Upregulated microRNA‑671‑3p promotes tumor progression by suppressing forkhead box P2 expression in non‑small‑cell lung cancer

Abstract

In the present study, the expression of microRNA (miR)-671-3p in non-small-cell lung cancer (NSCLC) was detected via reverse transcription-quantitative polymerase chain reaction analysis, and its role in cell proliferation, apoptosis, migration and invasion was investigated via Cell Counting Kit-8, colony formation, flow cytometry, Transwell and scratch assays, respectively. It was observed that the expression of miR-671-3p was upregulated in NSCLC tissues and cell lines (A549 and H1975). Treatment with miR-671-3p inhibitors suppressed cell proliferation, migration and invasion, and increased apoptosis in vitro, suggesting that miR-671-3p functions as an oncogene in NSCLC. In addition, forkhead box P2 (FOXP2) has been reported to be a tumor suppressor that is downregulated in several types of cancer, and its low expression was confirmed in NSCLC tissues and cell lines in the current study via western blotting. The results of the luciferase reporter assay also demonstrated that miR-671-3p targeted directly the 3′-untranslated region of FOXP2. Furthermore, overexpression of FOXP2 in A549 and H1975 cell lines suppressed the growth, migration and invasion, and promoted apoptosis, whereas these effects were reversed by transfection with miR-671-3p mimics, suggesting that miR-671-3p promoted tumor progression via regulating FOXP2. Taken together, the results reported in the present study implied that miR-671-3p may be a potential therapeutic target in NSCLC.

<p>Hsa_circ_0043265 Suppresses Proliferation, Metastasis, EMT and Promotes Apoptosis in Non-Small Cell Lung Cancer Through miR-25-3p/FOXP2 Pathway</p>

“…30 In NSCLC, FOXP2 expression was also considered negatively correlated with the progression of NSCLC. 26 Consistent with previous studies, we also found that FOXP2 expression was decreased in Figure 6 Effects of FOXP2 silencing on the progression of NSCLC cells. A549 and NCI-H23 cells were co-transfected with hsa_circ_0043265 overexpression plasmid and si-FOXP2 or their negative controls (circ-NC and scramble).…”

“…25 Li et al determined that FOXP2 was downregulated in NSCLC and could act as a target of miR-671-3p to participate in the regulation of miR-671-3p on the progression of NSCLC. 26 Therefore, FOXP2 might act as an important negative regulator of NSCLC progression.…”

Identification of Acute Myeloid Leukemia Bone Marrow Circulating MicroRNAs

“…Our patient group comparison at diagnosis based on their overall survival showed that miR-328, miR-671-3p, miR-636 and miR-363 are upregulated in relapsed patients. These miRs are described in the literature as promising prognostic biomarkers in AML or other malignant diseases [ 21 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 ]. Interestingly, all the patients involved in this comparison were not grafted, which suggests that these four miRs could constitute a signature to guide the choice of the best treatment strategy, especially the utility of grafting patients at diagnosis.…”

Use of signals of positive and negative selection to distinguish cancer genes and passenger genes

“…It acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3'-UTR. ZC3H13 has been shown to serve as a tumor suppressor in colorectal cancer (Zhu et al, 2019). Zhu D,Zhou J,Zhao J,Jiang G,Zhang X,Zhang Y,Dong M. ZC3H13 suppresses colorectal cancer proliferation and invasion via inactivating Ras-ERK signaling.…”

“…Numerous recent studies indicate a tumor suppressor like role for FOXP2 (Campbell et al, 2010;Cuiffo et al, 2014;Yan et al, 2015;Diao et al, 2018;Song et al, 2017;Li et al, 2019), others present evidence for an oncogene-like role of the protein (Campbell et al, 2010;Zhong et al, 2017;Wu et al, 2018;.…”