Unraveling the mechanism responsible for the contrasting tolerance of Synechocystis and Synechococcus to Cr(VI): Enzymatic and non-enzymatic antioxidants

Gupta, Alka; Ballal, Anand

doi:10.1016/j.aquatox.2015.04.015

Cited by 22 publications

(15 citation statements)

References 43 publications

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“…This suggests that further studies are necessary to determine whether these factors are present in our samples. In other species, ROS can target lipids and initiate the lipid peroxidation process, which may lead to molecular cell damage (Gupta & Ballal, 2015). In the present study, LAECs and Cs1 showed no differences compared to the negative control at all concentrations tested in the H 2 O 2 -induced lipid peroxidation assay, which indicates the absence of lipid oxidative damage and hence the potential antioxidant action of C. speciosa, consistent with the results of the DPPH test.…”

Section: Discussionsupporting

confidence: 77%

“…An imbalance between production and inactivation of ROS can lead to oxidative stress and damage to various biological macromolecules, and proteins exposed to ROS can suffer oxidative modifications that can lead to changes in their functions, and, consequently, affect cellular metabolism (Gupta, Ballal, 2015). In this context, measurement of protein carbonyl groups in biological samples is a reliable parameter with which to assess ROS-mediated protein oxidation (Levine et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

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Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Malheiros

Silva

Hofmann

et al. 2017

Braz. J. Pharm. Sci.

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The bark tea of Ceiba speciosa, a tropical tree of the Malvaceae family, is used in the Northwestern Region of Rio Grande do Sul state, Brazil, to reduce blood cholesterol levels. However, there are no scientific data on the efficacy and safety of this plant. The aim of the present study was to evaluate the in vitro antioxidant and toxic potential of bark extracts of C. speciosa. We performed a preliminary phytochemical analysis by high-performance liquid chromatography-diode array detection (HPLC-DAD) and evaluated the oxidative damage to proteins and lipids, the radical scavenging effect, and genotoxicity of the lyophilized aqueous extract (LAECs) and the precipitate obtained from the raw ethanol extract (Cs1). The phytochemical profile demonstrated the presence of phenolic and flavonoid compounds. The LAECs and Cs1 prevented damage to lipids and proteins at concentrations of 50 and 10 µg/mL. They also showed a scavenging effect on 2,2-diphenyl-1-pricril-hydrazyl (DPPH) radicals in a concentration-dependent manner. Furthermore, no genotoxic effect was observed at concentrations of 10, 5 and 2 µg/mL in the Comet assay. The present study is the first evaluation regarding the characterization of C. speciosa and its safety, and the results demonstrate its antioxidant potential and suggest that its therapeutic use may be relatively safe.Uniterms: Ceiba speciose/phytochemistry. Ceiba speciose/antioxidant activity. Phenolic content. Comet assay. DPPH scavenging capacity. Natural medicine. Safety.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Malheiros

Silva

Hofmann

et al. 2017

Braz. J. Pharm. Sci.

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“…The chlorophyll (Chl) a concentration of Synechocystis cells was determined as described previously ( Wintermans and De Mots, 1965 ; Knudson et al, 1977 ; Luo et al, 2013 ; Wang et al, 2013 ; Gupta and Ballal, 2015 ) with some modifications. Briefly, 1 ml cell culture was centrifuged at 6000 rpm for 4 min.…”

Section: Methodsmentioning

confidence: 99%

Probing Synechocystis-Arsenic Interactions through Extracellular Nanowires

et al. 2016

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Microbial nanowires (MNWs) can play an important role in the transformation and mobility of toxic metals/metalloids in environment. The potential role of MNWs in cell-arsenic (As) interactions has not been reported in microorganisms and thus we explored this interaction using Synechocystis PCC 6803 as a model system. The effect of half maximal inhibitory concentration (IC50) [~300 mM As (V) and ~4 mM As (III)] and non-inhibitory [4X lower than IC50, i.e., 75 mM As (V) and 1 mM As (III)] of As was studied on Synechocystis cells in relation to its effect on Chlorophyll (Chl) a, type IV pili (TFP)-As interaction and intracellular/extracellular presence of As. In silico analysis showed that subunit PilA1 of electrically conductive TFP, i.e., microbial nanowires of Synechocystis have putative binding sites for As. In agreement with in silico analysis, transmission electron microscopy analysis showed that As was deposited on Synechocystis nanowires at all tested concentrations. The potential of Synechocystis nanowires to immobilize As can be further enhanced and evaluated on a large scale and thus can be applied for bioremediation studies.

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“…In comparison, unstressed Synechocystis PCC 6803 (Banerjee et al, 2012b) and Synechococcus PCC 7942 (Gupta and Ballal, 2015) both show inherently higher levels of catalase, which can be easily detected on zymograms. This correlates well with the fact that both Synechocystis PCC 6803 and Synechococcus PCC 7942 tolerate more H 2 O 2 than Anabaena PCC 7120 (Pascual et al, 2010;Gupta and Ballal, 2015). Deletion of katG in Synechococcus and Figure 7.…”

Section: Discussionmentioning

confidence: 99%

A Salt-Inducible Mn-Catalase (KatB) Protects Cyanobacterium from Oxidative Stress

et al. 2015

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Unraveling the mechanism responsible for the contrasting tolerance of Synechocystis and Synechococcus to Cr(VI): Enzymatic and non-enzymatic antioxidants

Cited by 22 publications

References 43 publications

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Probing Synechocystis-Arsenic Interactions through Extracellular Nanowires

A Salt-Inducible Mn-Catalase (KatB) Protects Cyanobacterium from Oxidative Stress

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