Use of an enzyme-linked immunosorbent assay for screening hybridoma antibodies against hepatitis B surface antigen

Boniolo, A.; Dovis, M.; Matteja, R.

doi:10.1016/0022-1759(82)90361-1

Cited by 15 publications

(8 citation statements)

References 12 publications

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“…Hepatitis B surface antigen, hepatitis B core antibody IgM and hepatitis B core antibody IgG were measured using an enzymelinked immunosorbent assay (ELISA) kit (Dia-Sorin, Italy) by standard methods [11][12][13]. Anti-HCV was detected by a 3rd generation ELISA kit (Biochem Immunosystem, Canada) [14], while HCV-RNA extraction was carried out by reverse transcription polymerase chain reaction [15].…”

Section: Serological Analysismentioning

confidence: 99%

Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children

Mahdy¹,

Abd-El-Shaheed²,

Khadr³

et al. 2009

East Mediterr Health J

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Serum levels of glutathione reductase (GR), glutathione S-transferase-α (GST-α) and malondialdehyde (MDA) were determined to evaluate their use in diagnosing hepatocellular damage in 75 children with liver disease. Except for level of GR in patients with HBV, GR, GST-α and MDA were raised significantly in patients compared with controls. At 100% specificity, the sensitivity of the 3 markers for detecting hepatitis B virus, hepatitis C virus and schistosomiasis infection respectively were: 16.7%, 100.0% and 17.7% for GR; 33.3%, 62.1% and 38.2% for GST-α; and 25.0%, 10.3% and 29.4% for MDA. GR was more sensitive in hepatitis C infection, while MDA reflected changes in liver ultrasound and GST-α was the best indicator for histopathological changes. Rôle du statut antioxydant et du processus de péroxydation des lipides dans l'évaluation des lésions hépatocellulaires chez l'enfant RÉSUMÉ Nous avons déterminé les taux sériques de glutathion-réductases (GR), d'α-glutathion-Stransférases (α-GST) et de malondialdéhyde (MDA) afin d'évaluer leur rôle dans le diagnostic des lésions hépatocellulaires chez 75 enfants souffrant d'une maladie hépatique. À l'exception du taux de GR chez les patients atteints du virus de l'hépatite B (VHB), les taux de GR, d'α-GST et de MDA étaient significativement plus élevés chez les malades que chez les témoins. Avec une spécificité de 100 %, la sensibilité des trois marqueurs pour le dépistage du VHB, du VHC et de la schistosomiase était respectivement de 16,7 %, 100,0 % et 17,7 % pour les GR ; de 33,3 %, 62,1 % et 38,2 % pour les α-GST ; et de 25,0 %, 10,3 % et 29,4 % pour le MDA. Les GR étaient plus sensibles dans le cas de l'infection par le VHC, alors que le MDA témoignait de changements à l'échographie du foie et que les α-GST étaient le meilleur indicateur des changements histopathologiques.

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Section: Serological Analysismentioning

confidence: 99%

Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children

Mahdy¹,

Abd-El-Shaheed²,

Khadr³

et al. 2009

East Mediterr Health J

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“…Hepatitis surface antigen (HBsAg), hepatitis B core antibody IgM (HBcAb IgM) and hepatitis B core antibody IgG (HBcAb IgG) were measured by ELISA technique using kits from Dia-Sorin Biomedica Co., according to the methods of Boniolo et al [10] , Tedder and Wilson [11] and Hoofangle et al [12] respectively. Anti-HCV was detected by third generation of ELISA using kit from the Biochem ImmunoSystem Inc [13] , HCV-RNA extraction was carried out by reverse transcription polymerase chain reaction (RT-PCR) according to the method described by Ravaggi et al [14] .…”

Section: Serological Testmentioning

confidence: 99%

Clinical benefits of biochemical markers of bone turnover in Egyptian children with chronic liver diseases

Mahdy

Ahmed²,

Mannaa³

et al. 2007

WJG

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“…This antigen is expressed by many carcinoma lesions including 96% of adenoand adenosquamous lung carcinoma (Thor et al 1986). An indifferent MoAb, 4C4, reactive with hepatitis B surface antigen, was used for comparison in a negative control study (Boniolo et al 1982). Both MoAbs were labeled with iodine 131 by the Iodogen method.…”

Section: Methodsmentioning

confidence: 99%

Endobronchial administration of iodine-131 B72.3 monoclonal antibody in patients with lung cancer

et al. 1991

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We tested the feasibility of endobronchial administration of radiolabelled monoclonal antibodies (MoAbs) and the biodistribution of the radiotracer. Ten patients with histological confirmed adenocarcinoma or squamous cell carcinoma were studied. Nine received 470 gCi (103 gg) of Iodine-131-B72.3, a monoclonal antibody reacting against TAG 72 antigen, while one patient received 502 gCi (291 gg) of 131I-4C4, an indifferent antibody used for comparison in a negative control study. The radiolabelled antibody was administered through a flexible fiberoptic bronchoscope and placed on the tumour mass under visual monitoring. Scans with a large field-of-view gamma-camera showed retention of 131I-B72.3 at the tumour site up to 6-9 days in six of eight patients. No other organs were visualized with the exception of faint activity in the gastrointestinal tract, bladder and thyroid. On the contrary, the indifferent antibody 13~I-4C4 was not retained at the tumour site 6 days after MoAb administration, and more prominent activity was found in the gastrointestinal tract. In one patient the study was not technically adequate because of failure of the delivery system. The vascular compartment contained less than 3% of the administered dose. We conclude that endobronchial administration is a feasible technique and allows stable and specific targetting of bronchial tumours. Furthermore, the low activity found in the plasma and other organs suggests that this approach may be used to deliver therapeutic doses of MoAbs to lung cancers.

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Use of an enzyme-linked immunosorbent assay for screening hybridoma antibodies against hepatitis B surface antigen

Cited by 15 publications

References 12 publications

Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children

Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children

Clinical benefits of biochemical markers of bone turnover in Egyptian children with chronic liver diseases

Endobronchial administration of iodine-131 B72.3 monoclonal antibody in patients with lung cancer

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