Unraveling the complexity of transcriptomic, metabolomic and quality environmental response of tomato fruit

D’Esposito, Daniela; Ferriello, Francesca; Molin, Alessandra Dal; Diretto, Gianfranco; Sacco, Adriana; Minio, Andrea; Barone, Amalia; Monaco, Rossella Di; Cavella, Silvana; Tardella, Luca; Giuliano, Giovanni; Delledonne, Massimo; Frusciante, Luigi; Ercolano, Maria Raffaella

doi:10.1186/s12870-017-1008-4

Cited by 49 publications

(37 citation statements)

References 70 publications

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“…They also indicate the need for careful selection of crop genotypes that have robust agronomic, and other desirable traits, under different agroecosystems (Grobkinsky et al, 2015); that is, crop genotypes that result in consistent expression of traits across agroecosystems and environments. Metabolic networks across genotypes and agroecosystems highlight differences in the structure of primary metabolic networks and reveal the fluidity of plant metabolic networks; in agreement with the recent discussion on differential networks of plant metabolism (Omranian et al, 2015;D'Espito et al, 2017).…”

Section: Impact Of Agroecosystem and Crop Genotype On The Tomato Metasupporting

confidence: 84%

“…The hairy vetch cropping system has relatively lower soil temperatures, different N fertility, and an altered soil microbial community (Teasdale and Abdul-Baki, 1997;Buyer et al, 2010). Consistent with these findings, when three tomato cultivars were grown in a conventional production system at two field locations that varied in environmental conditions (average air temperature, soil texture, soil pH, soil mineral content) reprogramming of transcription and the metabolome occurs in a genotype-specific manner (D'Espito et al, 2017).…”

Section: Impact Of Agroecosystem and Crop Genotype On The Tomato Metamentioning

confidence: 55%

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Sustainable Crop Production Systems and Human Nutrition

Roberts

Mattoo

2019

Front. Sustain. Food Syst.

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There is a pressing need for redesigning agriculture to achieve sustainability and for utilizing modern genetic tools, including genetic engineering, to add nutritional value to crops for the benefit of the diverse human population. Collectively, plant foods contain most minerals, macronutrients (calories), and micronutrients essential for human nutrition. These plant foods also contain a range of bioactive compounds that can play important roles in the prevention of chronic diseases including heart disease, cancer, stroke, diabetes, Alzheimer's, cataracts, and age-related functional decline. However, these bioactive nutrients are often present at marginal concentrations in edible plant tissues in regard to human nutrition/prevention of disease. The quantity of these nutrients in edible plant tissues is primarily dependent on crop genetics and regulated by complex and overlapping mechanisms in response to developmental and environmental cues. Environmental cues are naturally occurring-temperature, light intensity, and other stressors, or result from crop production system components-fertilizer, tillage operations, etc. One strategy for sustainable, next-generation crop development is to design cropping systems that have minimal or lesser impact on the environment and to use genetic approaches to enhance crop nutritional content. This strategy is appealing since crop genetics is the primary driver of plant nutrient content and because purely managing crop production fields to optimize crop nutrient content is extremely challenging if not impossible. As an example we present the development of a next-generation sustainable tomato production system with beneficial impacts on tomato physiology and nutritional quality of the tomato fruit. Our analysis of the metabolomes of tomato cultivars in this and other cropping systems highlights the need for robust cultivars that consistently express nutritional and other desirable traits across cropping systems and under differing environmental conditions.

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Section: Impact Of Agroecosystem and Crop Genotype On The Tomato Metasupporting

confidence: 84%

Section: Impact Of Agroecosystem and Crop Genotype On The Tomato Metamentioning

confidence: 55%

Sustainable Crop Production Systems and Human Nutrition

Roberts

Mattoo

2019

Front. Sustain. Food Syst.

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“…Nonpolar (carotenoids) and semi-polar (phenylpropanoid) analyses were carried out by liquid chromatography coupled to diodearray detector and atmospheric pressure chemical ionizationhigh-resolution mass spectrometry (LC-DAD-APCI-HRMS) or electrospray ionization (LC-DAD-ESI-HRMS), respectively, operating in positive and negative ion modes, as previously described (D'Esposito et al, 2017;Fasano et al, 2016;Su et al, 2015). Identification of carotenoids was performed as reported previously (Liu et al, 2014).…”

Section: Nonpolar and Semi-polar Metabolite Analysesmentioning

confidence: 99%

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Diretto

Frusciante

Fabbri

et al. 2019

Plant Biotechnology Journal

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Summary Tomato fruit ripening is controlled by the hormone ethylene and by a group of transcription factors, acting upstream of ethylene. During ripening, the linear carotene lycopene accumulates at the expense of cyclic carotenoids. Fruit‐specific overexpression of LYCOPENE β‐CYCLASE (LCYb) resulted in increased β‐carotene (provitamin A) content. Unexpectedly, LCYb‐overexpressing fruits also exhibited a diverse array of ripening phenotypes, including delayed softening and extended shelf life. These phenotypes were accompanied, at the biochemical level, by an increase in abscisic acid (ABA) content, decreased ethylene production, increased density of cell wall material containing linear pectins with a low degree of methylation, and a thicker cuticle with a higher content of cutin monomers and triterpenoids. The levels of several primary metabolites and phenylpropanoid compounds were also altered in the transgenic fruits, which could be attributed to delayed fruit ripening and/or to ABA. Network correlation analysis and pharmacological experiments with the ABA biosynthesis inhibitor, abamine, indicated that altered ABA levels were a direct effect of the increased β‐carotene content and were in turn responsible for the extended shelf life phenotype. Thus, manipulation of β‐carotene levels results in an improvement not only of the nutritional value of tomato fruits, but also of their shelf life.

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“…The soluble fraction was analyzed by HPLC-DAD-HRMS and HPLC-DAD (Ahrazem et al, 2018; Moraga et al, 2009). The liposoluble fractions (crocetin, HTCC, -cyclocitral, carotenoids and chlorophylls) were extracted with 0.5:1 ml cold extraction solvents (50:50 methanol and CHCl 3 ), and analyzed by HPLC-DAD-HRMS and HPLC-DAD as previously described (Ahrazem et al, 2018; Castillo et al, 2005; D’Esposito et al, 2017; Fasano et al, 2016). Metabolites were identified on the basis of absorption spectra and retention times relative to standard compounds.…”

Section: Methodsmentioning

confidence: 99%

Efficient production of saffron crocins and picrocrocin inNicotiana benthamianausing a virus-driven system

Martí¹,

Diretto²,

Aragonés³

et al. 2019

Preprint

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22Crocins and picrocrocin are glycosylated apocarotenoids responsible, respectively, 23 for the color and the unique taste of the saffron spice, known as red gold due to its 24 high price. Several studies have also shown the health-promoting properties of these 25 compounds. However, their high costs hamper the wide use of these metabolites in 26 the pharmaceutical sector. We have developed a virus-driven system to produce 27 remarkable amounts of crocins and picrocrocin in adult Nicotiana benthamiana 28 plants in only two weeks. The system consists of viral clones derived from tobacco 29 etch potyvirus that express specific carotenoid cleavage dioxygenase (CCD) enzymes 30 from Crocus sativus and Buddleja davidii. Metabolic analyses of infected tissues 31 demonstrated that the sole virus-driven expression of C. sativus CsCCD2L or B. 32 davidii BdCCD4.1 resulted in the production of crocins, picrocrocin and safranal. 33 2 Using the recombinant virus that expressed CsCCD2L, accumulations of 0.2% of 34 crocins and 0.8% of picrocrocin in leaf dry weight were reached in only two weeks. 35 In an attempt to improve apocarotenoid content in N. benthamiana, co-expression 36 of CsCCD2L with other carotenogenic enzymes, such as Pantoea ananatis phytoene 37 synthase (PaCrtB) and saffron -carotene hydroxylase 2 (BCH2), was performed 38 using the same viral system. This combinatorial approach led to an additional crocin 39 increase up to 0.35% in leaves in which CsCCD2L and PaCrtB were co-expressed. 40 Considering that saffron apocarotenoids are costly harvested from flower stigma 41 once a year, and that Buddleja spp. flowers accumulate lower amounts, this system 42 may be an attractive alternative for the sustainable production of these appreciated 43 metabolites. 44 45

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Unraveling the complexity of transcriptomic, metabolomic and quality environmental response of tomato fruit

Cited by 49 publications

References 70 publications

Sustainable Crop Production Systems and Human Nutrition

Sustainable Crop Production Systems and Human Nutrition

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Efficient production of saffron crocins and picrocrocin inNicotiana benthamianausing a virus-driven system

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