Unpredictable recombination of PB transposon in Silkworm: a potential risk

Jia, Xuehua; Pang, Xiao‐Yu; Yuan, Ya-Jie; Gao, Qiang; Lü, Ming; Zhang, Guang‐Xian; Dai, Fangyin; Zhao, Tianfu

doi:10.1007/s00438-020-01743-0

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…The PB transposon allows a large cargo capacity for exogenous genes (Chapeau et al, 2017) and shows complete reversibility of genomic modifications without footprint (Woodard, Wilson, & Matthew, 2015). Traditional PB transposon systems are composed of transposon vectors (donor plasmids) and transposase expression vectors (helper plasmids), the possibility of active PBase gene insertions into the host genome exists and has rarely been addressed (Chang, Pan, Landrette, Ding, & Xu, 2019; Jia et al, 2021). PB transposase mRNA (PBase mRNA) instead of helper plasmids was regarded as an ideal method to avoid this risk (Sato, Inada, Saitoh, Watanabe, & Nakamura, 2020; Solenne et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Using a modified piggyBac transposon‐combined Cre/loxP system to produce selectable reporter‐free transgenic bovine mammary epithelial cells for somatic cell nuclear transfer

Song

Wang

et al. 2023

Genesis

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Summary Transposon systems are widely used for genetic engineering in various model organisms. PiggyBac (PB) has recently been confirmed to have highly efficient transposition in the mouse germ line and mammalian cell lines. In this study, we used a modified PB transposon system mediated by PB transposase (PBase) mRNA carrying the human lactoferrin gene driven by bovine β‐casein promoter to transfect bovine mammary epithelial cells (BMECs), and the selectable reporter in two stable transgenic BMEC clones was removed using cell‐permeant Cre recombinase. These reporter‐free transgenic BMECs were used as donor cells for somatic cell nuclear transfer (SCNT) and exhibited a competence of SCNT embryos similar to stable transgenic BMECs and nontransgenic BMECs. The comprehensive information from this study provided a modified approach using an altered PB transposon system mediated by PBase mRNA in vitro and combined with the Cre/loxP system to produce transgenic and selectable reporter‐free donor nuclei for SCNT. Consequently, the production of safe bovine mammary bioreactors can be promoted.

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