Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease - A Pilot Study

Korenkova, Vlasta; Jones, Alun; Hoy, Wendy E.; Morais, Christudas; Cooper, Matthew A.; Gobé, Glenda C.

doi:10.4172/2161-0444.1000249

Cited by 2 publications

(3 citation statements)

References 36 publications

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“… 58 AMBP has been proposed as a biomarker of tubular dysfunction in the early stages of diabetic nephropathy and in the late stages of chronic kidney disease. 59 – 62 Increased excretion urinary of AMBP has been frequently identified in patients with diabetes, compared with the control group. 57 Increased levels of AMBP suggest that serum AMBP may play a role in the regulation of inflammatory processes, 62 such as that occurs in diabetes.…”

Section: Discussionmentioning

confidence: 99%

“… 59 – 62 Increased excretion urinary of AMBP has been frequently identified in patients with diabetes, compared with the control group. 57 Increased levels of AMBP suggest that serum AMBP may play a role in the regulation of inflammatory processes, 62 such as that occurs in diabetes. Interestingly, our study also showed that increased levels of AMBP occur in the serum proteome of DM1 patients, and to the best of our knowledge, this is the first study to observe this protein in serum from DM1 patients.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis to identify candidate biomarkers associated with type 1 diabetes

Oliveira

Lima-Neto

Tilburg

et al. 2018

DMSO

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PurposeType 1 diabetes mellitus (DM1) is one of the most common chronic diseases observed during childhood. The incidence of DM1 is increasing worldwide, and there is currently no way to prevent or delay the onset or to cure the disease. Most diseases, including diabetes, stem from abnormalities in the functioning of proteins, and some studies have reported the expression of protein variation to be involved in the development of DM1. Thus, the aim of this study was to investigate the differential expression of serum proteins in patients with DM1.Materials and methodsSerum of patients with DM1 (n=30) and healthy controls (n=30) was collected. A proteomic approach was used with depletion of albumin and immunoglobulin G chromatography on serum samples followed by data-independent, label-free mass spectrometric analysis.ResultsA total of eight serum proteins were identified as being differentially expressed and involved in the immune system, lipid metabolism, and pathways of coagulation. DM1 was associated with the upregulation of six proteins: alpha-2-macroglobulin, apolipoprotein A-II, β2 glycoprotein I, Ig alpha-2 chain C region, alpha-1-microglobulin, and prothrombin. A total of two proteins were downregulated, including pregnancy zone protein and complement C4.ConclusionTo the best of our knowledge, these findings show differential expression of proteins revealing new proteins that may be involved in the development and progression of diabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic analysis to identify candidate biomarkers associated with type 1 diabetes

Oliveira

Lima-Neto

Tilburg

et al. 2018

DMSO

View full text Add to dashboard Cite

show abstract

“…All HE and PAS-stained tissue sections were viewed using light microscopy and assessed for morphological characteristics, including hydropic change, apoptosis and necrosis, in 10 microscope fields per section at ×200 magnification by the protocol of Wunnapuk et al [ 37 ]. Hematological parameters were measured for each sample ( n = 1) in the Chemical Pathology Department at the Princess Alexandra Hospital (Brisbane, Australia) by the Jaffe method using a Beckman DxC800 general chemistry analyser (Beckman Coulter, Brea, CA, USA), following the protocol of Korenkova et al [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Anti-Coagulant and Anti-Thrombotic Properties of Blacklip Abalone (Haliotis rubra): In Vitro and Animal Studies

et al. 2017

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Sulphated polysaccharides with anti-thrombotic and anti-coagulant activities have been found in various marine biota. In this study, a previously characterised anti-thrombotic and anti-coagulant extract from blacklip abalone was fractionated by anion exchange chromatography (AEC), pooled (on a sulphated polysaccharide basis) and administered to Wistar rats via oral gavage (N = 8) for assessment as an oral therapeutic. To ensure that the preparation had anti-coagulant activity prior to oral administration, it was assessed in rat blood by thromboelastography (TEG) significantly increasing reaction (R) time (or time until clot formation). Following in vitro confirmation of anti-coagulant activity, 40 mg of the preparation was orally administered to rats with blood samples collected at 2, 4, and 6 h post-gavage. Assessment of all blood samples by TEG showed some prolongation of R time from 355 to 380 s after 4 h. Dosing of the post-gavage blood samples with the abalone preparation to confirm anti-thrombotic activity in vitro revealed residual anti-coagulant activity, further suggesting that oral administration did increase anti-coagulant potential in the collected blood but that bioavailability was low. Assessment of tissues and haematological parameters showed no obvious harmful effects of the abalone preparation in animals. In summary, even though oral administration of fractionated and pooled blacklip abalone extract to rats delayed clotting after 4 h, bioavailability of the preparation appeared to be low and may be more appropriate for intravenous administration as an anti-thrombotic or anti-coagulant therapeutic.

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Urinary Biomarkers for Detection of Early and Advanced Chronic Kidney Disease - A Pilot Study

Cited by 2 publications

References 36 publications

Proteomic analysis to identify candidate biomarkers associated with type 1 diabetes

Proteomic analysis to identify candidate biomarkers associated with type 1 diabetes

Anti-Coagulant and Anti-Thrombotic Properties of Blacklip Abalone (Haliotis rubra): In Vitro and Animal Studies

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