Upregulated LINC01088 facilitates malignant phenotypes and immune escape of colorectal cancer by regulating microRNAs/G3BP1/PD-L1 axis

Li, Chenmeng; Pan, Bei; Wang, Xuhong; Liu, Xiangxiang; Qin, Jian; Gao, Tianyi; Sun, Huiling; Pan, Yuqin; Wang, Shukui

doi:10.1007/s00432-022-03981-8

Cited by 15 publications

(18 citation statements)

References 43 publications

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“…By suppressing p21 by binding to EZH2, LINC01088 can enhance cell proliferation, ultimately aggravating the tumorigenicity of non-small cell lung cancer (NSCLC) [ 35 ]. LINC01088 directly targets miR-548b-5p and miR-548c-5p, as well as enhances the expression of G3BP1 and PD-L1, consequently boosting the development of colorectal cancer and immune evasion [ 36 ]. In addition, among central nervous system tumors, LINC01088 exerts pro-oncogenic activities in glioma via binding to SNRPA and regulating SNRPA mRNA transcription [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Identifying Oxidized Lipid Metabolism-Related LncRNAs as Prognostic Biomarkers of Head and Neck Squamous Cell Carcinoma

Zhang

Kim

Han

et al. 2023

JPM

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The relationship between oxidized lipid metabolism and the immunological function of cancer is well known. However, the functions and regulatory mechanisms of lncRNAs associated with oxidized lipid metabolism in head and neck squamous cell carcinoma (HNSCC) remain to be fully elucidated. In this study, we established an oxidized lipid metabolism-related lncRNA prognostic signature to assess the prognosis and immune infiltration of HNSCC patients. The HNSCC transcriptome was obtained from The Cancer Genome Atlas. The choice of the target genes with a relevance score greater than 10 was performed via a correlation analysis by GeneCards. Patients were categorized by risk score and generated with multivariate Cox regression, which was then validated and evaluated using the Kaplan–Meier analysis and time-dependent receiver operating characteristics (ROC). A nomogram was constructed by combining the risk score with the clinical data. We constructed a risk score with 24 oxidized lipid metabolism-related lncRNAs. The areas’ 1-, 2-, and 3-year OS under the ROC curve (AUC) were 0.765, 0.724, and 0.724, respectively. Furthermore, the nomogram clearly distinguished the survival probabilities of patients in high- and low-risk groups, between which substantial variations were revealed by immune infiltration analysis. The results supported the fact that oxidized lipid metabolism-related lncRNAs might predict prognoses and assist with differentiating amid differences in immune infiltration in HNSCC.

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Section: Discussionmentioning

confidence: 99%

Identifying Oxidized Lipid Metabolism-Related LncRNAs as Prognostic Biomarkers of Head and Neck Squamous Cell Carcinoma

Zhang

Kim

Han

et al. 2023

JPM

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“…According to the sum of the correlations of these 385 genes in gastrointestinal cancer, we screened the 30 genes with the strongest correlations, which are RARS1, BTF3, HSPA4, NSA2, HNRNPA1, EIF3M, CCT4, OLA1, UIMC1, G3BP1, NACA, RSL1D1, ETF1, MRPS27, RBM22, CCT8, GEMIN5, LARS1, PA2G4, CDK7, NIFK, RSL24D1, EIF3E, THG1L, INTS13, UBE2D2, RPS23, DIMT1, RIOK2, and TTC27 respectively. Previous studies have reported that overexpression of BTF3, CCT8, CDK7, ELF3M, G3BP1, HSPA4, OLA1, and RSL1D1 can contribute to the occurrence and development of CRC ( Goh et al, 2011 ; Zhou et al, 2019 ; Zhou et al, 2021b ; Liao et al, 2021 ; Zhang et al, 2021 ; Liu et al, 2022a ; Liu et al, 2022b ; Li et al, 2022 ), overexpression of CCT4 and PA2G4 can contribute to the occurrence and development of LIHC( Li et al, 2021a ; Sun et al, 2022 ), overexpression of DIMT1 and HNRNPA1 can contribute to the occurrence and development of STAD ( Liu et al, 2017 ; Zhu et al, 2022 ), and overexpression of EIF3E and UIMC1 can contribute to the ESCA occurrence and development ( Xu et al, 2018 ; Yang et al, 2018 ). Therefore, we believe that these genes have the potential to be called diagnostic and therapeutic targets of gastrointestinal cancer.…”

Section: Discussionmentioning

confidence: 99%

Nucleophosmin 1 is a prognostic marker of gastrointestinal cancer and is associated with m6A and cuproptosis

Liu

Liu²,

Zeng³

et al. 2022

Front. Pharmacol.

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Background: NPM1 is highly expressed in a variety of solid tumors and promotes tumor development. However, there are few comprehensive studies on NPM1 analysis in gastrointestinal cancer.Methods: We used bioinformatics tools to study the expression difference of NPM1 between gastrointestinal cancer and control group, and analyzed the relationship between its expression level and the diagnosis, prognosis, functional signaling pathway, immune infiltration, m6A and cuproptosis related genes of gastrointestinal cancer. At the same time, the expression difference of NPM1 between esophageal carcinoma (ESCA) samples and control samples was verified by in vitro experiments.Results: NPM1 was overexpressed in gastrointestinal cancer. In vitro experiments confirmed that the expression of NPM1 in ESCA samples was higher than that in normal samples. The expression of NPM1 has high accuracy in predicting the outcome of gastrointestinal cancer. The expression of NPM1 is closely related to the prognosis of multiple gastrointestinal cancers. Go and KEGG enrichment analysis showed that NPM1 co-expressed genes involved in a variety of biological functions. NPM1 expression is potentially associated with a variety of immune cell infiltration, m6A and cuproptosis related genes in gastrointestinal cancers.Conclusion: NPM1 can be used as a diagnostic and prognostic marker of gastrointestinal cancer, which is related to the immune cell infiltration and the regulation of m6A and cuproptosis.

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“…The present research was approved by the Ethics Committee of the Second Affiliated Hospital of Nanchang University and abided by the Declaration of Helsinki. The informed consent was provided by the healthy donors [30].…”

Section: Isolation Identification and Culture Of Human Cd8 + T Cellsmentioning

confidence: 99%

“…MCF-7/FUL cells (1 × 10 5 cells/well) and activated CD8 + T cells (1 × 10 6 cells/well) were respectively placed in the outer and inner chambers of Transwell that contained DMEM with 10% human AB serum Hyclone (South Logan, UT, USA). Cells were cultured under conditions of 37°C and 5% CO2 [30].…”

Section: P R E P R I N Tmentioning

confidence: 99%

“…Subsequent to three PBS washes, CD8 + T cells co-cultured with MCF-7/FUL cells were subjected to 10-min fixing with 4% paraformaldehyde at room temperature and 20-min immersion in 0.1% crystal violet (Sigma-Aldrich) at 37°C. Finally, these cells were rinsed with running water and then photographed and observed under a microscope (Nikon E600, Nikon, Tokyo, Japan) [30].…”

Section: Crystal Violet Stainingmentioning

confidence: 99%

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PARP9 knockdown confers protection against chemoresistance and immune escape of breast cancer cells by blocking the PI3K/AKT pathway

Hong

Dong

et al. 2023

Arch Med Sci

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IntroductionThis study probes the mechanism of the PARP9/PI3K/AKT/PD-L1 axis in the chemoresistance and immune escape of breast cancer (BRCA) cells.Material and methodsThe expression of related genes was detected in MCF-7/FUL cells. After MCF-7/FUL cells were treated with sh-PARP9 and/or the PI3K/AKT pathway activator, drug resistance, proliferation, migration, invasion, and apoptosis were measured. Afterward, MCF-7/FUL cells were co-cultured with CD8+ T cells for examining the positive rate and density of MCF-7/FUL cells, the percentage and apoptosis of CD8+ T cells, and the expression of immune-related factors in cell supernatants. Nude mice were subcutaneously injected with sh-PARP9-transfected MCF-7/FUL cells for in vivo validation.ResultsPARP9 was highly expressed in MCF-7/FUL cells. Sh-PARP9 transfection suppressed cell migration, proliferation, and invasion while accelerating apoptosis in MCF-7/FUL cells, accompanied by downregulated PD-L1, p-PI3K, and p-AKT expression, and reduced IC50 and FUL resistance. After co-culture of MCF-7/FUL cells with CD8+ T cells, the percentage of CD8+ T cells, the expression of immune-related factors in supernatants, and the positive rate of MCF-7/FUL cells increased, while the apoptosis of CD8+ T cells and the density of adherent MCF-7/FUL cells were diminished. These trends were negated by further activating the PI3K/AKT pathway. PARP9 knockdown suppressed xenograft growth, decreased p-PI3K, p-AKT, PD-L1, and Cyclin D1 expression, and augmented p-Cdc2 and cleaved caspase 3 levels in nude mice.ConclusionsPARP9 knockdown blocked the PI3K/AKT pathway to downregulate PD-L1, thus depressing chemoresistance and immune escape in BRCA.

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Upregulated LINC01088 facilitates malignant phenotypes and immune escape of colorectal cancer by regulating microRNAs/G3BP1/PD-L1 axis

Cited by 15 publications

References 43 publications

Identifying Oxidized Lipid Metabolism-Related LncRNAs as Prognostic Biomarkers of Head and Neck Squamous Cell Carcinoma

Identifying Oxidized Lipid Metabolism-Related LncRNAs as Prognostic Biomarkers of Head and Neck Squamous Cell Carcinoma

Nucleophosmin 1 is a prognostic marker of gastrointestinal cancer and is associated with m6A and cuproptosis

PARP9 knockdown confers protection against chemoresistance and immune escape of breast cancer cells by blocking the PI3K/AKT pathway

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