Universal sensor array for highly selective system identification using two-dimensional nanoparticles

Abstract

The universal sensor array is composed of 12 fluorescently silent non-specific artificial nanoreceptors (2D-nps) for the identification and classification of five proteins, three types of breast cancer cells and a structure-switching event of a macromolecule.

Nano‐Graphene Oxide Based Multichannel Sensor Arrays towards Sensing of Protein Mixtures

“…Depending on overall charge, functionality and extension of aromatic moieties in biomolecule as well as dyes can control the tunable interaction. By utilizing this non‐covalent interactions, several research group have made sensing platform by attaching fluorescent labeled single‐stranded DNA for detection of proteins, bacteria and diseased cell lines . But the biggest challenge to build a multichannel optical sensor array to select and optimize a set of fluorophores which will have minimum FRET and efficient binding as well as detectable displacement by analytes.…”

“…By utilizing this non-covalent interactions, severalr esearchg roup have made sensing platform by attaching fluorescent labeled single-strandedD NA for detection of proteins,b acteria and diseased cell lines. [27,29,30] But the biggest challenge to build am ultichannelo ptical sensora rray to select and optimize as et of fluorophores which will have minimum FRET and efficient bindinga sw ell as detectable displacement by analytes.H ence to build am ultichannel array by using nGO as ar eceptor first we have screened several fluorophores (Table S17) and finally we have selected five fluorophores 7-hydroxy coumarin (7-HC), Fluorescein (Fl), Rhodamine 6G (Rh-6G), Rhodamine Bb ase (Rh-B) and Methylene Blue (MB)] which not only have the diversec harge and functionality but also the excitation and emission wavelengths have nominal overlap to minimize the FRET (Figure 2b,c). The selected fluorophores which are used in this study,h ave good quantum yield (Table S1), water solubility and high photo stability.…”

Masking the Peroxidase‐Like Activity of the Molybdenum Disulfide Nanozyme Enables Label‐Free Lipase Detection

“…Prior to the detection studies, the MoS 2 2D‐nps were prepared from raw multilayered MoS 2 . The characteristic absorbance bands of the MoS 2 2D‐nps were measured and recorded by using absorbance spectroscopy (Figure S1 A in the Supporting Information) . The hydrodynamic radius, approximately 59 nm (% Pd: 21), was measured by using dynamic light scattering (Figure S1 B).…”

“…The resulting two‐dimensional MoS 2 nanoparticles exhibit graphene‐like properties at biological interfaces, particularly at DNA and 2D‐np surface interfaces, and this has been useful for the development of fluorometric sensors . We and others have used MoS 2 and other 2D‐nps for the development of nanosensor constructs and combinatorial sensor arrays for the fluorometric detection of DNA, RNA, proteins, cancer cells, and other compounds . What is more, label‐free colorimetric detection by using 2D‐nps is rather interesting from a simplicity standpoint.…”

“…We use this approach for the identification and discrimination of lipase both qualitatively, with visual inspection, and quantitatively, by using absorbance readings and a statistical analysis tool. Partial least‐squares discriminant analysis (PLSDA), which is a statistical analysis method for the discrimination of a group of inputs with distinct responses, is employed to discriminate lipase from the rest of the proteins tested and in an unknown analysis …”