Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags

Tu, Zhijie; Cheng, Siyun; Dong, Hao; Wang, Wenqi; Yang, Xingsheng; Gu, Bing; Wang, Shengqi; Wang, Chongwen

doi:10.1039/d2ra04735g

Cited by 14 publications

(15 citation statements)

References 51 publications

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“…An alternative approach is the use of SERS tags to achieve a sensitive detection with the spectra of Raman reporter molecules as label‐based methods. The SERS tags are usually composed of nanoparticles modified with capture probes to target bacteria with a Raman reporter molecule 33–35 . This approach has the potential to detect multiple bacteria simultaneously.…”

Section: Bacterial Detectionmentioning

confidence: 99%

“…Raman spectroscopy can be used with other technologies to improve the performance of the assay, such as lateral flow assays (LFAs) and microfluidics. Several studies combined SERS with lateral flow immunoassay for the detection of different bacteria 34,41,43 . Lateral flow assays offer the advantages of low costs, real‐time analysis, and portability.…”

Section: Bacterial Detectionmentioning

confidence: 99%

“…The SERS tags are usually composed of nanoparticles modified with capture probes to target bacteria with a Raman reporter molecule. [33][34][35] This approach has the potential to detect multiple bacteria simultaneously. The examples of these two types of strategies are shown in Novel SERS substrates were developed in some studies with a large specific surface area, rich porous structure, or multi-dimensional structure to improve the sensitivity and bacteria capture effi-ciency, such as nanoparticles, [35][36][37][38][39] nanosheets, [40][41][42] nanostickers, 43 nanoballoons, 44 and nanopancakes.…”

Section: Bacterial Detectionmentioning

confidence: 99%

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Recent advances of Raman spectroscopy for the analysis of bacteria

Rodriguez

Zhang

Wang

2023

Analytical Science Advances

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Rapid and sensitive bacteria detection and identification are becoming increasingly important for a wide range of areas including the control of food safety, the prevention of infectious diseases, and environmental monitoring. Raman spectroscopy is an emerging technology which provides comprehensive information for the analysis of bacteria in a short time and with high sensitivity. Raman spectroscopy offers many advantages including relatively simple operation, non‐destructive analysis, and information on molecular differences between bacteria species and strains. A variety of biochemical properties can be measured in a single spectrum. This short review covers the recent advancements and applications of Raman spectroscopy for bacteria analysis with specific focuses on bacteria detection, bacteria identification and discrimination, as well as bacteria antibiotic susceptibility testing in 2022. The development of novel substrates, the combination with other techniques, and the utilization of advanced data processing tools for the improvement of Raman spectroscopy and future directions are discussed.

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Section: Bacterial Detectionmentioning

confidence: 99%

Section: Bacterial Detectionmentioning

confidence: 99%

Section: Bacterial Detectionmentioning

confidence: 99%

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Recent advances of Raman spectroscopy for the analysis of bacteria

Rodriguez

Zhang

Wang

2023

Analytical Science Advances

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“…This interface creates new possibilities for producing a POC device for rapid clinical caries screening in future and demonstrates wide ranging potential in the clinical diagnosis of dental diseases. Tu et al [ 139 ] reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) using wheat germ agglutinin-modified, Au-modified magnetite nanocomposites for the detection of foodborne bacteria in food and environmental samples with high sensitivity. The proposed universal detection method was effectively utilized for the accurate and specific detection of three foodborne bacteria with a low detection limit (10 cells mL −1 ) and a short testing time (<8.14%).…”

Section: Diagnostic Application Of Mnpsmentioning

confidence: 99%

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

et al. 2023

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In the last few decades, point-of-care (POC) sensors have become increasingly important in the detection of various targets for the early diagnostics and treatment of diseases. Diverse nanomaterials are used as building blocks for the development of smart biosensors and magnetite nanoparticles (MNPs) are among them. The intrinsic properties of MNPs, such as their large surface area, chemical stability, ease of functionalization, high saturation magnetization, and more, mean they have great potential for use in biosensors. Moreover, the unique characteristics of MNPs, such as their response to external magnetic fields, allow them to be easily manipulated (concentrated and redispersed) in fluidic media. As they are functionalized with biomolecules, MNPs bear high sensitivity and selectivity towards the detection of target biomolecules, which means they are advantageous in biosensor development and lead to a more sensitive, rapid, and accurate identification and quantification of target analytes. Due to the abovementioned properties of functionalized MNPs and their unique magnetic characteristics, they could be employed in the creation of new POC devices, molecular logic gates, and new biomolecular-based biocomputing interfaces, which would build on new ideas and principles. The current review outlines the synthesis, surface coverage, and functionalization of MNPs, as well as recent advancements in magnetite-based biosensors for POC diagnostics and some perspectives in molecular logic, and it also contains some of our own results regarding the topic, which include synthetic MNPs, their application for sample preparation, and the design of fluorescent-based molecular logic gates.

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“…3,4 Notably, it poses a significant risk to global food safety, public health and can result in substantial economic losses. 5 The listeriosis cases have surged yearly, with up to 30% mortality rate for Listeria sepsis among the high-risk groups, including children, the elderly, pregnant women, and patients with malignancy, organ transplants, or other immunocompromised states. 6 Listeria sepsis represents onethird of adult cases of invasive listeriosis.…”

Section: ■ Introductionmentioning

confidence: 99%

The Evaluation of a Lateral Flow Strip Based on the Covalently Fixed “End-On” Orientation of an Antibody for Listeria monocytogenes Detection

Thongmee,

Ngernpimai,

Srichaiyapol

et al. 2024

Anal. Chem.

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In this study, the covalently fixed “end-on” orientation of a monoclonal Listeria monocytogenes antibody (mAb-Lis) to amino terminated oligo (ethylene glycol)-capped gold nanoparticles (NH2-TEG-AuNPs) was used to fabricate an in-house lateral flow strip (LFS), namely, the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS. The aim was to evaluate the performance of the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS in detecting L. monocytogenes. The proposed LFS enabled the sensitive detection of L. monocytogenes in 15 min with a visual limit of detection of 102 CFU/mL. Quantitative analysis indicated an LOD at 10 CFU/mL. The fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS showed no cross-reactivity with other pathogenic bacteria and practical performance across different food matrices, including human blood, milk, and mushroom samples. Furthermore, the clinical performance of the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS for detecting L. monocytogenes was evaluated by using 12 clinical samples validated by the hemoculture method. It demonstrated excellent concordance with the reference methods, with no false-positive or false-negative results observed. Therefore, the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS serves as a promising candidate for a point-of-care test (POCT), enabling the rapid, precise, and highly sensitive detection of L. monocytogenes in clinical samples and contaminated food.

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Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags

Abstract: A universal SERS-based lateral flow assay was proposed for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin-modified Fe3O4@Au nanotags.

Cited by 14 publications

References 51 publications

Recent advances of Raman spectroscopy for the analysis of bacteria

Recent advances of Raman spectroscopy for the analysis of bacteria

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

The Evaluation of a Lateral Flow Strip Based on the Covalently Fixed “End-On” Orientation of an Antibody for Listeria monocytogenes Detection

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