Uptake, functionality, and re-release of extracellular vesicle-encapsulated cargo

O’Brien, Killian P.; Ughetto, Stefano; Mahjoum, Shadi; Nair, Anil V.; Breakefield, Xandra O.

doi:10.1016/j.celrep.2022.110651

Cited by 89 publications

(81 citation statements)

References 61 publications

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“…In humans, Bennit et al showed that exosomes from WSU-DLCL2 (DLBCL) are favorably taken up by B cells and monocytes compared to T cells and NK cells, and these findings are in line with those of Hazan-Halevy et al [ 34 , 35 ]. The most prevalent mechanism of EV internalization is endocytosis, which releases the EV cargo into the recipient cell cytosol [ 36 , 37 ]. However, EVs may change recipient cell functionality without endocytosis via transfer receptors or molecule-activated T cell receptors [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Canine B Cell Lymphoma- and Leukemia-Derived Extracellular Vesicles Moderate Differentiation and Cytokine Production of T and B Cells In Vitro

Żmigrodzka

Pingwara

Pawlak

et al. 2022

IJMS

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Extracellular vesicles (EVs) are formed in physiological and pathological conditions by almost all mammalian cells. They are known as submicron “molecules” that transport and horizontally transfer their cargo from maternal cells to donor cells. Moreover, cancer cells produce tumor-derived EVs (TEVs), which are present in blood of patients with solid tumors and those with hematological malignancies. Their role in evading immune system surveillance and induction of immunosuppression in hematological cancer is limited. According to the authors’ best knowledge, there is no information about the impact of TEVs from canine lymphoma (CLBL-1) and leukemia (CLB70) on lymphocytes isolated from peripheral blood mononuclear cells (PBMCs). In conclusion, we demonstrate in in vitro experiments that CLBL-1 EVs and CLB70 EVs are effectively taken up by T and B lymphocytes. TEVs decrease the percentage of B lymphocytes and increase that of T lymphocytes, and change T cells’ phenotype into the effector memory (EM) or terminally differentiated effector memory (TEMRA) subtype after in vitro co-culturing. Moreover, CLBL70 EVs have pro-tumorogenic properties by inhibiting the production of CD8+IL-17+ cells.

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Section: Discussionmentioning

confidence: 99%

Canine B Cell Lymphoma- and Leukemia-Derived Extracellular Vesicles Moderate Differentiation and Cytokine Production of T and B Cells In Vitro

Żmigrodzka

Pingwara

Pawlak

et al. 2022

IJMS

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“…The caveat of these studies is that they were only performed in vitro , and not in vivo [ 184 ]. To add to the complexity, a recent study showed that cellular uptake does not automatically indicate integration into the cytosol; the exosomes can be re-released without apparent modification or housed in endosomes for days only to undergo degradation [ 185 ].…”

Section: Bioengineering Evsmentioning

confidence: 99%

Extracellular vesicles in β cell biology: Role of lipids in vesicle biogenesis, cargo, and intercellular signaling

Aguirre

Kulkarni

Becker

et al. 2022

Molecular Metabolism

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“…Unfortunately, uptake does not necessarily translate into successful delivery of EV-cargo and, despite the progress in understanding how EVs enter the cells, the fate of these EVs and their cargo remains unknown. Recent studies have attempted to analyze what happens to EV-associated molecules inside the target cells, employing strategies that allow the monitoring of EV-cargo [ 48 , 49 , 50 ]. Notably, one appealing approach in this direction is to translate to EV research knowledge on the mechanisms adopted by viruses.…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Extracellular Vesicles in Myeloid Neoplasms

Karantanou

Minciacchi

Karantanos

2022

IJMS

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Myeloid neoplasms arise from malignant primitive cells, which exhibit growth advantage within the bone marrow microenvironment (BMM). The interaction between these malignant cells and BMM cells is critical for the progression of these diseases. Extracellular vesicles (EVs) are lipid bound vesicles secreted into the extracellular space and involved in intercellular communication. Recent studies have described RNA and protein alterations in EVs isolated from myeloid neoplasm patients compared to healthy controls. The altered expression of various micro-RNAs is the best-described feature of EVs of these patients. Some of these micro-RNAs induce growth-related pathways such as AKT/mTOR and promote the acquisition of stem cell-like features by malignant cells. Another well-described characteristic of EVs in myeloid neoplasms is their ability to suppress healthy hematopoiesis either via direct effect on healthy CD34+ cells or via alteration of the differentiation of BMM cells. These results support a role of EVs in the pathogenesis of myeloid neoplasms. mainly through mediating the interaction between malignant and BMM cells, and warrant further study to better understand their biology. In this review, we describe the reported alterations of EV composition in myeloid neoplasms and the recent discoveries supporting their involvement in the development and progression of these diseases.

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Uptake, functionality, and re-release of extracellular vesicle-encapsulated cargo

Cited by 89 publications

References 61 publications

Canine B Cell Lymphoma- and Leukemia-Derived Extracellular Vesicles Moderate Differentiation and Cytokine Production of T and B Cells In Vitro

Canine B Cell Lymphoma- and Leukemia-Derived Extracellular Vesicles Moderate Differentiation and Cytokine Production of T and B Cells In Vitro

Extracellular vesicles in β cell biology: Role of lipids in vesicle biogenesis, cargo, and intercellular signaling

Extracellular Vesicles in Myeloid Neoplasms

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