Urease activity in soybean meal products

Smith, A. K.; Belter, P. A.; Anderson, Robert L.

doi:10.1007/bf02665111

Cited by 10 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The urease activity was determined by the titration method. 46 Briefly, 1.8 mg of urease was added to the buffer solution (3.6 mL, pH either 4 or 7) containing urea (30 mg/mL) in a test tube at 25 °C, with or without ADH (14 mg/mL) and/or PAM-co-PDAAM polymer (135 mg/mL). After 30 min, the enzymatic reaction was terminated by adding 30 mL of HCl (0.12 M).…”

Section: Synthesis Of Polymersmentioning

confidence: 99%

Bioinspired Self-Healing of Kinetically Inert Hydrogels Mediated by Chemical Nutrient Supply

Zhong

Hao

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Dynamic stability and self-healing ability are two inherently compatible properties for living organisms. By contrast, kinetic stability and intrinsic healability are two desired but mostly incompatible properties for synthetic materials. This is because the healing of these materials heavily relies on the kinetic lability of the chemical bonds or physical interactions in materials. Inspired by the hierarchically and temporally controlled wound healing in biological systems, here, we report the intrinsic healing of kinetically stable hydrogels, regulated by the consumption of chemical nutrients. The acylhydrazone-based polymer hydrogels with preinstalled urease and urea were formed at a low initial pH, followed by in situ enzymatic generation of a base to deactivate the dynamic bonds, allowing efficient fabrication of kinetically stable hydrogels. The healing of damaged hydrogels was effective when fed with proper chemical nutrients (i.e., acidic urea solutions), in which case a transient acidic pH state was temporally programmed by combining a fast acidic activator (for structural healing) with the slow, biocatalytic generation of a base (for property recovery). The ability to regulate both hydrogel fabrication and healing via a single enzymatic reaction could provide a new approach to create kinetically stable materials capable of healing damages on demand.

show abstract

Section: Synthesis Of Polymersmentioning

confidence: 99%

Bioinspired Self-Healing of Kinetically Inert Hydrogels Mediated by Chemical Nutrient Supply

Zhong

Hao

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…One study [113] proposed an alternative method, with the potential to differentiate between meals with low levels of urease activity, based on the incubation of the meal with urea in a buffered solution and the colorimetric determination of the residual urea with pdimethylaminobenzaldehyde. A method for direct titration of ammonia as a measure of urease activity was proposed [114] and adapted [115], in which the incubation of urea and the meal is performed, maintaining the pH of the solution by slowly adding HCl. The system is then titrated with NaOH.…”

Section: Soybean As Animal Feed -What Urease Has Got To Do With It?mentioning

confidence: 99%

Soybean Urease: Over a Hundred Years of Knowledge

Real‐Guerra¹,

Stanisçuaski²,

Carlini³

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

View full text Add to dashboard Cite

“…These values were on lower end of range of values reported for SBM samples from different geographical regions (55 samples, 70%-77%, Ravindran et al 2014;515 samples, 68%-96%, García-Rebollar et al 2016). The urease index indicates if applied heating is enough to destroy trypsin inhibitors (Smith et al 1956). The observed urease index was within the range of 0.00-0.25 reported for SBM from US, Brazil, India, and Argentina (Ravindran et al 2014;García-Rebollar et al 2016).…”

Section: Resultsmentioning

confidence: 99%

Standardized ileal digestibility of amino acids and apparent metabolizable energy in corn and soybean meal for organic broiler chicken production in Ontario

Leung

Kiarie

2020

Can. J. Anim. Sci.

View full text Add to dashboard Cite

Standardized (SID) digestibility of amino acids (AA) and apparent metabolizable energy (AMEn) in samples of organic corn and soybean meal (SBM) were determined. Conventional corn (CC) and SBM (CSBM) samples were tested for comparison. A total of 560, 14 d old male broiler chickens (Cobb 500) were weighed, placed in cages (10 birds/cage) and allocated to 7 (n=8) semi-purified wheat starch-based diets. Diets were: 1) CC, 2) imported organic corn (OCI), 3) local organic corn (OCL), 4) CSBM, 5) imported organic SBM (OSBMI), 6) local organic SBM (OSBML) and 7) nitrogen-free wheat starch (NFD). Only a few differences were observed on SID of AA; SID of lysine was lower (P=0.002) in organic corn samples relative to CC and SID of methionine was lower (P=0.002) in OSBML sample relative to CSBM and OSBMI samples. The AMEn of CC was higher (P<0.01) than that of organic corn samples. The AMEn of OSBML was higher (P<0.001) than for CSBM and OSBMI, however, the value for OSBMI was higher (P<0.001) than for CSBM. In conclusions, the utilization of amino acids in conventional and organic feedstuffs were comparable, however, differences in energy utilization warrant considerations in organic broiler feed formulation.

show abstract

Urease activity in soybean meal products

Cited by 10 publications

References 8 publications

Bioinspired Self-Healing of Kinetically Inert Hydrogels Mediated by Chemical Nutrient Supply

Bioinspired Self-Healing of Kinetically Inert Hydrogels Mediated by Chemical Nutrient Supply

Soybean Urease: Over a Hundred Years of Knowledge

Standardized ileal digestibility of amino acids and apparent metabolizable energy in corn and soybean meal for organic broiler chicken production in Ontario

Contact Info

Product

Resources

About