Urine-derived stem cells: A novel and versatile progenitor source for cell-based therapy and regenerative medicine

Zhang, Deying; Wei, Guanghui; Li, Peng; Zhou, Xiaobo; Zhang, Yuanyuan

doi:10.1016/j.gendis.2014.07.001

Cited by 119 publications

(125 citation statements)

References 68 publications

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“…Prior studies have shown that USCs, similar to iMSCs, have a fast, stable expansion speed and maintain telomere length and high telomerase activity [36,37]. Therefore, USCs may represent a virtually unlimited supply of autologous cells that may be used for cell therapy in rodent models or clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Zhu

Niu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Our previous studies have shown that human urine-derived stem cells (USCs) have great potential as a cell source for cytotherapy and tissue engineering and that extracellular vesicles (EVs) secreted by USCs (USCs-EVs) can prevent diabetes-induced kidney injury in an animal model. The present study was designed to evaluate the effects of USCs-EVs on ischemia repair. Methods: USCs-EVs were isolated and purified by a battery of centrifugation and filtration steps. The USCs-EVs were then characterized by transmission electron microscopy, western blot and tunable resistive pulse sensing techniques. After intramuscularly transplanting USCs-EVs into an ischemic mouse hind-limb, we observed the therapeutic effects of USCs-EVs on perfusion by laser doppler perfusion imaging, angiogenesis and muscle regeneration by histology and immunohistochemistry techniques over 21 days. We subsequently tested whether USCs-EVs can induce the proliferation of a human microvascular endothelial cell line HMEC-1 and a mouse myoblast cell line C2C12 by cell counting kit 8 assay in vitro. Meanwhile, the potential growth factors in the USCs-EVs and supernatants of the USCs cultures were detected by enzyme-linked immunosorbent assay. Results: The USCs-EVs were spherical vesicles with a diameter of 30–150 nm and expressed exosomal markers, such as CD9, CD63 and Tsg101. Ischemic limb perfusion and function were markedly increased in the hind-limb ischemia (HLI) model after USCs-EVs administration. Moreover, angiogenesis and muscle regeneration levels were significantly higher in the USCs-EVs treatment group than in the PBS group. The in vitro experiments showed that USCs-EVs facilitated HMEC-1 and C2C12 cell proliferation in a dose-dependent manner. Conclusions: These results revealed for the first time that USCs-EVs efficiently attenuate severe hind-limb ischemic injury and represent a novel therapy for HLI.

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Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Zhu

Niu

et al. 2018

Cell Physiol Biochem

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“…Examples include fibroblasts isolated from skin biopsies or stem cells isolated from urine (most likely of glomerular parietal epithelial origin) [32–34]. As a result, both patients and healthy controls tend to be willing to donate cells for banking to be used for diagnostic and research purposes.…”

Section: Introductionmentioning

confidence: 99%

Can Human Pluripotent Stem Cell-Derived Cardiomyocytes Advance Understanding of Muscular Dystrophies?

Kalra

Montanaro

Denning

2016

JND

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Muscular dystrophies (MDs) are clinically and molecularly a highly heterogeneous group of single-gene disorders that primarily affect striated muscles. Cardiac disease is present in several MDs where it is an important contributor to morbidity and mortality. Careful monitoring of cardiac issues is necessary but current management of cardiac involvement does not effectively protect from disease progression and cardiac failure. There is a critical need to gain new knowledge on the diverse molecular underpinnings of cardiac disease in MDs in order to guide cardiac treatment development and assist in reaching a clearer consensus on cardiac disease management in the clinic. Animal models are available for the majority of MDs and have been invaluable tools in probing disease mechanisms and in pre-clinical screens. However, there are recognized genetic, physiological, and structural differences between human and animal hearts that impact disease progression, manifestation, and response to pharmacological interventions. Therefore, there is a need to develop parallel human systems to model cardiac disease in MDs. This review discusses the current status of cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSC) to model cardiac disease, with a focus on Duchenne muscular dystrophy (DMD) and myotonic dystrophy (DM1). We seek to provide a balanced view of opportunities and limitations offered by this system in elucidating disease mechanisms pertinent to human cardiac physiology and as a platform for treatment development or refinement.

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“…It has been widely reported that stem cells can secrete paracrine factors to help the resident cells to survival and perform normal function as well as regulate the local micro‐environment (Jeon et al, ; Liang, Ding, Zhang, Tse, & Lian, ). Recently, it has been reported that urine‐derived stem cells (USCs) is also a stem cell candidate for tissue regeneration as it has the characters of stem cells (Bodin et al, ; Zhang, Wei, Li, Zhou, & Zhang, ). It was reported that USCs were positive for CD29, CD44, CD73, and CD90 antigen, and negative for CD31, CD34, CD45, CD133, CD309, and HLA‐DR.…”

Section: Introductionmentioning

confidence: 99%

Bioglass enhanced wound healing ability of urine‐derived stem cells through promoting paracrine effects between stem cells and recipient cells

Zhang

Niu

Dong

et al. 2017

J Tissue Eng Regen Med

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In cell therapy, tissue regeneration ability of stem cells relies on the paracrine effects between stem cells and recipient cells. Our recent studies demonstrated that, in tissue engineering, bioactive silicates could stimulate paracrine effects between stem cells and recipient cells, which enhanced tissue generation. Therefore, we proposed that, in cell therapy, it may be an effective method to improve tissue regeneration ability of stem cells through activating the paracrine effects between stem cells and recipient cells with bioactive silicates. As urine-derived stem cells (USCs) have been injected for wound healing and bioglass (BG) have shown bioactivity for various types of stem cells, in this study, we activated USCs with effective BG ionic products. Then the conditioned medium of BG-activated USCs were used to culture endothelial cells and fibroblasts as well as co-cultures of endothelial cells and fibroblasts. Results showed that growth factor expression in BG-activated USCs was upregulated. In addition, paracrine effects between USCs and recipient cells in wound healing were stimulated, which resulted in enhanced capillary-like network formation of endothelial cells and matrix protein production as well as myofibroblast differentiation of fibroblasts. Finally, the BG-activated USCs were applied on full-thickness excisional wounds. Results confirmed that BG-activated USCs had better wound healing ability through improving angiogenesis and collagen deposition in wound sites as compared with USCs without any treatment. Taken together, BG can be used to promote wound healing ability of USCs by enhancing paracrine effects between USCs and recipient cells.

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Urine-derived stem cells: A novel and versatile progenitor source for cell-based therapy and regenerative medicine

Cited by 119 publications

References 68 publications

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Can Human Pluripotent Stem Cell-Derived Cardiomyocytes Advance Understanding of Muscular Dystrophies?

Bioglass enhanced wound healing ability of urine‐derived stem cells through promoting paracrine effects between stem cells and recipient cells

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