue engineering

Tsai, Ruei-Yi; Kuo, Ting-Yun; Hung, Shih‐Chieh; Lin, Che-Min; Hsien, Tzu-Yang; Wang, Da-Ming; Hsieh, Hsun-Ping

doi:10.1016/j.carbpol.2014.08.108

Cited by 52 publications

(27 citation statements)

References 33 publications

(24 reference statements)

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“…8, 10 and 12 wt% PVA solutions were prepared and designated as P8, P10 and P12, respectively. The preparation of chitosan solutions suitable for electrospinning was carried out by using our newly developed method [17][18][19]. Our method included the addition of a small amount of gum arabic (10 wt% of chitosan) that significantly decreased the viscosity of chitosan solutions when using a mild aqueous acetic acid solution as a solvent.…”

Section: Preparation Of Solutionsmentioning

confidence: 99%

“…It should be noted that without the addition of gum arabic in chitosan solution, the viscosity of chitosan solution dramatically increased when the concentration of chitosan solution increased to 8 wt% (hollow symbols in Figure 1(b)). Therefore, the preparation of high-content (10-12 wt%) chitosan solutions would be impossible unless using our newly developed method, i.e., the addition of a small amount of gum arabic (10 wt% of chitosan) to greatly decrease the viscosity of chitosan solutions (solid symbols in Figure 1(b)) [17,18]. Gum arabic is a branched biopolymer.…”

Section: Properties Of Pva and Chitosan Solutionsmentioning

confidence: 99%

“…However, TFA and HFIP are highly corrosive and expensive. To prevent the use of TFA and HFIP, we employed our newly developed method (i.e., the addition of gum arabic) to prepare a solution with a high chitosan content but a much lower viscosity, thus making the electrospinning process feasible [17,18]. Gum arabic is a branched biopolymer.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and application of coaxial polyvinyl alcohol/chitosan nanofiber membranes

et al. 2017

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It is difficult to fabricate chitosan-wrapped coaxial nanofibers, because highly viscous chitosan solutions might hinder the manufacturing process. To overcome this difficulty, our newly developed method, which included the addition of a small amount of gum arabic, was utilized to prepare much less viscous chitosan solutions. In this way, coaxial polyvinyl alcohol (PVA)/chitosan (as core/shell) nanofiber membranes were fabricated successfully by coaxial electrospinning. The core/shell structures were confirmed by TEM, and the existence of PVA and chitosan was also verified using FT-IR and TGA. The tensile strength of the nanofiber membranes was increased from 0.6-0.7 MPa to 0.8-0.9 MPa after being crosslinked with glutaraldehyde. The application potential of the PVA/chitosan nanofiber membranes was tested in drug release experiments by loading the core (PVA) with theophylline as a model drug. The use of the coaxial PVA/chitosan nanofiber membranes in drug release extended the release time of theophylline from 5 minutes to 24 hours. Further, the release mechanisms could be described by the Korsmeyer-Peppas model. In summary, by combining the advantages of PVA and chitosan (good mechanical strength and good biocompatibility respectively), the coaxial PVA/chitosan nanofiber membranes are potential biomaterials for various biomedical applications.

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Section: Preparation Of Solutionsmentioning

confidence: 99%

Section: Properties Of Pva and Chitosan Solutionsmentioning

confidence: 99%

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Fabrication and application of coaxial polyvinyl alcohol/chitosan nanofiber membranes

et al. 2017

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“…These copolymers have been studied and reported for various applications. Chitosan has also been used to prepare scaffolds, films, nanoparticles, microparticles, hydrogels, and nanofibers that can be used for various biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of chitosan/gallic acid 3D microporous scaffold for tissue engineering applications

2015

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This study explores the potential of gallic acid incorporated chitosan (CS/GA) 3D scaffolds for tissue engineering applications. Scaffolds were prepared by freezing and lyophilization technique and characterized. FTIR spectra confirmed the presence of GA in chitosan (CS) gel. DSC and TGA analysis revealed that the structure of chitosan was not altered due to the incorporation of GA, but thermal stability was significantly increased compared to the CS scaffold. SEM micrographs showed smooth, homogeneous, and microporous architecture of the scaffolds with good interconnectivity. CS/GA scaffolds exhibited approximately 90% porosity on average, increased swelling (600-900%) and controlled biodegradation (15-40%) in PBS (pH 7.4 at 37°C) with 1 mg/mL of lysozyme. CS/GA scaffolds showed 2-4 fold decrease in CFUs (p < 0.05) for both gram positive and gram negative bacteria compared to the CS scaffold. Cytotoxicity of these scaffolds was evaluated using NIH 3T3 L1 fibroblast cells. CS/GA 0.25% scaffold showed similar viability with CS scaffold at 24 and 48 h. CS/GA scaffolds (0.5-1.0%) showed 60-75% viability at 24 h and 90% at 48 h. SEM images showed that an increased cell attachment was observed for CS/GA scaffolds compared to CS scaffolds. These findings authenticate that CS/GA scaffolds were cytocompatible and would be useful for tissue engineering applications.

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“…Gum Arabic is a natural anionic polysaccharide with highly branched polysaccharide consisting of a β-(1-3) galactose backbone with linked branches of arabinose, rhamnose, and glucuronic acid. The carboxyl groups (of glucoronic acid) are responsible for the negative charge of the gum Arabic above pH 2.2 [25,26]. The glucoronic acid binds the protonated amine group, leading to crosslinking and molecular shrinkage.…”

Section: Differences Between Nano-chitosan Formulations In the Particmentioning

confidence: 99%

Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth

Nugraheni

Soeriyadi

Ustadi

et al. 2019

J. Eng. Technol. Sci.

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Chitosan is known as an antibacterial agent. The effective surface area ratio of chitosan can be increased by modification into nanoparticles. Nanochitosan can be prepared with several simple methods, i.e. precipitation, ionic gelation, or the polyelectrolyte complex method. This study compared these three methods in terms of the targeted product characteristics, i.e. stability of the average nanoparticle size as well as the colloidal dispersion, and the antibacterial characteristics. All three methods resulted in nanoparticle formation, but in the precipitation method significant zeta potential reduction was observed due to the presence of negative ions from the alkali that neutralized the chitosan amine group. The ionic gelation method yielded higher zeta potential and higher inhibition of bacterial growth than those yielded by the polyelectrolyte complex method. Ionic gelation and the polyelectrolyte complex method resulted in much better colloidal dispersion stability than the precipitation method, where a significant particle size increase was observed after one week of storage. This result indicates that both ionic gelation and the polyelectrolyte complex method can be used for forming nano-chitosan for the purpose of food preservation. However, for fishery products it is advisable to use the polyelectrolyte complex method because the TPP usually used in ionic gelation is not allowed to be applied to fish.

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Use of gum arabic to improve the fabrication of chitosan–gelatin-based nanofibers for tissue engineering

Cited by 52 publications

References 33 publications

Fabrication and application of coaxial polyvinyl alcohol/chitosan nanofiber membranes

Fabrication and application of coaxial polyvinyl alcohol/chitosan nanofiber membranes

Fabrication of chitosan/gallic acid 3D microporous scaffold for tissue engineering applications

Comparison of Formulation Methods to Produce Nano-Chitosan as Inhibitor Agent for Bacterial Growth

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