Use of a Brine Shrimp Assay To Study Herbal Teas in the Classroom

Opler, Annette; Mizell, Rebecca; Robert, A Bullis; Cervantes‐Cervantes, Miguel; Kincaid, Dwight T.; Kennelly, Edward J.

doi:10.1662/0002-7685(2002)064[0596:uoabsa]2.0.co;2

Cited by 8 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values obtained in this work according to Meyer or Clarkson could be considered with high toxicity. The use of brine shrimp lethality assay to determinate the toxicity of plant extracts has been considered as a preliminary test [33]. This method is simple, reproducible and can be adapted to do in tube or in 96-well microplate.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical screening, antioxidant activity and toxicity of the orchids Prosthechea cochleata and Prosthechea livida-A preliminary study

Avilés

Méndez

Medrano

et al. 2020

JMHE

View full text Add to dashboard Cite

Phytochemical composition, antioxidant, and toxic effects of two orchid (Prosthechea cochleata and Prosthechea livida) were examined in their different hexane and dichloromethane/methanol extracts of leaves, pseudobulbs, rhizome and roots. Phytochemical screening reveals the presence of alkaloids, coumarins, flavonoids, saponins, steroids and terpenes except for anthracene derivatives in P. livida and tannins in both species. Results showed exhibited significant toxicity against brine shrimp with an LC50 values ranged from 3 to 54 µg/ml in 24 h. Finally, the results of the antioxidant test in yeast (Saccharomyces cerevisiae) showed that CH2Cl2:MeOH extract of P. cochleata root extract presented the most efficient protective antioxidant percentage (%PA), with 20 µg/mL, displaying a cell viability of 68.58%. In P. livida case, rhizome extract presented the most efficient %PA, with 1000 µg/mL, with a cell viability of 65.82%. The results evidenced the toxicity of Prosthechea cochleata and Prosthechea livida and reveals potential antioxidative of extracts.

show abstract

Section: Resultsmentioning

confidence: 99%

Phytochemical screening, antioxidant activity and toxicity of the orchids Prosthechea cochleata and Prosthechea livida-A preliminary study

Avilés

Méndez

Medrano

et al. 2020

JMHE

View full text Add to dashboard Cite

show abstract

“…The measurement of toxicity plays a vital role in drug discovery and is a useful tool in biological, especially ecological investigations (Opler et al, 2002). It also serves as a tool for screening plant extracts of possible medicinal value.…”

Section: Brine Shrimp Lethality Bioassaymentioning

confidence: 99%

Screenings of In-vitro antimicrobial, cytotoxic and anti-inflammatory activity of crude methanolic extracts of Crinum latifolium (Leaves)

Rahman¹,

Hussain²,

Millat³

et al. 2016

J. Med. Plants Res.

View full text Add to dashboard Cite

Crude methanolic extracts of Crinum latifolium plant (Leaves) was assayed to identify various pharmacological properties. Antimicrobial potential of crude methanolic extracts of C. latifolium was accomplished by most commonly used disc diffusion method against a wide range of Gram positive (+ve) and Gram negative(-ve) bacteria. Extracts showed slight antimicrobial activity against Gram positive (+ve) bacteria while surprisingly showed significant antimicrobial activity against Gram negative (-ve) bacteria Escherichia coli. In contrast to vincristine sulphate, the crude methanolic, nhexane soluble, petroleum ether soluble and chloroform soluble extracts showed slight to moderate cytotoxic properties with LC 50 value of 7.06, 48.978, 242.83 and 153.93 µg/ml respectively. Plant extract showed significant (P<0.05) anti-inflammatory properties, that is, 16.21 and 20.55%10 mg/ml for hypotonic solution and heat induced condition respectively. So, this plant extract demands further research for revealing all its potency to have new safe drug for the entire respective field of medical science.

show abstract

Brine Shrimp Bioassays: A Useful Technique in Biological Investigations

Rice¹,

Maness²

2004

The American Biology Teacher

View full text Add to dashboard Cite

Use of a Brine Shrimp Assay To Study Herbal Teas in the Classroom

Cited by 8 publications

References 14 publications

Phytochemical screening, antioxidant activity and toxicity of the orchids Prosthechea cochleata and Prosthechea livida-A preliminary study

Phytochemical screening, antioxidant activity and toxicity of the orchids Prosthechea cochleata and Prosthechea livida-A preliminary study

Screenings of In-vitro antimicrobial, cytotoxic and anti-inflammatory activity of crude methanolic extracts of Crinum latifolium (Leaves)

Brine Shrimp Bioassays: A Useful Technique in Biological Investigations

Contact Info

Product

Resources

About