Use of blocking reagent to confirm enzyme immunoassay results in Chlamydial conjunctivitis

Mårdh, Per‐Anders; Elbagir, Adil Norein; Stenberg, K

doi:10.1007/bf01968065

Cited by 14 publications

(7 citation statements)

References 3 publications

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“…Cervical samples were also tested for C. trachomatis by an enzyme immunoassay (ELISA). Assumed positive ELISA results were also tested by a confirmatory test [13]. The methods used for detection and typing of human papillomavirus (HPV) in cervical cell samples has been described in detail elsewhere [12].…”

Section: Methodsmentioning

confidence: 99%

Vaginale Flora und venerische Erkrankungen bei Frauen mit rezidivierender oder akuter vulvovaginaler Kandidose

et al. 1995

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A history of recurrent vulvovaginal candidiasis (RVVC) was reported by 102 women, while current vulvovaginal candidiasis (VVC) was diagnosed in 83 of the same 996 women. They had all attended two family planning and one youth clinic, respectively. Two women, without RVVC or VVC, matched for age for each case of RVVC, were selected as a comparison group (COMP). Recurrent, but not current VVC, was associated with a history of sexually transmitted disease. Those with current, but not with recurrent, VVC had significantly more often genital warts and bacteriuria (> 10(5) bacteria/ml), but significantly less often bacterial vaginosis than the COMP women. Both VVC and RVVC were inversely correlated to a vaginal flora change with a mixed anaerobic vaginal flora. Those with VVC had a greater number of lactobacilli on vaginal cultures, than those with RVVC and the women in the COMP group. VVC and a history of RVVC both occurred more frequently in women with a lactobacilli-predominated vaginal flora, as compared with those with a flora change with a mixture of anaerobic and facultative anaerobic bacteria.

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Section: Methodsmentioning

confidence: 99%

Vaginale Flora und venerische Erkrankungen bei Frauen mit rezidivierender oder akuter vulvovaginaler Kandidose

et al. 1995

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“…Cervical samples were collected by swabs provided by the kit producer, (Chlamydiazyme TM , Abbott Laboratories, Chicago, Illinois, or MicroTrak I1 chlamydial EIA (Syva, Palo Alto, California) for enzyme immunoassays (EIA). Positive Chlamydiazyme tests were confirmed by Abbott's Chlamydia confirmatory test 16 .…”

Section: Methodsmentioning

confidence: 99%

Sexual risk behaviour in women carriers of Mycoplasma hominis

Elshibly¹,

Kallings²,

Hellberg³

et al. 1996

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Objective To study the sexual behaviour of women harbouring Mycoplasma hominis in the vagina. Setting Two family planning clinics and a youth clinic for contraceptive advice. Methods The vaginal flora of 996 women were investigated microbiologically, including culturing for M. hominis from the posterior vaginal fornix. Cultures and tests were also made to diagnose genital Chlamydia trachomatis infections, gonorrhoea, genital herpes, cervical human papillomavirus infection, trichomoniasis, genital warts and human immunodeficiency virus. The sexual behaviour of these women was investigated by structured in‐depth personal interviews. Results Of the 996 women, 123 (12.3%) harboured M. hominis in the vagina. The remainder (87.7%), who had a negative M. hominis culture, served as a reference group. Those with M. hominis were significantly more likely to have had more than 10 partners during their lifetime, had a greater frequency of more than one sexual partner during the preceding month and the last six months, and reported more frequent experience of ‘casual sex’, including casual ‘travel sex’. More of them also had experience of group sex and were more likely to have been sexually abused than the women in the reference group. Fewer women with M. hominis had a current steady partner and were less often cohabitors, but more had had sexual intercourse during the previous week than those in the reference group. There was an association between the occurrence of M. hominis and bacterial vaginosis and genital chlamydia1 infection. Adjustment in multifactorial regression analyses therefore changed the results only marginally. Conclusions Women with M. hominis have almost the same sexual risk behaviour as women with classic sexually transmitted diseases.

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“…Voraussetzung fur die Verknupfung der beiden Bedingungen war, den 'cut-off' des ELISA anhand der OD der prainkubierten Proben nach der oben aufgefuhrten Formel zu berechnen (TIJSSEN, 1985). (HIPP et al, 1987;KELLOGG et al, 1990;MARDH et al, 1990;MONCADA et al, 1990;MILLS et al, 1992;OLSEN und SAMBOL, 1993;WARREN et al, 1993) (MILLS et al, 1992).…”

Section: Materials Und Methodenunclassified

“…vorhandenes Chlamydienantigen in der Probe durch Zugabe spezifischer Antikorper zu binden und somit dem Nachweis durch den Fangantikorper des ELISA zu entziehen. Eine im Blocking-Test bewirkte Verringerung der OD um mindestens 50 % wird als Bestatigung der Spezifitat der positiven Reaktion der unbehandelten Probe angesehen(MARDH et al, 1990). Zur Bindung des genusspezifischen Chlamydien-LPS-Antigens in den Proben diente das Serum eines Rindes rnit einer experimentell erzeugten chlamydienbedingten Endometritis(WITTENBRINK et al, 1993~).…”

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Nachweis von Chlamydia psittaci in Vaginalausfluß von Rindern: Eine notwendige Erweiterung der bakteriologischen Diagnostik zur ätiologischen Klärung von Fruchtbarkeitsstörungen beim weiblichen Rind

Wittenbrink

Kirpal

Thiele

et al. 1994

Journal of Veterinary Medicine, Series B

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Vaginal discharge from 119 dairy cows from 59 herds was examined bacteriologically, including application of the IDEIA@ Chlamydia test, to detect genus-specific chlamydial LPSantigen. A putrid quality of specimens was closely correlated with isolation of Actinomyces pyogenes (p < 0.001). The IDEIA was positive for 39 of 65 (60.0 YO) non-putrid specimens and for 16 of 54 (29.6 Yo) putrid specimens; a non-putrid quality of specimens was closely correlated with the detection of chlamydial antigen (p < 0.01). IDEIA-positive results were confirmed by a blocking-antibody assay and by polymerase chain reaction (PCR) using a set of primers based on the 16s rRNA sequences of Chlamydia psittaci. Chlamydia-positive non-putrid specimens generally yielded no other bacterial pathogens. The clinical history of repeat breeding and endornetritis, as well as failure to isolate other bacterial pathogens on routinely used non-living media, were strongly suggestive of primary chlamydial involvement in these cases.

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Use of blocking reagent to confirm enzyme immunoassay results in Chlamydial conjunctivitis

Cited by 14 publications

References 3 publications

Vaginale Flora und venerische Erkrankungen bei Frauen mit rezidivierender oder akuter vulvovaginaler Kandidose

Vaginale Flora und venerische Erkrankungen bei Frauen mit rezidivierender oder akuter vulvovaginaler Kandidose

Sexual risk behaviour in women carriers of Mycoplasma hominis

Nachweis von Chlamydia psittaci in Vaginalausfluß von Rindern: Eine notwendige Erweiterung der bakteriologischen Diagnostik zur ätiologischen Klärung von Fruchtbarkeitsstörungen beim weiblichen Rind

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