Unusual phenotype of <i>cis</i>‐AB

Cho, D.; Kee, Seung‐Jung; Shin, Jong Hee; Suh, Soon Pal; Ryang, Dong‐Wook

doi:10.1046/j.1423-0410.2003.00307_3.x

Cited by 11 publications

(13 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Weak or unusual expression of the B antigen featured in these three phenotypes, but, interestingly, two donors also demonstrated weak A expression. Our group has previously reported this unusual serological finding in another Korean individual with the cis‐AB01/A102 genotype [2]. The explanation for the weak A expression might lie in genetic loci outside the ABO allele; if, for example, these people also synthesized less H antigen, then perhaps this limitation would prevent the otherwise fully functional enzyme encoded by the A102 allele from producing the expected strong A expression.…”

Section: Resultsmentioning

confidence: 87%

The serological and genetic basis of the cis‐AB blood group in Korea

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 87%

The serological and genetic basis of the cis‐AB blood group in Korea

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…low expression of the B antigen is in contrast with other typical cis-AB subjects, and this can be due to allele competition in cases of cis-AB/O genotypes. As the phenotype of a ABO*cis-AB01/ ABO*A102 subject was previously observed as A1 phenotype multiple times, this lack of B antigen expression (phenotypically A1) is to be considered a not rare phenomenon in ABO*cis-AB01/ ABO*A102 subjects [29][30][31]. This observation can be explained by the fact that the A1 glycosyltransferase is in competition with that from cis-AB01 and does not leave enough H substrate to produce detectable B antigen.…”

Section: Discussionmentioning

confidence: 92%

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Chun

Ryu

Cha

et al. 2018

Transfus Med Hemother

View full text Add to dashboard Cite

Background: The cis-AB phenotype, although rare, is the relatively most frequent of ABO subgroups in Koreans. To prevent ABO mistyping of cis-AB samples, our hospital has applied a combination of the manual tile method with automated devices. Herein, we report cases of ABO mistyping detected by the combination testing system. Methods: Cases that showed discrepant results by automated devices and the manual tile method were evaluated. These samples were also tested by the standard tube method. The automated devices used in this study were a QWALYS-3 and Galileo NEO. Exons 6 and 7 of the ABO gene were sequenced. Results: 13 cases that had the cis-AB allele showed results suggestive of the cis-AB subgroup by manual methods, but were interpreted as AB by either automated device. This happened in 87.5% of these cases by QWALYS-3 and 70.0% by Galileo NEO. Genotyping results showed that 12 cases were ABO*cis-AB01/ABO*O01 or ABO*cis-AB01/ABO*O02, and one case was ABO*cis-AB01/ ABO*A102. Conclusion: Cis-AB samples were mistyped as AB by the automated microplate technique in some cases. We suggest that the manual tile method can be a simple supplemental test for the detection of the cis-AB phenotype, especially in countries with relatively high cis-AB prevalence.

show abstract

“…The cis-AB blood group is rare; however, it is relatively more common in Japanese and Korean populations. In apparent contradiction to the general Mendelian inheritance of ABO blood groups, the A and B characteristics are inherited together from one parent in the cis-AB blood group [12,13]. Yamaguchi [14] reported (based on the strength of serological reactions) that cis-AB has three phenotypes: A 2 B 3 , A 2 B and A 1 B 3 .…”

Section: Cis-ab Blood Group In Koreamentioning

confidence: 99%

“…So far, nine cis-AB alleles (cis-AB01, cis-AB01var, cis-AB02 to cis-AB08) have been reported in the Blood Group Antigen Gene Mutation Database [7]. Although these cis-AB alleles are found in various ethnic groups, they are mainly found in Asian populations [12][13][14]. The nucleotide sequence in exons 6 and 7 of cis-AB01 allele was identical to the A102 allele consensus allele except for the characteristic mutations (c.803G>C in exon 7) found in cis-AB01 allele.…”

Section: Cis-ab Blood Group In Koreamentioning

confidence: 99%

ABO subgroup studies in Korea

Cho

Lee

Ryang

2015

ISBT Science Series

Self Cite

View full text Add to dashboard Cite

Numerous examples of A or B subgroup alleles have been described in many geographical and ethnic groups. To analyse the phenotypic and genotypic basis of some ABO subgroups in Korea, a large-scale study was performed on 169 605 samples from Korean blood donors. The overall incidence of ABO subgroups was 0Á13% (227/169,605) in this donor population. Based on genotyping and/or serologic typing, the subgroups identified were as follows: A 2 B (n = 120), A 2 B 3 (n = 36), A 1 B 3 (n = 22), A 2 (n = 13), B 3 (n = 13), A int B 3 (n = 6) and others (n = 17). Amongst these individuals with ABO subgroups, 26Á4% (60/227) demonstrated a cis-AB01 allele. It has been reported that the Korean population has several unique ABO subgroups (Aw10, Aw14 and B306) showing allelic competition or allelic enhancement. Several congenital blood chimaeras have been also found when studying individuals with ABO subgroups. Like ABO subgroups showing the allelic enhancement and allelic competition, chimaerism is also an important cause of ABO phenotype-genotype discrepancies when implementing DNA-based blood grouping techniques. To understand the pattern of A and B antigen expression amongst donors with subtype alleles, flow cytometric analysis of ABO antigen expression on HeLa cells transfected with plasmids containing various mutants was used, in addition to ABO genotyping. ABO subgroup frequencies in KoreaThe human ABO blood group is determined by the ABO gene located on chromosome 9. This gene contains seven exons, of which the two largest exons (6 and 7) comprise 77% of the coding sequence of the gene and encode 91% of the catalytic domain. The gene encoding a glycosyltransferase (GT) can transfer either N-acetylgalactosamine (GalNAc) to create an A antigen (glycosyltransferase A, GTA) or galactose (Gal) to create a B antigen (glycosyltransferase B, GTB) to the acceptor H substance [1-4]. Of the common ABO alleles, A alleles (A101 and A201 are common alleles in Caucasians, but not in Asians; A102 is a common allele in Asians), B allele (B101) and O alleles (O01, O02 and O03; O03 is rare in Asians) have been reported [5][6][7][8][9][10][11]. In addition to the common ABO blood types, weak subgroup phenotypes (A 2 , A 3 , A x , A el , B 3 , B x , B(A) and cis-AB) have been identified [7]. It is well known that the phenotypic frequencies and genetic basis of ABO subgroups differ between ethnic regions. To analyse the ABO subgroup frequencies in Korea, a large-scale study was performed on 169 605 samples from Korean blood donors. The overall incidence of ABO subgroups was 0Á13% (227/169,605) in this donor population. Based on genotyping and/or serologic typing, the subgroups identified were as follows: A 2 B (n = 120), A 2 B 3 (n = 36), A 1 B 3 (n = 22), A 2 (n = 13), B 3 (n = 13), A int B 3 (n = 6), A w B (n = 5), A 1 B x or el (n = 5), A int (n = 2), A m or el (n = 2), A el (n = 2) and B m or x (n = 1) [12]. Cis-AB blood group in KoreaAmongst 0Á13% (227/169 605) ABO subgroups in the large-scale study of Korea, 26Á4% (60/227) had a cis-AB ...

show abstract

Unusual phenotype of cis‐AB

Cited by 11 publications

References 3 publications

The serological and genetic basis of the cis‐AB blood group in Korea

The serological and genetic basis of the cis‐AB blood group in Korea

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

ABO subgroup studies in Korea

Contact Info

Product

Resources

About