Upregulation of long noncoding RNA PVT1 predicts unfavorable prognosis in patients with clear cell renal cell carcinoma

Bao, Xu; Duan, Junyao; Yan, Ye; Ma, Xin; Zhang, Yu; Wang, Hanfeng; Ni, Dong; Wu, Shengpan; Peng, Cheng; Yang, Fan; Gao, Yu; Li, Xintao; Chen, Jianwen; Du, Qingshan; Zhang, Fan; Zhang, Xu

doi:10.3233/cbm-170251

Cited by 28 publications

(33 citation statements)

References 66 publications

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“…One major finding of our study is that high PVT1 expression is tightly associated with several clinical parameters and a poor prognosis in SCCHN patients, which is consistent with reports in other solid tumors including non-small-cell lung cancer 10 , 12 , hepatocellular carcinoma 15 , clear renal cell carcinoma 35 , prostate cancer 17 , glioma 36 , 37 , gastric 23 and cervical cancer 38 , etc. We have to mention that our current clinical analysis is based on a relative small sample size, therefore, verification in a larger patient cohort will further strengthen the clinical significance of PVT1 in SCCHN patients.…”

Section: Discussionsupporting

confidence: 90%

LncRNA PVT1 promotes malignant progression in squamous cell carcinoma of the head and neck

Yu¹,

Wang

Guo

et al. 2018

J. Cancer

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Long non-coding RNAs (lncRNAs) are potentially critical regulators of cancer malignant behaviours. Aberrant expression and dysfunction of lncRNA PVT1 have been reported in multiple human cancers. However, its role in squamous cell carcinoma of the head and neck (SCCHN) remains largely unknown. Our current study demonstrated that PVT1 expression was increased in SCCHN. High PVT1 expression was positively correlated with SCCHN clinical parameters including T classification, clinical stages and cervical lymph node metastasis. More importantly, high PVT1 expression predicted a poor prognosis in SCCHN patients. Gain-of function and loss-of function studies further indicated that PVT1 promoted the proliferation and invasion of SCCHN both in vitro and in vivo, which was accompanied by epithelial-mesenchymal transition and enhanced cancer stem cell-like properties. Further mechanistic investigation revealed that PVT1 activated Wnt/β-catenin signalling pathway, and inhibition of Wnt/β-catenin signalling reversed the malignant progression caused by PVT1 overexpression. Together, our study reveals that PVT1 accelerates the malignant progression of SCCHN and represents a potential biomarker and therapeutic target in SCCHN.

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Section: Discussionsupporting

confidence: 90%

LncRNA PVT1 promotes malignant progression in squamous cell carcinoma of the head and neck

Yu¹,

Wang

Guo

et al. 2018

J. Cancer

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“…Consistent with our findings, it has been reported that LINC01094 plays its pro-tumor role in the development of ccRCC by regulating the miR-224-5p/CHSY1 axis ( Jiang et al, 2020 ). Whilst, lncRNA PVT1 has been indicated to function as a tumor promoter that has a significant role in the metastasis in ccRCC ( Bao et al, 2017 ). Moreover, Jiang et al (2020) have reported that LINC01094 was activated by FOXM1 at the transcriptional level and their experimental indicated that LINC01094 acts as a sponge of miR-224-5p while CHSY1 was a miR-224-5p-targeted mRNA indicating the potential value of miR-224-5p/CHSY1 regulatory axis.…”

Section: Discussionmentioning

confidence: 99%

Long Non-coding RNA LINC01094 Promotes the Development of Clear Cell Renal Cell Carcinoma by Upregulating SLC2A3 via MicroRNA-184

Wang

et al. 2020

Front. Genet.

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Clear cell renal cell carcinoma (ccRCC) is the most common subtype of RCC. Compelling evidence has highlighted the crucial role of long non-coding RNA (lncRNA) in ccRCC. Our current study aims to explore the regulatory mechanism of LINC01094 in the development of ccRCC. Dual-luciferase reporter experiment verified the targeting relationship among miR-184, LINC01094, and SLC2A3. Furthermore, the interaction between LINC01094 and miR-184 was confirmed by RNA immunoprecipitation (RIP) and RNA pull-down. Biological behaviors of ccRCC cells were investigated through cell counting kit-8 (CCK8), scratch test, Transwell, and flow cytometry. The effect of SLC2A3 on the tumorigenicity of nude mice was evaluated in vivo . In ccRCC cells and clinical tissues, LINC01094 and SLC2A3 were highly expressed while miR-184 was lowly expressed. Besides, miR-184 was verified to be a direct target of LINC01094. Silencing LINC01094, up-regulating miR-184, or reducing SLC2A3 inhibited the growth, migration, and invasion of ccRCC cells. Tumor growth was suppressed by silenced LINC01215 via reducing the expression of SLC2A3 via miR-184. Taken together, silencing LINC01094 inhibited SLC2A3 expression by up-regulating miR-184, thereby inhibiting the development of ccRCC.

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“…Due to the advances of high-throughout technology, numerous lncRNAs have been detected and profiled and many of them participated in RCC progression. For example, lncRNA PVT1 was shown to be upregulated in ccRCC, which was associated with poor outcome [10] due to its promotion of cell proliferation and invasion by competitively binding with miR-200 family through regulating the expression of metastasis related genes [11]. Similarly, Xu et al identified that increased lncRNA PANDAR expression correlated with an advanced TNM stage and with lymph node involvement and distant metastasis in ccRCC and PANDAR downregulation induced cell cycle arrest and apoptosis in RCC cells [5].…”

Section: Introductionmentioning

confidence: 99%

LncRNA TP73-AS1 Promotes Cell Proliferation and Inhibits Cell Apoptosis in Clear Cell Renal Cell Carcinoma Through Repressing KISS1 Expression and Inactivation of PI3K/Akt/mTOR Signaling Pathway

Liu¹,

Zhao²,

Zhou³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Emerging evidence suggests that long non-coding RNAs (lncRNAs) play a vital regulatory role in the pathogenesis and progression of renal cell carcinoma (RCC). We aim to determine lncRNA profiles in clear cell RCC (ccRCC) and investigate key lncRNAs involved in ccRCC tumorigenesis and progression. Methods: RNA sequencing technique and qPCR were used to determine the candidate lncRNAs in ccRCC tissues. The correlations between lncRNA P73 antisense RNA 1T (TP73-AS1) levels and survival outcomes were analyzed to elucidate its clinical significance. The underlying mechanisms of TP73-AS1 in ccRCC were analyzed through in vitro functional assays. Results: We found TP73-AS1 was upregulated in 40 ccRCC tissues compared with adjacent normal renal tissues and increased TP73-AS1 was correlated to aggressive clinicopathologic features and unfavorable prognosis. Knockdown of TP73-AS1 suppressed cell proliferation, invasion and induced cell apoptosis. We also identified KISS-1 metastasis-suppressor (KISS1) was significantly upregulated in TP73-AS1 knockdown cells. Further, we revealed that TP73-AS1 suppressed KISS1 expression through the interaction with Enhancer of zeste homolog 2 (EZH2) and the specific binding to KISS1 gene promoter region. Knockdown of KISS1 partly reversed TP73-AS1 knockdown-induced inhibition of cell proliferation and promotion of apoptosis. We further determined that TP73-AS1 knockdown activated PI3K/Akt/mTOR signaling pathway, while overexpression of TP73-AS1 induced inhibition of PI3K/Akt/mTOR pathway and these effects could be partly abolished by overexpression of KISS1. Conclusion: In conclusion, we identified that TP73-AS1 as an oncogenic lncRNA in the development of ccRCC and a potential target for human renal carcinoma treatment.

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Upregulation of long noncoding RNA PVT1 predicts unfavorable prognosis in patients with clear cell renal cell carcinoma

Abstract: PVT1 may be an oncogene as well as may promote metastasis in ccRCC and could serve as a potential biomarker to predict the prognosis of ccRCC patients.

Cited by 28 publications

References 66 publications

LncRNA PVT1 promotes malignant progression in squamous cell carcinoma of the head and neck

LncRNA PVT1 promotes malignant progression in squamous cell carcinoma of the head and neck

Long Non-coding RNA LINC01094 Promotes the Development of Clear Cell Renal Cell Carcinoma by Upregulating SLC2A3 via MicroRNA-184

LncRNA TP73-AS1 Promotes Cell Proliferation and Inhibits Cell Apoptosis in Clear Cell Renal Cell Carcinoma Through Repressing KISS1 Expression and Inactivation of PI3K/Akt/mTOR Signaling Pathway

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