Up, down and up again is a signature global gene expression pattern at the beginning of gymnosperm embryogenesis

Zyl, Leonel van; Bozhkov, Peter V.; Clapham, David; Sederoff, Ronald R.; Arnold, Sara von

doi:10.1016/s1567-133x(02)00068-6

Cited by 43 publications

(29 citation statements)

References 20 publications

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“…Two-dimensional gel electrophoresis-based proteomic approaches have been applied to investigate molecular changes during SE in several species (Choi and Sung, 1984;Gianazza et al, 1992;Reinbothe et al, 1992;Boyer et al, 1993;Pedroso et al, 1995;Yoshida et al, 1995;Dodeman and Ducreux, 1996;Giroux and Pauls, 1996;Dodeman et al, 1998;Sallandrouze et al, 1999) with limited success. Large-scale transcription analyses of embryogenesis have also been reported in several species (Giroux and Pauls, 1997;van Zyl et al, 2002van Zyl et al, , 2003Stasolla et al, 2003;Thibaud-Nissen et al, 2003;Stasolla et al, 2004).…”

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confidence: 96%

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

et al. 2005

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The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from the cultures at different time points (2, 5, and 8 weeks), separated by two-dimensional gel electrophoresis, and detected by silver staining. More than 2,000 proteins could be reproducibly resolved and detected on each gel. Statistical analysis showed that 54 protein spots were significantly (P , 0.05) changed in expression (accumulation) during the 8 weeks of culture, and most of these spots were extracted from colloidal Coomassie-stained two-dimensional gel electrophoresis gels and were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry or liquid chromatography-tandem mass spectrometry analysis. Using a publicly available expressed sequence tag database and the Mascot search engine, we were able to identify 16 differentially expressed proteins. More than 60% of the differentially expressed protein spots had very different patterns of gene expression between 2HA and Jemalong during the 8 weeks of culture.Plants must coordinate the growth of root and shoot meristems to maintain an appropriate balance of root and shoot organs and to respond and adapt to various environmental conditions. This balance is achieved by an intermeristem coordination of growth and development of the plant and involves the interplay of several long-range signals (Wopereis et al., 2000;Jiang and Gresshoff, 2002;Searle et al., 2003). Somatic (or asexual) embryogenesis (SE) is the process whereby somatic cells differentiate into embryos and ultimately into plants via a series of characteristic morphological stages, particularly the later stages, which resemble the zygotic stages of development (Zimmerman, 1993;Schmidt et al., 1997). SE is the developmental restructuring of somatic cells toward the embryogenic pathway and forms the basis of cellular totipotency in higher plants (Nolan et al., 2003;Imin et al., 2004). Two experimental approaches are available to examine this process in detail: (1) leaf cells grown in culture from protoplasts to form calli and, subsequently, the generation of embryos and then the development of plants (Rose and Nolan, 1995); and (2) leaf explants, which form calli in culture, and the concomitant production of embryos and vascular tissues. Depending on the plant system, auxin and/or cytokinin are required to enable embryogenesis to occur in culture (Schmidt et al., 1997;Somleva et al., 2000;Baudino et al., 2001;Hecht et al., 2001). In Medicago truncatula (Australian barrel medic), Nolan et al. (2003) found that embryogenesis required both auxin and cytokinin addition, although some embryos could form on cytokinin alone.The first appearance of embryos from mesophyll protoplasts occurs...

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confidence: 96%

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

et al. 2005

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“…Recently, we showed that arrays from P. taeda can be used for studying gene expression in Picea abies and Pinus sylvestris (van Zyl et al, 2002a(van Zyl et al, , 2002bStasolla et al, 2003). At present, the possibility of sampling single cells or a small number of cells, and also obtaining synchronized populations of cells, is restricted (Hertzberg et al, 2001;van Zyl et al, 2002a).…”

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confidence: 99%

Microarray Analyses of Gene Expression during Adventitious Root Development in Pinus contorta

et al. 2004

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In order to investigate the gene expression pattern during adventitious root development, RNA of Pinus contorta hypocotyls, pulse-treated with the auxin indole-3-butyric acid and harvested at distinct developmental time points of root development, was hybridized to microarrays containing 2,178 cDNAs from Pinus taeda. Over the period of observation of root development, the transcript levels of 220 genes changed significantly. During the root initiation phase, genes involved in cell replication and cell wall weakening and a transcript encoding a PINHEAD/ZWILLE-like protein were up-regulated, while genes related to auxin transport, photosynthesis, and cell wall synthesis were down-regulated. In addition, there were changes in transcript abundance of genes related to water stress. During the root meristem formation phase the transcript abundances of genes involved in auxin transport, auxin responsive transcription, and cell wall synthesis, and of a gene encoding a B-box zinc fingerlike protein, increased, while those encoding proteins involved in cell wall weakening decreased. Changes of transcript abundance of genes related to water stress during the root meristem formation and root formation phase indicate that the plant roots had become functional in water transport. Simultaneously, genes involved in auxin transport were up-regulated, while genes related to cell wall modification were down-regulated. Finally, during the root elongation phase down-regulation of transcripts encoding proteins involved in cell replication and stress occurred. Based on the observed changes in transcript abundances, we suggest hypotheses about the relative importance of various physiological processes during the auxin-induced development of roots in P. contorta.

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“…In addition, several members of the SAM metabolic pathways have been also found to be upregulated during early stages of cellular dedifferentiation before the establishment of somatic embryogenesis in cotton [108] and also during early embryogenesis in P. abies [109].…”

Section: Methylation Of Histone Proteinmentioning

confidence: 99%

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

2018

J App Biol Biotech

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Based on the previous available documents involving molecular events during plant somatic embryogenesis, this report aims to review the advances that have been made for the past several years in the area of molecular mechanism of plant somatic embryogenesis. To begin with, studies suggest that the induction and differentiation of embryos from somatic tissue directly or through callusing involves the interaction of various cellular and molecular factors. Several intra-and extra-cellular proteins such as germins and germins-like proteins, lipid transfer proteins, heatshock proteins, and late embryogenesis abundant proteins are known to regulate the induction of somatic embryos from the somatic cell. Simultaneously, regulation and expression of specific genes such as housekeeping genes OsIAA in rice; hormone-responsive genes Dcarg-1, Dchsp-1, DcECP31, DcEMB1 in carrot; and AtECP63, Mt somatic embryo-related factor 1 in arabidopsis have been identified to play key roles during the process of somatic embryogenesis. These genes are known to express differentially for synthesis of new proteins during induction and development of somatic embryo. In addition, several transcription factors such as leafy cotyledon genes, agamouslike15 (AGL15) gene, ethylene-responsive element-binding protein (EREBPs), knotted1-like homeobox proteins, and RWP-RK group of plant-specific transcription factors are equally known that efficiently control the molecular events of somatic embryogenesis. Further, it is also now established that epigenetic factors such asDNA methylation, histone deacetylation/methylation, and microRNAs also influence the molecular mechanism of plant somatic embryogenesis.

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Up, down and up again is a signature global gene expression pattern at the beginning of gymnosperm embryogenesis

Cited by 43 publications

References 20 publications

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

Microarray Analyses of Gene Expression during Adventitious Root Development in Pinus contorta

Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review

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