Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish

Wang, Gongfa; Kang, Ning; Gong, Hongmei; Luo, Yan; Bai, Chenglian; Chen, Yuanhong; Ji, Xiang‐Shan; Huang, Changjiang; Dong, Qian

doi:10.1016/j.cryobiol.2015.08.016

Cited by 15 publications

(4 citation statements)

References 47 publications

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“…In this study, the results demonstrate that UCP2 plays a pivotal rolein ROS scavenging in human spermatozoa, and thus affects spermatozoa motility. Recently, a research group observed similar effects of UCP2 expression in the spermatozoa of zebrafish and yellow catfish [22,23]. It was found that a UCP2 promoter significantly improved the viability of post-thaw spermatozoa, accompanied by the decreased levels of ROS and MDA [23].…”

Section: Discussionmentioning

confidence: 77%

“…However, the function of UCP2 in male reproduction remains unclear. Recently, a research group found expression of UCP2 in the spermatozoa of zebrafish and yellow catfish and revealed the close relevance between the expression of UCP2 and spermatozoa ROS level, degree of oxidative damage, and post-thaw viability [22,23]. In human spermatozoa, the transcription of UCP2-encoding gene has been observed by quantifying mRNA,however, the UCP2 protein level was not further measured in the same study [24].…”

Section: Introductionmentioning

confidence: 99%

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UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

Wang

Hua

Huang

et al. 2018

Cell Physiol Biochem

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Background/Aims: To demonstrate the function of uncoupling protein 2 (UCP2) in the regulation of human spermatozoa motility. Methods: Semen samples were collected from donors with either normal spermatozoa motility (normospermia [NS]) or poor spermatozoa motility (asthenospermia [AS]). UCP2 protein in spermatozoawas quantified by Western blotting. The level of mitochondrial reactive oxygen species (mROS) was evaluated by MitoSOX Red. The activity of mitochondrial membrane potential (MMP) in spermatozoa was evaluated by a JC-1 assay and the ATP level was monitored by a luciferin-luciferase assay. Results: UCP2 was expressed in both NS and AS groups, with the former exhibiting a higher level than the latter. Immunofluorescence analysis shows that UCP2 is mainly located at the mid-region of human spermatozoa. The inhibition of UCP2 by a highly selective inhibitor, Genipin, results in not only impaired spermatozoa mobility (P<.05) but also an elevated level of mROS (P<.05), suggesting that UCP2 is involved in the maintenance of the spermatozoa mobility, which probably is achieved by promoting mROS elimination. Furthermore, H₂O₂ treatment of spermatozoa increases the mROS level coupled with the loss of spermatozoa mobility. Unexpectedly, this treatment also has a positive impact on the expression of UCP2 within a certain range of supplemental H₂O₂, indicating the moderate mROS level possibly serves as a feedback signal to stimulate the expression of UCP2. Finally, the treatment of spermatozoa by an ROS scavenger, N-acetyl-l-cysteine (NAC),decreases the level of mROS and increases the curvilinear velocity (VCL) of spermatozoa, but the UCP2 level is not affected. Conclusion: These results suggest an UCP2–mROS–motility regulatory system exists for maintaining spermatozoa mobility in humans. In such a system, UCP2 fulfills its function by promoting mROS elimination, and slightly over-produced mROS in turn serves as a signal to stimulates the expression of UCP2. This regulatory system represents a new potential target for the discovery of novel pharmaceuticals for the treatment of patients with low spermatozoa motility.

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

Wang

Hua

Huang

et al. 2018

Cell Physiol Biochem

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“…The signi cance of these ndings lies in the prospect of establishing germplasm banks from deceased animals up to the pre-rigor mortis stage, without compromising sperm quality. For the cryopreservation of zebra sh sperm, ensuring that the motility of a fresh sample falls within the range of 80-95% is minimally essential [31,32]. In this regard, sperm motility analyses yielding results similar to those in the control sample demonstrate the potential utility of this objective.…”

Section: Discussionmentioning

confidence: 96%

Understanding rigor mortis impacts on fish gamete viability

Dantas,

Marques,

Freitas

et al. 2023

Preprint

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This study aimed to evaluate the viability of reproductive gametes in zebrafish (Danio rerio), at different rigor mortis stages. Viability assessments were conducted on oocytes at various developmental stages using LIVE/DEAD and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. For sperm evaluation, both kinetic (CASA) and morphological assessments (Rose Bengal staining) were performed. Results demonstrated that rigor mortis progression significantly impacted oocyte viability during post-rigor stages, with the following viability rates: pre-rigor (70.43 ± 12.31%), fresh/control (46.43 ± 12.54%), post-rigor (27.62 ± 22.29%), and rigor mortis (comparable to fresh/control). Conversely, sperm kinetics exhibited nuanced responses to the rigor mortis stages, with specific parameters showing sensitivity, whereas the others remained relatively stable. Sperm motility was higher in the fresh/control (63.23 ± 19.03%) and pre-rigor (58.96 ± 14.38%) compared to the post-rigor group (3.34 ± 4.65%). This study highlights the significance of the pre-rigor for successful gamete collection and preservation. These findings provide valuable insights for conservation efforts and optimization of genetic resource management for endangered fish species. This study aimed to develop effective assistive reproductive techniques by elucidating the interplay between rigor mortis and gamete quality, contributing to the broader goals of species conservation and maintenance of genetic diversity in fish populations.

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“…In humans and fish, UCP2 plays a protective role in maintaining sperm viability 54,55 . In addition, the expression level of UCP2 was positively correlated with the enhancement of the energy metabolism (glycolysis) pathway and the increase in glucose‐derived pyruvate levels 56,57 .…”

Section: Discussionmentioning

confidence: 99%

Impaired fertility in 4930590J08Rik mutant male mice is associated with defective sperm energy metabolism

Chen

et al. 2022

The FASEB Journal

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Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish

Cited by 15 publications

References 47 publications

UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

UCP2 Mitigates the Loss of Human Spermatozoa Motility by Promoting mROS Elimination

Understanding rigor mortis impacts on fish gamete viability

Impaired fertility in 4930590J08Rik mutant male mice is associated with defective sperm energy metabolism

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