Use of a 96-well microplate reader for measuring routine enzyme activities

Ashour, Mohamed Bassem A; Gee, Shirley J.; Hammock, Bruce D.

doi:10.1016/0003-2697(87)90585-9

Cited by 81 publications

(36 citation statements)

References 15 publications

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“…Plasma glucose (26) and urea (27) concentrations were measured by standard enzymatic colorimetric methods modified for assay using a 96-well plate reader (28). Plasma lactate concentrations were measured by colorimetric assay utilizing the enzyme lactate dehydrogenase and the reduction of NAD.…”

Section: Methodsmentioning

confidence: 99%

Periconceptional Undernutrition Alters Growth Trajectory and Metabolic and Endocrine Responses to Fasting in Late-Gestation Fetal Sheep

2005

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Section: Methodsmentioning

confidence: 99%

Periconceptional Undernutrition Alters Growth Trajectory and Metabolic and Endocrine Responses to Fasting in Late-Gestation Fetal Sheep

2005

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“…All enzyme assays were modified for a microtitre-plate reader and carried out in triplicate (Ashour et al, 1987). NAD + , NADH, NADPH and NAD + were determined enzymically (Bergmeyer & Braβl, 1984).…”

Section: Methodsmentioning

confidence: 99%

Involvement of glutathione in the regulation of respiratory oscillation during a continuous culture of Saccharomyces cerevisiae

Murray

Engelen

Lloyd³

et al. 1999

Microbiology

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Respiratory oscillation occurred during aerobic continuous culture of Saccharomyces cerevisiae. During oscillation, phase-related changes in NAD(P)H and GSH levels occur. Perturbation of oscillation and inhibition of respiration occurred when GSH or GSSG was injected ; however, there was a phase delay in perturbation in the case of an injection during high respiration. The perturbation phase delay was not apparent when a combination of DL-buthionine-(S,R)-sulphoximine, GSH and 5-nitro-2-furaldehyde was injected. Perturbation by GSH injection caused the intracellular GSH concentration to increase, the GSSG concentration to decrease and the cessation of ethanol uptake. NAD(P)H during perturbation was inversely related to dissolved oxygen. Perturbation by calcium pantothenate and pyridoxal-HCl caused a period of enhanced respiration before oscillation returned. These results suggest that the NAD M /NADH redox is not directly involved in oscillation control and regulation involves glutathione metabolism. Possible regulation points include alcohol dehydrogenase inhibition and/or respiratory-chain inhibition.

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“…Plasma glucose concentration was analyzed using standard enzymatic micromethods modified for 96-well microplates (30,31). Plasma insulin concentrations were measured by an RIA established and validated for ovine plasma in this laboratory.…”

Section: Methodsmentioning

confidence: 99%

Birth Weight Rather Than Maternal Nutrition Influences Glucose Tolerance, Blood Pressure, and IGF-I Levels in Sheep

Oliver

2002

Pediatric Research

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Use of a 96-well microplate reader for measuring routine enzyme activities

Cited by 81 publications

References 15 publications

Periconceptional Undernutrition Alters Growth Trajectory and Metabolic and Endocrine Responses to Fasting in Late-Gestation Fetal Sheep

Periconceptional Undernutrition Alters Growth Trajectory and Metabolic and Endocrine Responses to Fasting in Late-Gestation Fetal Sheep

Involvement of glutathione in the regulation of respiratory oscillation during a continuous culture of Saccharomyces cerevisiae

Birth Weight Rather Than Maternal Nutrition Influences Glucose Tolerance, Blood Pressure, and IGF-I Levels in Sheep

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