Uptake of secondary autoxidation products of linoleic acid by the rat

Kanazawa, Kazuki; Kanazawa, Eri; Natake, Masato

doi:10.1007/bf02534231

Cited by 105 publications

(49 citation statements)

References 37 publications

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“…The current postprandial changes in HDL lipid composition including increased triglyceride and phospholipid content and decreased cholesteryl ester content after the meals were generally in line with the corresponding postprandial changes reported previously. 49 Animal studies suggest that secondary lipid oxidation products, including low molecular weight carbonyl compounds, are absorbed from the gut, 20,24,25 inhibit enzyme activities, 25 and form conjugates with glutathione, 24 a thiol containing compound. Derivatisation of cysteine thiol groups on paraoxonase inhibits its activity.…”

Section: Discussionmentioning

confidence: 99%

“…Detoxification by a glutathione peroxidase cycle in the gut 23 may severely limit absorption of dietary lipid hydroperoxides. However, secondary lipid oxidation products are more readily absorbed 20,24 and can inhibit hepatic enzymes in animals. 25 In mice that are susceptible to arterial lesion development, an atherogenic diet and injection of mildly oxidized LDL and oxidized lipids into the circulation reduces serum paraoxonase activity.…”

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confidence: 99%

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Reduced Postprandial Serum Paraoxonase Activity After a Meal Rich in Used Cooking Fat

Sutherland

Walker

Jong

et al. 1999

ATVB

120

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Abstract-Paraoxonase is an enzyme associated with HDL in human serum that hydrolyzes oxidized phospholipids and inhibits LDL oxidation, which is an important step in atherogenesis. In animals, addition of oxidized lipids to the circulation reduces paraoxonase activity, and diets rich in oxidized fat accelerate the development of atherosclerosis. The current randomized, crossover study was designed to compare the effect of a meal rich in oxidized lipids in the form of fat that had been used for deep-frying in a fast food restaurant and a control meal rich in the corresponding unused fat on postprandial serum paraoxonase (arylesterase) activity and peroxide content of LDL and its susceptibility to copper ion catalyzed oxidation in 12 healthy men. Four hours into the postprandial period, serum paraoxonase activity had decreased significantly after the used fat meal (Ϫ17%, Pϭ0.005) and had increased significantly after the meal rich in unused fat (14%, Pϭ0.005). These changes were significantly (Pϭ0.003) different. A time-course study indicated that serum paraoxonase activity remained lower than baseline for up to 8 hours after the used fat meal. Serum apoA1 concentration tended to decrease after the unused fat meal and tended to increase after the used fat meal. These changes were different at a marginal level of significance (Pϭ0.07). Also, a significantly (Pϭ0.03) greater decrease in apoA1 content of postprandial HDL was recorded after the unused fat meal. The peroxide content of LDL tended to decrease after the used fat meal and tended to increase after the control meal. These changes were significantly (Pϭ0.04) different. Susceptibility of isolated LDL to copper ion oxidation and plasma levels of malondialdehyde were unchanged during the study. These data suggest that in the postprandial period after a meal rich in used cooking fat, the enzymatic protection of LDL against accumulation of peroxides and atherogenic oxidative modification may be reduced, possibly due to factors associated with apoA1, without acutely affecting the intrinsic resistance of LDL to in vitro oxidation.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Reduced Postprandial Serum Paraoxonase Activity After a Meal Rich in Used Cooking Fat

Sutherland

Walker

Jong

et al. 1999

ATVB

120

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“…A small part of the ingested AL is accumulated in the rat liver (13), and the AL contains some kinds of xenobiotics such as aldehydes DRUG METABOLIC CHANGES BY AL DOSE LEVELS 105 including malonaldehydes, hydroperoxy alkenals, hydroperoxy epoxides, and polymers. It is well known that cytochrome P-450 content and drug-metabolizing activity are induced by ingesting lyophilic xenobiotics, and also there have been some reports (14-18) that both cytochrome P-450 and b5 act as peroxidase on lipid hydroperoxide.…”

Section: Discussionmentioning

confidence: 99%

Effects of dose levels of autoxidized linoleic acid on the drug-metabolizing system in rat liver.

Hiramatsu¹,

Kishida²,

Natake

1988

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryAutoxidized linoleic acid (AL) having 800meq/kg of per oxide value and 1,700meq/kg of carbonyl value was given in repeated oral doses at a daily dose of 0 (control)-7.5ml/kg to male Wistar rats for 5 successive days. The effect of increasing AL dose on the drug-metabolizing system was investigated in rat liver microsomes and S-9 fractions. All the rats of a daily dose of 5.0-7.5ml/kg died after the third day of consecutive oral doses. The cytochrome P-450 and b5 contents, enzyme activities in electron transfer system, aminopyrin-N-demethylase activity and S-9 activity (metabolic activation of 2-acetylaminofluorene) in the drug metabolizing system changed essentially in a similar manner, that is, both the contents and the activities were increased by a small dose of AL, and were decreased by a large dose of AL. These results strongly supported the fi ndings in a previous report wherein we observed the periodical effect of AL dose on the drug-metabolizing system. Key Words autoxidized linoleic acid, microsome, cytochrome P-450, cytochrome b5, electron transfer system, drug-metabolizing activity, aminopyrin-N-demethylase, S-9 activity, membrane disorder There have been some incompatible findings on the effect of autoxidized oil on drug-metabolizing activity in rat liver microsomes under the respective experimental conditions (1, 2). Therefore, in our previous work (3), AL having 800meq/kg of peroxide value and 1,700meq/kg of carbonyl value was administered periodically for 1-15 days at a daily dose of 2.5ml/kg body weight to male Wistar rats, and the effect of AL on the drug-metabolizing system in rat liver microsomes was studied. The cytochrome P-450 and b5 contents were increased by consecutive oral doses of AL for 3-7 days, then the amount of cytochrome P-450 decreased gradually, but the b5 decreased slightly. Thus, after administration for 11-15 days, the cytochrome P -450 content was significantly lower, but the cytochrome b5 content was rather high 97

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“…The fate of secondary products in the gastrointestinal lumen is made clear by the tracer experiments (4,14). When the radioactive secondary products were orally administered, radioactivity in the stomach quickly decreased and it increased in small intestines with time.…”

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confidence: 99%

The effects of orally administered linoleic acid and its autoxidation products on intestinal mucosa in rat.

Kanazawa¹,

Ashida

Minamoto

et al. 1988

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryLinoleic acid and its autoxidation products , hydroperoxides and their secondary products, were orally administered to rats (350mg each/rat). Hemorrhage was seen in the alimentary canal at 6h after the dose of hydroperoxides. To examine their toxicities on intestinal mucosa , the activities of mucous enzymes (sucrase, maltase , and alkaline phos phatase) were measured. Hydroperoxides and their secondary products decreased the enzyme activities in jejunum at 6h after the doses and increased them in both jejunum and ileum at 15h, as compared to linoleic acid or saline solution. The decrease of enzyme activity was marked in the hydroperoxide group and the increase was marked in the secondary product group. Then, in in vitro experiments, the effects of autoxidation products on these enzymes were determined. Autoxidation products inactivated only alkaline phosphatase . Thus, the results in vivo disagreed with those in vitro. It was assumed that autoxidation products orally administered attacked a membrane of intestinal microvilli whereas in vitro they directly affected the enzymes .

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Uptake of secondary autoxidation products of linoleic acid by the rat

Cited by 105 publications

References 37 publications

Reduced Postprandial Serum Paraoxonase Activity After a Meal Rich in Used Cooking Fat

Reduced Postprandial Serum Paraoxonase Activity After a Meal Rich in Used Cooking Fat

Effects of dose levels of autoxidized linoleic acid on the drug-metabolizing system in rat liver.

The effects of orally administered linoleic acid and its autoxidation products on intestinal mucosa in rat.

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