Urease covalently immobilized on cotton-derived nanocellulose-dialdehyde for urea detection and urea-based multicomponent synthesis of tetrahydro-pyrazolopyridines in water

Tamaddon, Fatemeh; Arab, Davood

doi:10.1039/c9ra05240b

Cited by 24 publications

(8 citation statements)

References 49 publications

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“…These miniaturized nanostructures are preferred to enhance the sensing ability of urea because they offer a higher surface area leading to a higher adsorption of urea analyte onto the nanofibrous surface as well as a better porosity leading to an improved diffusion within the nanofibrous sensor bulk. Therefore, the prepared CNF-TCFH-U biosensor exhibited an improved sensitivity upon exposure to NH 3 liberated from reacting urease enzyme with CO(NH 2 ) 2 [ 51 ]. The morphologies of CNF-TCFH-U nanofibers showed increasing in the density of calcium alginate nanoparticles with increasing their concentration.…”

Section: Resultsmentioning

confidence: 99%

Solution Blowing Spinning Technology towards Green Development of Urea Sensor Nanofibers Immobilized with Hydrazone Probe

2021

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Cellulose has been one of the most widespread materials due to its renewability, excellent mechanical properties, biodegradability, high absorption ability, biocompatibility and cheapness. Novel, simple and green colorimetric fibrous film sensor was developed by immobilization of urease enzyme (U) and tricyanofuran hydrazone (TCFH) molecular probe onto cellulose nanofibers (CNF). Cellulose acetate nanofibers (CANF) were firstly prepared from cellulose acetate using the simple, green and low cost solution blowing spinning technology. The produced CANF was exposed to deacetylation to introduce CNF, which was then treated with a mixture of TCFH and urease enzyme to introduce CNF-TCFH-U nanofibrous biosensor. CNF were reinforced with tricyanofuran hyrazone molecular probe and urease enzyme was encapsulated into calcium alginate biopolymer to establish a biocomposite film. This CNF-TCFH-U naked-eye sensor can be applied as a disposable urea detector. CNF demonstrated a large surface area and was utilized as a carrier for TCFH, which is the spectroscopic probe and urease is a catalyst. The biochromic CNF-TCFH-U nanofibrous biosensor responds to an aqueous medium of urea via a visible color signal changing from off-white to dark pink. The morphology of the generated CNF and CNF-TCFH-U nanofibrous films were characterized by different analytical tools, including energy-dispersive X-ray patterns (EDX), polarizing optical microscope (POM), Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). SEM images of CNF-TCFH-U nanofibers demonstrated diameters between 800 nm and 2.5 μm forming a nonwoven fabric with a homogeneous distribution of TCFH/urease-containing calcium alginate nanoparticles on the surface of CNF. The morphology of the cross-linked calcium alginate nanoparticles was also explored using transmission electron microscopy (TEM) to indicate an average diameter of 56–66 nm. The photophysical performance of the prepared CNF-TCFH-U was also studied by CIE Lab coloration parameters. The nanofibrous film biosensor displayed a relatively rapid response time (5–10 min) and a limit of detection as low as 200 ppm and as high as 1400 ppm. Tricyanofuran hydrazone is a pH-responsive disperse dye comprising a hydrazone detection group. Determination of urea occurs through a proton transfer from the hydrazone group to the generated ammonia from the reaction of urea with urease.

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Section: Resultsmentioning

confidence: 99%

Solution Blowing Spinning Technology towards Green Development of Urea Sensor Nanofibers Immobilized with Hydrazone Probe

2021

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“…After the development of aldehyde group on nanocellulose the urease enzyme was covalently immobilised by amino acid and CHO group. And green urease biosensor had the application to detect the urea in blood serum and aqueous sample [ 92 ]. Surface‐enhanced Raman Scattering paired with silver dendrites was used first time to detect limited urea concentration.…”

Section: Classification Of Urea Biosensorsmentioning

confidence: 99%

Recent advancements in urea biosensors for biomedical applications

Singh

Sharma

Singh

2021

IET Nanobiotechnology

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The quick progress in health care technology as a recurrent measurement of biochemical factors such as blood components leads to advance development and growth in biosensor technology necessary for effectual patient concern. The review wok of authors present a concise information and brief discussion on the development made in the progress of potentiometric, field effect transistor, graphene, electrochemical, optical, polymeric, nanoparticles and nanocomposites based urea biosensors in the past two decades. The work of authors is also centred on different procedures/methods for detection of urea by using amperometric, potentiometric, conductometric and optical processes, where graphene, polymer etc. are utilised as an immobilised material for the fabrication of biosensors. Further, a comparative revision has been accomplished on various procedures of urea analysis using different materials-based biosensors, and it discloses that electrochemical and potentiometric biosensor is the most promise one among all, in terms of rapid response time, extensive shelf life and resourceful design.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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“…Tamaddon and Arab 80 mentioned the HANCD@Urs contained an environment‐friendly green protocol for urea detection. The HANCD was prepared by nanocellulose‐dialdehyde (HANCD), which was obtained from cotton‐derived nanocellulose (NC) via tandem acid‐hydrolysis and periodate‐oxidation reactions.…”

Section: Development Of Urea Biosensor Based On Immobilization Matrixmentioning

confidence: 99%

Urea biosensors: A comprehensive review

Botewad

Gaikwad²,

Girhe

et al. 2021

Biotech and App Biochem

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Present study is specially designed for the recent advances in biosensors to detect and quantify urea concentration. Urea (carbamide) is an organic compound made up of the carbonyl (C=O) functional group with two −NH2 groups having chemical formula CO (NH2)2. In nature, urea is found everywhere as the result of various processes, and in the human body, urea is an end product of nitrogen metabolism. An excessive concentration of urea in the human body is responsible for different critical diseases such as indigestion, acidity, ulcers, cancer, malfunctioning of kidneys, renal failure, urinary tract obstruction, dehydration, shock, burns, gastrointestinal bleeding, and so on. Moreover, below the normal level may cause hepatic failure, nephritic syndrome, cachexia, and so on. As well as in various fields such as fishery, dairy, food preservation, agriculture, and so on, urea is normally found and its detection is necessary. In urea biosensors, enzyme urease (Urs) is used as a bioreceptor element and retains its long last activity is the critical issue in front of the researcher. During recent decades, different nanoparticles (zinc oxide, nickel oxide, iron oxide, titanium dioxide, tin(IV) oxide, etc.), conducting polymer (polyaniline, polypyrrole, etc.), conducting polymer–nanoparticles composites, carbon materials (carbon nanotubes, graphene oxide, reduced graphene oxide graphene), and so on are used in urea biosensors. The main emphasis of the present study is to provide cumulative and comprehensive information about the sensing parameters of urea biosensors based on the materials used for enzyme immobilization. Besides this special task, this review provides a fruitful discussion on the basics of biosensors briefly for new and upcoming researchers. Thus, the present study may act as a gift for a large audience that come from different fields and are working in biosensors research.

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Urease covalently immobilized on cotton-derived nanocellulose-dialdehyde for urea detection and urea-based multicomponent synthesis of tetrahydro-pyrazolopyridines in water

Abstract: The urease Schiff-base covalently bonded to the designed high-content nanocellulosedialdehyde (HANCD) prepared from cotton-derived nanocellulose (NC) via tandem acid-hydrolysis and periodate-oxidation reactions was termed HANCD@urease.

Cited by 24 publications

References 49 publications

Solution Blowing Spinning Technology towards Green Development of Urea Sensor Nanofibers Immobilized with Hydrazone Probe

Solution Blowing Spinning Technology towards Green Development of Urea Sensor Nanofibers Immobilized with Hydrazone Probe

Recent advancements in urea biosensors for biomedical applications

Urea biosensors: A comprehensive review

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