Use of Blood and Blood Products

Hunt, Elaine; Moore, Jennifer

doi:10.1016/s0749-0720(15)30900-2

Cited by 14 publications

(14 citation statements)

References 8 publications

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“…The volume of whole blood collected was the recommend for large animals [8,9]. Trichotomy and asepsis of the jugular region was performed for the blood collection.…”

Section: Methodsmentioning

confidence: 99%

Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion

et al. 2012

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BackgroundThis study aimed to evaluate the clinical, haematological and biochemical responses to autologous blood transfusion and the feasibility of this practice in sheep. Thus, we used eight male, 8 months old sheep, weighing on average 30 kg, from which 15 mL/kg of whole blood was collected and stored in CPDA-1 bags. Blood samples were refrigerated for 8 days and subsequently re-infused. The clinical, haematological and biochemical parameters were evaluated before blood collection and reinfusion, after 10 minutes of collection and reinfusion, after 3, 6, 12, 24, 48, 96 and 192 hours after collection and reinfusion.ResultsWith respect to clinical parameters, we observed a decrease in heart rate after 24, 48 and 196 hours from reinfusion compared to basal values (p < 0.05). Haematological variables including globular volume and erythrocyte counts showed a significant decrease (p < 0.01) at all time points after collection and increased (p < 0.01) at all time points after reinfusion. There was a significant increase in total protein and calcium at all time points after reinfusion (p < 0.05).ConclusionAutologous transfusion in sheep slightly altered the physiological, biochemical and haematological responses of sheep, indicating that the technique proposed is safe and can be applied in the clinical practice of this species. The 8 d period was not sufficient for complete recovery of the haematological parameters after blood collection.

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“…The volume of whole blood collected was the recommend for large animals [8,9]. Trichotomy and asepsis of the jugular region was performed for the blood collection.…”

Section: Methodsmentioning

confidence: 99%

Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion

et al. 2012

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“…Post-transfusion reactions are defined as any adverse effect after receipt of total blood or blood components [ 1 ]. Hunt and Moore [ 7 ] considered that the commonest signs of transfusion reactions in ruminants are tachycardia, tachypnea, sudoresis, tremors, fever, pruritus, dyspnea, hematuria, and hemoglobinuria, among others. However, some reports claim that transfusion reactions in ruminants receiving a single blood transfusion are uncommon, as these animals have low levels of circulating isoantibodies [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Clinical Response and Transfusion Reactions of Sheep Subjected to Single Homologous Blood Transfusion

Sousa

Minervino

Araújo

et al. 2014

The Scientific World Journal

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Studies in relation to blood conservation and responses to transfusion are scarce for ruminants. We evaluated the clinical manifestations of sheep that received a single homologous transfusion of whole blood, focusing on transfusion reactions. Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively. Clinical observations were recorded throughout the transfusion, whereas heart rate, respiratory rate, and rectal temperature were assessed at the following times: 24 hours after phlebotomy and before transfusion; 30 minutes, six, twelve, 24, 48, 72, and 96 hours and eight and 16 days after transfusion. All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals). Tachycardia occurred most frequently in the G35 animals (50% of them). During transfusion G35 animals presented more clinical manifestation (P < 0.05). Transfusion of fresh or stored total blood improved the blood volume, but transfusion reactions occurred, demonstrating that a single transfusion of fresh or stored blood can cause inflammatory and febrile nonhemolytic transfusion reactions in sheep.

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“…Although the blood-group system in cattle is highly complex and exceeds the complexity of the human blood-group system [48][49][50], adverse effects after blood transfusion are generally rare [38,39,51]. This is due to the fact that bovine red blood cells do not agglutinate easily, and the important factors in transfusion reactions are haemolysins.…”

Section: Discussionmentioning

confidence: 99%

Absence of classical and atypical (H- and L-) BSE infectivity in the blood of bovines in the clinical end stage of disease as confirmed by intraspecies blood transfusion

Balkema‐Buschmann

Ziegler

Priemer

et al. 2021

Journal of General Virology

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While the presence of bovine spongiform encephalopathy (BSE) infectivity in the blood of clinically affected sheep has been proven by intraspecies blood-transfusion experiments, this question has remained open in the case of BSE-affected cattle. Although the absence of infectivity can be anticipated from the restriction of the agent to neuronal tissues in this species, evidence for this was still lacking. This particularly concerns the production and use of medicinal products and other applications containing bovine blood or preparations thereof. We therefore performed a blood-transfusion experiment from cattle in the clinical end stage of disease after experimental challenge with either classical (C-BSE) or atypical (H- and l-) BSE into calves at 4–6 months of age. The animals were kept in a free-ranging group for 10 years. Starting from 24 months post-transfusion, a thorough clinical examination was performed every 6 weeks in order to detect early symptoms of a BSE infection. Throughout the experiment, the clinical picture of all animals gave no indication of a BSE infection. Upon necropsy, the brainstem samples were analysed by BSE rapid test as well as by the highly sensitive Protein Misfolding Cyclic Amplification (PMCA), all with negative results. These results add resilient data to confirm the absence of BSE infectivity in the donor blood collected from C-, H- and l-BSE-affected cattle even in the final clinical phase of the disease. This finding has important implications for the risk assessment of bovine blood and blood products in the production of medicinal products and other preparations.

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Use of Blood and Blood Products

Cited by 14 publications

References 8 publications

Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion

Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion

Clinical Response and Transfusion Reactions of Sheep Subjected to Single Homologous Blood Transfusion

Absence of classical and atypical (H- and L-) BSE infectivity in the blood of bovines in the clinical end stage of disease as confirmed by intraspecies blood transfusion

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