Uridine uptake inhibition as a cytotoxicity test: Correlations with the Draize test

Final Report on the Safety Assessment of Methyl Alcohol

“…For the BHK ¡21/C13 cells, the concentration of Methyl Alcohol that induced a toxic effect during a cell detachment assay was 1230 mM, which corresponded with a low cytotoxicity ranking (Reinhardt, Pelli, and Zbinden 1985). In studies using mouse Balb/c 3T3 broblasts, the highest tolerated dose was 864 mM Methyl Alcohol (Borenfreund and Borrero 1984) and the concentration that induced 50% inhibition in [ 3 H]uridine uptake was 668 mM Methyl Alcohol (Shopsis and Sathe 1984). The highest tolerated dose was 1037.4 mM in Chinese Hamster V79 broblasts, 617.5 mM in the rabbit cornea epithelium, 111.5 mM in human hepatoma (HepG 2 ) cells, and 1037.4 mM in murine macrophages (RAW 264.7) (Borenfreund and Borrero 1984;Borenfreund and Shopsis 1985).…”

Final Report on the Safety Assessment of Methyl Alcohol

“…For the BHK ¡21/C13 cells, the concentration of Methyl Alcohol that induced a toxic effect during a cell detachment assay was 1230 mM, which corresponded with a low cytotoxicity ranking (Reinhardt, Pelli, and Zbinden 1985). In studies using mouse Balb/c 3T3 broblasts, the highest tolerated dose was 864 mM Methyl Alcohol (Borenfreund and Borrero 1984) and the concentration that induced 50% inhibition in [ 3 H]uridine uptake was 668 mM Methyl Alcohol (Shopsis and Sathe 1984). The highest tolerated dose was 1037.4 mM in Chinese Hamster V79 broblasts, 617.5 mM in the rabbit cornea epithelium, 111.5 mM in human hepatoma (HepG 2 ) cells, and 1037.4 mM in murine macrophages (RAW 264.7) (Borenfreund and Borrero 1984;Borenfreund and Shopsis 1985).…”

Basic Elements of Toxicology

“…In order to reduce the use of animals for eye irritancy testing, a variety of alternative procedures have been proposed, which include the use of enucleated eyes, various in vitro cell or tissue cultures, and the noninvasive measurement of corneal thickness (Nardone and Bradlaw, 1983;Shopsis and Sathe, 1984;Ballantyne, 1986;Borenfreund and Borrero, 1984 …”

Environmental Chemicals

“…Combinations of agents with differing functional groups produced additive effects on the inhibition of uridine uptake, suggesting the utility of this approach for the analysis of mixtures ( Shopsis and Sathe, 1984).…”

Final Report on the Safety Assessment of Methyl Alcohol

“…For the BHK ¡21/C13 cells, the concentration of Methyl Alcohol that induced a toxic effect during a cell detachment assay was 1230 mM, which corresponded with a low cytotoxicity ranking (Reinhardt, Pelli, and Zbinden 1985). In studies using mouse Balb/c 3T3 broblasts, the highest tolerated dose was 864 mM Methyl Alcohol (Borenfreund and Borrero 1984) and the concentration that induced 50% inhibition in [ 3 H]uridine uptake was 668 mM Methyl Alcohol (Shopsis and Sathe 1984). The highest tolerated dose was 1037.4 mM in Chinese Hamster V79 broblasts, 617.5 mM in the rabbit cornea epithelium, 111.5 mM in human hepatoma (HepG 2 ) cells, and 1037.4 mM in murine macrophages (RAW 264.7) (Borenfreund and Borrero 1984;Borenfreund and Shopsis 1985).…”

Basic Elements of Toxicology

“…In order to reduce the use of animals for eye irritancy testing, a variety of alternative procedures have been proposed, which include the use of enucleated eyes, various in vitro cell or tissue cultures, and the noninvasive measurement of corneal thickness (Nardone and Bradlaw, 1983;Shopsis and Sathe, 1984;Ballantyne, 1986;Borenfreund and Borrero, 1984 …”

Environmental Chemicals

“…Combinations of agents with differing functional groups produced additive effects on the inhibition of uridine uptake, suggesting the utility of this approach for the analysis of mixtures ( Shopsis and Sathe, 1984).…”

Final Report on the Safety Assessment of Methyl Alcohol

“…For the BHK ¡21/C13 cells, the concentration of Methyl Alcohol that induced a toxic effect during a cell detachment assay was 1230 mM, which corresponded with a low cytotoxicity ranking (Reinhardt, Pelli, and Zbinden 1985). In studies using mouse Balb/c 3T3 broblasts, the highest tolerated dose was 864 mM Methyl Alcohol (Borenfreund and Borrero 1984) and the concentration that induced 50% inhibition in [ 3 H]uridine uptake was 668 mM Methyl Alcohol (Shopsis and Sathe 1984). The highest tolerated dose was 1037.4 mM in Chinese Hamster V79 broblasts, 617.5 mM in the rabbit cornea epithelium, 111.5 mM in human hepatoma (HepG 2 ) cells, and 1037.4 mM in murine macrophages (RAW 264.7) (Borenfreund and Borrero 1984;Borenfreund and Shopsis 1985).…”

Basic Elements of Toxicology

“…In order to reduce the use of animals for eye irritancy testing, a variety of alternative procedures have been proposed, which include the use of enucleated eyes, various in vitro cell or tissue cultures, and the noninvasive measurement of corneal thickness (Nardone and Bradlaw, 1983;Shopsis and Sathe, 1984;Ballantyne, 1986;Borenfreund and Borrero, 1984 …”

Environmental Chemicals

“…Combinations of agents with differing functional groups produced additive effects on the inhibition of uridine uptake, suggesting the utility of this approach for the analysis of mixtures ( Shopsis and Sathe, 1984).…”