Use of FTIR spectroscopy and PCA-LDC analysis to identify cancerous lesions within the human colon

Kaznowska, Ewa; Depciuch, Joanna; Szmuc, K.; Cebulski, J.

doi:10.1016/j.jpba.2016.11.047

Cited by 52 publications

(35 citation statements)

References 33 publications

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“…In addition, the absorption peaks of amide II that appear at 1560 cm −1 are generated by C–N stretching and amide III at 1316 cm −1 assigned to C–H bend [36]. For the spectrum of shrimp shell, the amide band at 1658 cm −1 is not clearly separated because of the overlapping of peaks of protein [37]. In contrast, an amide band was separated after DESs treatment, indicating that the proteins were eliminated from the shrimp shells.…”

Section: Resultsmentioning

confidence: 99%

Two-Step Separation of Chitin from Shrimp Shells Using Citric Acid and Deep Eutectic Solvents with the Assistance of Microwave

et al. 2019

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In this research, a two-step extraction approach was developed for chitin preparation from shrimp shells by utilizing citric acids and deep eutectic solvents (DESs), which effectively removed minerals and proteins. In the first step, minerals of shrimp shells were removed by citric acid, and the demineralization efficiency reached more than 98%. In the second step, the removal of protein was carried out using deep eutectic solvents with the assistance of microwave, and the deproteinization efficiency was more than 88%. The results of scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction analysis (XRD), and thermogravimetric analysis (TGA) showed that the quality of DES-prepared chitin was comparable to that of traditional acid/alkali-prepared chitin. These results were realized without utilizing hazardous chemicals, which are detrimental to the environment. This research indicates that a DES-based preparation approach has the potential for application in the recovery of biopolymers from natural resources.

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Section: Resultsmentioning

confidence: 99%

Two-Step Separation of Chitin from Shrimp Shells Using Citric Acid and Deep Eutectic Solvents with the Assistance of Microwave

et al. 2019

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“…Several studies have shown the application of multivariate statistical analysis in combination with FTIR spectroscopy to identify and differentiate between cancerous and noncancerous cells [21]. It has been applied to study different types of human and animal cancers such as breast [22], colon [23], prostate [24], brain [25], and cervical [26] cancer. Notably, FTIR analyses have been applied in breast tissue sections to investigate effects on extracellular matrix [27] and fibroblasts [28], to characterize different types of lymphocytes [29], and to distinguish most breast cancer cell lines grown in vitro after formalin-fixed paraffin-embedded (FFPE) tissue processing [30] or in spheroids [31].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy

et al. 2019

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Interactions between programmed death-1 (PD-1) with its ligand PD-L1 on tumor cells can antagonize T cell responses. Inhibiting these interactions using immune checkpoint inhibitors has shown promise in cancer immunotherapy. MDA-MB-231 is a triple negative breast cancer cell line that expresses PD-L1. In this study, we investigated the biochemical changes in MDA-MB-231 cells following treatment with atezolizumab, a specific PD-L1 blocker. Our readouts were Fourier Transform Infrared (FTIR) spectroscopy and flow cytometric analyses. Chemometrical analysis, such as principal component analysis (PCA), was applied to delineate the spectral differences. We were able to identify the chemical alterations in both protein and lipid structure of the treated cells. We found that there was a shift from random coil and α-helical structure to β-sheet conformation of PD-L1 on tumor cells due to atezolizumab treatment, which could hinder binding with its receptors on immune cells, ensuring sustained T cell activation for potent immune responses. This work provides novel information about the effects of atezolizumab at molecular and cellular levels. FTIR bio-spectroscopy, in combination with chemometric analyses, may expedite research and offer new approaches for cancer immunology.

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“…The suitability of the IR spectroscopic technique for the examination of biological samples to objectively characterise the present components and their distribution has been shown in previous studies, most commonly using Fourier transform IR (FTIR) spectroscopic imaging . It has extensively been used to observe prostate , cervix , breast , colon , thyroid , and skin tissues . The most commonly used IR range for analysis of a biological sample is the fingerprint region of 1800 to 900 cm −1 where many molecules exhibit characteristic absorption bands, for instance, amide I band is present between 1690 and 1600 cm −1 ; amide II band is between 1580 and 1480 cm −1 ; and spectral bands of DNA were between 1250 and 1080 cm −1 .…”

Section: Introductionmentioning

confidence: 99%

Thermal effect on dispersive infrared spectroscopic imaging of prostate cancer tissue

Song

Ryu

Morikawa

et al. 2018

Journal of Biophotonics

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A system that combines dispersive infrared micro-spectroscopic imaging and thermography has been developed to study the effect of thermal radiation on the infrared absorption spectra of prostate biopsy samples. The system allows the distribution of thermal signal intensity as a function of emissivity to be interpreted from the integrated absorbance obtained by spectroscopic imaging. Biochemical differences between cancer and benign areas within the specimens are identified in the spectra. Side-by-side comparison of H&E stained adjacent tissue sections with infrared images constructed before and after the removal of thermal effect showed that the latter strongly support differentiation of regions within tissues. The use of spectral bands at discrete wavelengths significantly reduced spectral acquisition time, making this technique promising as a future clinical diagnostic tool. A systemic methodology was implemented to process the data, first by k-means clustering on the second derivative spectra without a priori knowledge, followed by principal component analysis (PCA). Four distinct regions within the tissue samples were successfully classified based on the antisymmetric stretching mode of the methylene functional group. Separation between data in clusters occurs when projecting spectra on a PCA score plot on a plane made by first 2 principal components. The significance of the disparity was verified with statistical test. Regulation of signal to chopper and detector enables simultaneous acquisition of infrared and thermal images of the prostate biopsy tissues.

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Use of FTIR spectroscopy and PCA-LDC analysis to identify cancerous lesions within the human colon

Cited by 52 publications

References 33 publications

Two-Step Separation of Chitin from Shrimp Shells Using Citric Acid and Deep Eutectic Solvents with the Assistance of Microwave

Two-Step Separation of Chitin from Shrimp Shells Using Citric Acid and Deep Eutectic Solvents with the Assistance of Microwave

Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy

Thermal effect on dispersive infrared spectroscopic imaging of prostate cancer tissue

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