Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer

Różalski, Rafał; Gackowski, Daniel; Siomek-Górecka, Agnieszka; Starczak, Marta; Modrzejewska, Martyna; Banaszkiewicz, Zbigniew; Oliński, Ryszard

doi:10.3109/1354750x.2015.1068860

Cited by 38 publications

(26 citation statements)

References 25 publications

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“…Rozalski and co-workers investigated 8-oxo-7,8-dihydro-2'-deoxyguanosine, 8-oxo-7,8-dihydroguanine, and 5-hydroxymethyluracil. Group data and the main findings are reported in table 1, whereas SP and SE values are listed in table 3 71.…”

Section: Resultsmentioning

confidence: 99%

“…Since oxidative stress related to chronic inflammation is associated with CRC etiology, Rozalski and colleagues assessed urinary excretion of oxidatively modified DNA bases/nucleoside (8-oxo-7,8-dihydroguanine, 8-oxo-7,8-dihydro-2'-deoxyguanosine and 5-hydroxymethyluracil) in HC, adenoma patients and CRC patients 71. Although urinary DNA changes may reflect the oxidative stress/chronic inflammation found in CRC 85,86, its performance in early diagnosis is moderate 71.…”

Section: Discussionmentioning

confidence: 99%

“…Although urinary DNA changes may reflect the oxidative stress/chronic inflammation found in CRC 85,86, its performance in early diagnosis is moderate 71.…”

Section: Discussionmentioning

confidence: 99%

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Role of Urinary Biomarkers in the Diagnosis of Adenoma and Colorectal Cancer: A Systematic Review and Meta-Analysis

Altobelli¹,

Angeletti²,

Latella³

2016

J. Cancer

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The growing interest in enhancing and spreading colorectal cancer (CRC) screening has been stimulating the exploration of novel biomarkers with greater sensitivity and specificity than immunochemical faecal occult blood test (iFOBT). The present study provides i) a systematic review of the urinary biomarkers that have been tested to achieve early CRC diagnosis and assess the risk of colorectal adenoma and adenocarcinoma, and ii) a meta-analysis of the data regarding the urinary prostaglandin (PG) metabolite PGE-M.As regard to gene markers, we found significantly different percent methylation of the vimentin gene in CRC patients and healthy controls (HC) (p<0.0001). Respect to metabolism of nitrogenous bases, cytidine, 1-methyladenosine, and adenosine, have higher concentrations in CRC patients than in HC (respectively, p<0.01, p=0.01, and p<0.01). As regard to spermine we found that N1,N12 diacetyl spermine (DiAcSpm) and N1, N8 diacetylspermidine (DiAcSpd) were significantly higher in CRC than in HC (respectively p=0.01 and p<0.01). Respect to PGE-M, levels were higher in CRC than in those with multiple polyposis (p<0.006) and HC subjects (p<0.0004). PGE-M seems to be the most interesting and promising urinary marker for CRC and adenoma risk assessment and for CRC screening.In conclusion, evidence suggests that urinary biomarker could have a potential role as urinary biomarkers in the diagnosis of colorectal cancer. Particularly, PGE-M seems to be the most promising urinary marker for CRC early detection.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Role of Urinary Biomarkers in the Diagnosis of Adenoma and Colorectal Cancer: A Systematic Review and Meta-Analysis

Altobelli¹,

Angeletti²,

Latella³

2016

J. Cancer

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“…A number of analytical techniques for measurement of urinary 8-OHdG have been developed, including enzyme-linked immunosorbent assay (ELISA)333435, capillary electrophoresis with various detectors (e.g., electrochemical or ultraviolet detection, CE-ECD or CE-UV)363738, high-performance liquid chromatography with electrochemical detection (HPLC-ECD)3940, gas chromatography-mass spectrometry (GC-MS)414243, and liquid chromatography-tandem mass spectrometry (LC-MS/MS)33344445464748495051. ELISA is the most convenient method and has received widespread use, but lack of specificity seems to be the main problem and several reports have demonstrated that ELISA frequently overestimates urinary 8-OHdG levels compared with the concentrations measured by chromatography-based techniques.…”

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confidence: 99%

Association between Oxidative DNA Damage and Risk of Colorectal Cancer: Sensitive Determination of Urinary 8-Hydroxy-2′-deoxyguanosine by UPLC-MS/MS Analysis

Guo

Wang

et al. 2016

Sci Rep

120

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Oxidative DNA damage plays crucial roles in the pathogenesis of numerous diseases including cancer. 8-hydroxy-2′-deoxyguanosine (8-OHdG) is the most representative product of oxidative modifications of DNA, and urinary 8-OHdG is potentially the best non-invasive biomarker of oxidative damage to DNA. Herein, we developed a sensitive, specific and accurate method for quantification of 8-OHdG in human urine. The urine samples were pretreated using off-line solid-phase extraction (SPE), followed by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. By the use of acetic acid as an additive to the mobile phase, we improved the UPLC-MS/MS detection of 8-OHdG by 2.7−5.3 times. Using the developed strategy, we measured the contents of 8-OHdG in urine samples from 142 healthy volunteers and 84 patients with colorectal cancer (CRC). We observed increased levels of urinary 8-OHdG in patients with CRC and patients with tumor metastasis, compared to healthy controls and patients without tumor metastasis, respectively. Additionally, logistic regression analysis and receiver operator characteristic (ROC) curve analysis were performed. Our findings implicate that oxidative stress plays important roles in the development of CRC and the marked increase of urinary 8-OHdG may serve as a potential liquid biomarker for the risk estimation, early warning and detection of CRC.

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“…It has been postulated that 8-oxodG is one of the sensitive biomarkers for oxidative damage of cellular DNA [13]. 8-oxodG is a useful marker for evaluating the cardiovascular risk factors and inflammatory status in hypertensive patients [14], for identifying persons at risk of developing cancer [15], for early prediction of lifestyle-related disease risk [16], and for assessing the environmental conditions that are expected to have a serious long-term impact on the health status in adults [17].…”

Section: Introductionmentioning

confidence: 99%

A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in human urine and plasma

Bartošová

Riman

Halouzka

et al. 2016

Analytica Chimica Acta

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Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer

Abstract: Urinary DNA modifications may reflect the oxidative stress/chronic inflammation in colorectal cancer but diagnostic performance for early-detection is moderate.

Cited by 38 publications

References 25 publications

Role of Urinary Biomarkers in the Diagnosis of Adenoma and Colorectal Cancer: A Systematic Review and Meta-Analysis

Role of Urinary Biomarkers in the Diagnosis of Adenoma and Colorectal Cancer: A Systematic Review and Meta-Analysis

Association between Oxidative DNA Damage and Risk of Colorectal Cancer: Sensitive Determination of Urinary 8-Hydroxy-2′-deoxyguanosine by UPLC-MS/MS Analysis

A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in human urine and plasma

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