UPLC MS/MS Profile and Antioxidant Activities from Nonpolar Fraction of Patiwala (Lantana camara) Leaves Extract

Abstract

One of the plants used in Indonesian traditional medicine, namely, Patiwala (Lantana camara), is traditionally used to treat some diseases, including itching, wounds, ulcers, swelling, eczema, tetanus, malaria, tumors, rheumatism, and headaches. This study aimed to characterize the compound nonpolar fraction of Patiwala leaf capable of scavenging free radicals. The characterization of compound was carried out using the Ultra-Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) with positive ion method, while the antioxidant testing was carried out using the radical DPPH (2,2-diphenyl-1-picrylhidrazyl) and FRAP (ferric reducing antioxidant power) methods. The results showed that the nonpolar fraction of the methanol extract of L. camara leaves was very strong toward DPPH radicals (IC50 34.65 ± 1.26 μg/mL and 40.23 ± 0.18 μg/mL), and FRAP radical (IC50 4.93 ± 0.22 μg/mL and 12.79 ± 0.09 μg/mL). Nineteen compounds identified by UPLC-MS/MS method were Resveratrol dimer, iso-humolones, oleuropein glucoside, quercetin-3-O-glycoside, myricetin, oleuropein, 12-deoxy-16-hydroxy-phorbol, aloeresin A, humulones, ursolic acid, viniferin, Epicatechin, oleanolic acid, 5-hydroxy-3′,4′,7-trimerthoxy-flavanone, Apigenin-6,8-di-C-β-D-glucoside, procyanidin A2, caffeoyl-O-hexoside, tansihnone IIA, and phillyrin. The methanolic extract of L. camara leaves can be developed as a source of antioxidants from natural ingredients.

In Vitro Evaluation and In Silico Calculations of the Antioxidant and Anti-Inflammatory Properties of Secondary Metabolites from Leonurus sibiricus L. Root Extracts

“…Although the BDE values are of the same order of magnitude for all radicals, they are slightly lower for flavonoids, indicating that they are more potent antioxidants than phenolic acids. This can be reasoned by the fact that flavonoids contain one more phenolic group, and as is known, their number affects the antioxidant activity [ 41 ]. The hydrogen is most easily detached from the 3′ and 4′-OH sites of flavonoids, which is in line with the experimental observations.…”

Efektivitas Pemberian Ekstrak Tanaman Komba-Komba (Lantana camara) terhadap Proses Penyembuhan Luka pada Mencit yang diberi Luka Insisi: Penelitian dengan Hewan Coba

“…Aktifitas antioksidan alkaloid terjadi melaui penghambatan proses NADPH-oksidase yang merupakan enzim utama pada pembentukan oksigen reaktif pada ditingkat sel (Dalimunthe et al, 2018;Macáková et al, 2019). Dari beberapa penelitian terhadap tumbuhan Lantana camara, teridentifikasi fraksi nonpolar dengan potensi kandungan senyawa flavanoid Miricetin,4', dan senyawa fenolik (Dimer resveratrol, Oleuropein glukosida, Isomer oleuropein glukosida, Aloeresin A, Humulon), dengan potensi antioksidan alami yang kuat menggunkana metode analisis DPPH dan FRAP (Ruslin et al, 2022).…”

Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)

“…In the case of derivatized ITCs, TFA was preferred to rather than formic acid, as it was lower the pH of the mobile phase and improved the width of the peaks. Improvement of the shape of the peaks was achieved by increasing the column temperature to 30°C rather than 20°C for GLs and polyphenolic compounds and to 35°C for ITCs as it was previously suggested by others (Pilipczuk et al, 2017;Ruslin et al, 2022). For the ionization of polyphenols, the electrospray ionization (ESI) with either negative or positive (ESI ± ) mode was used while GLs and derivatized ITCs were ionized under ESIand ESI + respectively.…”