Unsaturated lipid isomeric imaging based on the Paternò–Büchi reaction in the solid phase in ambient conditions

Zhang, Jian; Guo, Changjie; Huo, Xinming; Ma, Xiaoxiao; Li, Xin; Abliz, Zeper; Chu, Yao; Wang, Xiaohao; Tang, Fei

doi:10.1016/j.talanta.2021.122816

Cited by 14 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the current state of the technology enables resolving sn and CC isomers in MSI experiments. An unexpectedly high variation of GPL isomer abundances in specific tissue regions and healthy versus tumor tissue was revealed by recent studies, pointing toward their relevance in biomedical and clinical applications. − …”

Section: Isomers Of Glycerophospholipidsmentioning

confidence: 99%

“…The work of Zhang et al showed an on-tissue PB reaction with benzophenone in the solid phase combined with air-flowassisted desorption electrospray ionization-mass spectrometry (AFADESI MS). 20 Benzophenone was sprayed on the sample and dried, forming a fine solid coating. PB reaction took place in the solid state under 254 nm UV light irradiation for 10 min.…”

Section: ■ Isomers Of Glycerophospholipidsmentioning

confidence: 99%

See 1 more Smart Citation

Mass Spectrometry Imaging Techniques Enabling Visualization of Lipid Isomers in Biological Tissues

et al. 2022

View full text Add to dashboard Cite

This Feature focuses on a review of recent developments in mass spectrometry imaging (MSI) of lipid isomers in biological tissues. The tandem MS techniques utilizing online and offline chemical derivatization procedures, ion activation techniques such as ozone-induced dissociation (OzID), ultraviolet photodissociation (UVPD), or electron-induced dissociation (EID), and other techniques such as coupling of ion mobility with MSI are discussed. The importance of resolving lipid isomers in diseases is highlighted.

show abstract

Section: Isomers Of Glycerophospholipidsmentioning

confidence: 99%

Section: ■ Isomers Of Glycerophospholipidsmentioning

confidence: 99%

Mass Spectrometry Imaging Techniques Enabling Visualization of Lipid Isomers in Biological Tissues

et al. 2022

View full text Add to dashboard Cite

show abstract

“…在反应室中, 苯甲醛蒸汽与氮气在样品组织上可形成一层均一的粘性薄膜, 在紫外光照射下发生 PB 反应, 随后即可进行 MALDI-MSI 分析. [118] 、二苯甲酮 [125] 与甜菜碱醛 [99] 在 DESI-MSI 原位分析中的应用有过相关报道. 脊髓损伤后, 病灶中心及邻近区域上脂质的相对强度由于脂质氧化或者过氧化而略有下降 [208,209] .…”

Section: F O R R E V I E W O N L Yunclassified

“…众所周知，不饱和脂质存在丰富的异构体, 不同脂质异构体的生理功能差异明显, 因此对不饱和脂质异构体进行有效定性定量研究极为重要. Zhang 等人 [125] [100] [99] 、猪肾上腺组织中胆固醇 [101] 以及动脉粥样硬化中 PLs 和胆固醇的空间分布 [100] 等 F o r R e v i e w O n l y…”

Section: F O R R E V I E W O N L Yunclassified

Chemical derivatization: recent advances in mass spectrometry imaging

Li¹,

Qin²,

Jiang³

et al. 2022

Sci. Sin.-Chim

View full text Add to dashboard Cite

Recently, mass spectrometry imaging (MSI) has been drawing more and more attention due to its continuous development and improvement as well as its vital role in the study of biological science. Many MSI-based strategies have been widely applied to the in situ qualitative and quantitative detection and imaging of endogenous/exogenous molecules in tissues. Among, matrix assisted laser desorption/ionization MSI (MALDI-MSI) and desorption electrospray ionization MSI (DESI-MSI) are generally regarded as two the most commonly used molecular imaging techniques. Although MSI, as a histology-based emerging molecular imaging technique, has significant advantages over other imaging techniques because it is a label-free technique which provides high sensitivity, high throughput, and molecular specificity derived from the use of mass spectrometers as detectors for a wide variety of ionized biomolecules in situ within a tissue section, it still has obvious shortcomings in the in situ analysis of low-polarity and neutral biomolecules. To solve this issue, the application of chemical derivatization technology in MSI is one of the most important approaches to overcome these deficiencies of MS molecular imaging. This review provides an overview of various chemical derivatization techniques, such as, esterification derivatization, acylation derivatization, addition reaction derivatization, substitution derivatization, oxidative derivatization, and other derivatizations, used in MS analysis, highlights the application of these chemical derivatization methods in the in situ detection and imaging of biomolecules in tissues by MALDI-MSI and DESI-MSI. It can be predicted that with the advance progress of chemical derivatization technology, the application scope of MSI will be further expanded in the fields of spatial metabolomics, spatial lipidomics, spatial proteomics, and spatial glycomics.

show abstract

“…In 2021, for lipid isomer identification, Unsihuay et al reported an online photochemical derivatization approach, i.e., 1 O 2 reacted with lipids to form lipid hydroperoxides (LOOHs), which was then followed by collision-induced dissociation (CID) to obtain diagnostic peaks (Figure 6H) [106]. The Paternò-Büchi (PB) reaction is another commonly used method for identifying the location of C=C double bonds in unsaturated lipids and simplified PB reaction conditions were introduced by spraying the reagent solution on the tissue surface with only 10 min preprocessing [107]. and H&E staining of normal tissue, IDC and DCIS samples.…”

Section: Lipidsmentioning

confidence: 99%

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Liu

et al. 2021

Metabolites

View full text Add to dashboard Cite

Ambient mass spectrometry imaging (AMSI) has attracted much attention in recent years. As a kind of unlabeled molecular imaging technique, AMSI can enable in situ visualization of a large number of compounds in biological tissue sections in ambient conditions. In this review, the developments of various AMSI techniques are discussed according to one-step and two-step ionization strategies. In addition, recent applications of AMSI for lipid and metabolite analysis (from 2016 to 2021) in disease diagnosis, animal model research, plant science, drug metabolism and toxicology research, etc., are summarized. Finally, further perspectives of AMSI in spatial resolution, sensitivity, quantitative ability, convenience and software development are proposed.

show abstract

Unsaturated lipid isomeric imaging based on the Paternò–Büchi reaction in the solid phase in ambient conditions

Cited by 14 publications

References 34 publications

Mass Spectrometry Imaging Techniques Enabling Visualization of Lipid Isomers in Biological Tissues

Mass Spectrometry Imaging Techniques Enabling Visualization of Lipid Isomers in Biological Tissues

Chemical derivatization: recent advances in mass spectrometry imaging

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Contact Info

Product

Resources

About